FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\brpnude
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General Information
Symbol
Dmel\brpnude
Species
D. melanogaster
Name
FlyBase ID
FBal0267420
Feature type
allele
Associated gene
Dmel\brp
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Amino acid replacement: ?1724term.

    (Hallermann et al., 2010)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2R:9,537,056..9,537,056 [+]
    Nucleotide change:

    C9537056T

    Amino acid change:

    Q1723term | brp-PD; Q1723term | brp-PE; Q1769term | brp-PG; Q1723term | brp-PH; Q1380term | brp-PI; Q2221term | brp-PJ; Q1690term | brp-PK; Q1764term | brp-PL; Q1481term | brp-PM; Q2226term | brp-PN; Q1660term | brp-PO; Q1776term | brp-PP; Q1778term | brp-PQ; Q1765term | brp-PR

    Reported amino acid change:

    ?1724term

    Comment:

    Nonsense mutation at at amino acid position 1724 (out of 1740). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    (Hallermann et al., 2010)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        brpnude/brp69 larvae have significantly reduced locomotion, compared to controls; they also have significantly reduced numbers of synaptic vesicles around motor neuron T-bars and significantly reduced NMJ presynaptic active zone area, compared to controls, with no effect on the number of presynaptic active zones; there is no effect on the rate of miniature excitatory postsynaptic currents (minis) in muscle 6 at the NMJ or on excitatory postsynaptic current (eEPSC) amplitude or decay time constant, compared to controls, but paired-pulse depression of eEPSCs is significantly increased, compared to controls.

        brpnude/+ larvae have significantly increased locomotion, compared to controls.

        (Scholz et al., 2019)

        The area of the active zone cytomatrix at mutant active zones is reduced compared to controls (assayed using direct stochastic optical reconstruction microscopy).

        Both paired pulse-stimulation and high-frequency trains of activity produced pronounced short-term depression of evoked excitatory postsynaptic currents in mutant larval neuromuscular junctions. The fast phase of recovery after high-frequency stimulation is slowed in the mutant compared to controls, while the slow recovery phase is normal.

        (Ehmann et al., 2014)

        brpnude mutants exhibit significantly reduced survival rates and motor abilities.

        brpnude mutant neuromuscular junctions exhibit a normal number of active zones and normal cytomatrix distribution, with wild-type levels of dense bodies. Furthermore, the diameter of the synaptic vesicles as well as the total number of synaptic vesicles per bouton section are unchanged in brpnude mutants.

        brpnude mutant synapses display normal Ca[2+] channel distribution.

        EPSCs evoked at 0.2Hz at brpnude mutant synapses do not differ in terms of their peak amplitude, rise time, or decay time constant from those of controls.

        Depression decay during paired-pulse stimulation is stronger in brpnude mutants, as quantified by a reduction in the steady-state EPSC amplitude at the end of the train to 35% compared to 52% in controls. Consistent with the altered paired-pulse ratio, the amplitude of the second EPSC in the train is already significantly reduced to 80% at brpnude synapses.

        brpnude mutant synapses display enhanced depression during sustained high frequency transmission. Consistently, the first component of recovery is slower, whilst the second component is unaltered.

        (Hallermann et al., 2010)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Enhanced by
        NOT Enhanced by
        Statement
        Reference

        brp69/brpnude has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by cpxSH1/cpx1257

        (Scholz et al., 2019)
        NOT suppressed by
        Statement
        Reference

        brp69/brpnude has abnormal neuroanatomy | larval stage phenotype, non-suppressible by cpxSH1/cpx1257

        (Scholz et al., 2019)
        NOT Enhancer of
        Statement
        Reference

        brp69/brpnude is a non-enhancer of abnormal locomotor behavior | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)
        Suppressor of
        Statement
        Reference

        brp69/brpnude is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)
        NOT Suppressor of
        Statement
        Reference

        brp69/brpnude is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)
        Other
        Phenotype Manifest In
        Enhanced by
        NOT Enhanced by
        Statement
        Reference

        brp69/brpnude has synaptic vesicle | larval stage phenotype, non-enhanceable by cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude has T-bar | larval stage phenotype, non-enhanceable by cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude has presynaptic active zone | larval stage phenotype, non-enhanceable by cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude has embryonic/larval motor neuron phenotype, non-enhanceable by cpxSH1/cpx1257

        (Scholz et al., 2019)
        NOT suppressed by
        Statement
        Reference

        brp69/brpnude has synaptic vesicle | larval stage phenotype, non-suppressible by cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude has T-bar | larval stage phenotype, non-suppressible by cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude has presynaptic active zone | larval stage phenotype, non-suppressible by cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude has embryonic/larval motor neuron phenotype, non-suppressible by cpxSH1/cpx1257

        (Scholz et al., 2019)
        NOT Enhancer of
        Statement
        Reference

        brp69/brpnude is a non-enhancer of synaptic vesicle | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude is a non-enhancer of T-bar | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)
        Suppressor of
        NOT Suppressor of
        Statement
        Reference

        brp69/brpnude is a non-suppressor of synaptic vesicle | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)

        brp69/brpnude is a non-suppressor of T-bar | larval stage phenotype of cpxSH1/cpx1257

        (Scholz et al., 2019)
        Other
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        brpnude/+; cpxSH1/+ double heterozygous larvae have significantly reduced locomotion, compared to controls, unlike either heterozygote alone.

        brpnude/brp69; cpx1257/cpxSH1 double mutants have suppression of the increased eEPSC amplitude and mini frequency of cpx1257/cpxSH1 mutant larval muscle 6 and enhancement of the paired-pulse depression seen in both brpnude/brp69 mutants and cpx1257/cpxSH1 mutants.

        (Scholz et al., 2019)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        brp5.38
        (Peled et al., 2014)
        brp5.38
        (Hallermann et al., 2010)
        brpnude
        (Petzoldt et al., 2020, Scholz et al., 2019, Ehmann et al., 2014, Hallermann et al., 2010)
        Name Synonyms
        Secondary FlyBase IDs
          References (5)