FlyBase Allele Report: Dmel\foxo[GS9928]

General Information

Symbol

Dmel\foxo^GS9928

Species

D. melanogaster

Name

FlyBase ID

FBal0193819

Feature type

allele

Associated gene

Dmel\foxo

Associated Insertion(s)

P{GSV6}foxo^GS9928

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Goberdhan et al., 2005)

Progenitor genotype

Associated Insertion(s)

P{GSV6}foxo^GS9928

Cytology

Description

P{GSV6} insertion in the first intron of foxo.

(Goberdhan et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{GSV6}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:14,057,314..14,057,314

(Vereshchagina et al., 2008, Goberdhan et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

ommatidium, with Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Detailed Description

Statement

Reference

Flies expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF show a smaller eye with disrupted eye shape.

(Vereshchagina et al., 2008)

Overexpression of foxo^GS9928, under the control of Scer\GAL4^GMR.PF, produces distinctive ommatidial loss, particularly in the posteroventral part of the eye.

(Goberdhan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has visible phenotype, enhanceable by wdb^GS9548/Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928 has visible phenotype, enhanceable by wdb^EP3559/Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has visible phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928, wdb^GS9548 has visible phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928, wdb^GS9548 has visible phenotype, suppressible by mts[+]/mts⁰²⁴⁹⁶

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928 has visible phenotype, suppressible by mts^DN.UAS.cUa/Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has visible phenotype, non-suppressible by mts[+]/mts⁰²⁴⁹⁶

(Vereshchagina et al., 2008)

Other

Statement

Reference

Rheb^AV4, Scer\GAL4^GMR.PF, foxo^GS9928 has lethal | male | pupal stage phenotype

(Goberdhan et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has eye phenotype, enhanceable by wdb^GS9548/Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928 has eye phenotype, enhanceable by wdb^EP3559/Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, enhanceable by Scer\GAL4^GMR.PF, acs^GS10666

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, enhanceable by path^GS13857/Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, enhanceable by Rheb^AV4

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, enhanceable by S6k^UAS.cWa, Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, enhanceable by path^UAS.cGa/mTor^{WT.UAS.Tag:FLAG}/Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, non-enhanceable by mTor^{WT.UAS.Tag:FLAG}/Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has eye phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928, wdb^GS9548 has eye phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928, wdb^GS9548 has eye phenotype, suppressible by mts[+]/mts⁰²⁴⁹⁶

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928 has eye phenotype, suppressible by mts^DN.UAS.cUa/Scer\GAL4^GMR.PF

(Vereshchagina et al., 2008)

Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF, foxo^GS9928 has ommatidium phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, acs^GS10666, foxo^GS9928 has ommatidium phenotype, suppressible by Scer\GAL4^GMR.PF/Akt^UAS.cSa

(Goberdhan et al., 2005)

Scer\GAL4^GMR.PF, foxo^GS9928, path^GS13857 has ommatidium phenotype, suppressible by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Goberdhan et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^GS9928 has eye phenotype, non-suppressible by mts[+]/mts⁰²⁴⁹⁶

(Vereshchagina et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Akt1^Scer\UAS.cSa in animals expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF completely suppresses the eye phenotype.

Expression of wdb^GS9548 in animals expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF dramatically enhances the eye phenotype. This effect is completely suppressed by co-expression of Akt1^Scer\UAS.cSa.

Expression of wdb^EP3559 in animals expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF enhances the eye phenotype.

mts⁰²⁴⁹⁶/+ has little effect on the eye phenotype of animals expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF.

mts⁰²⁴⁹⁶/+ suppresses the eye phenotype of animals expressing wdb^GS9548 and foxo^GS9928 under the control of Scer\GAL4^GMR.PF.

Expression of mts^DN.Scer\UAS in animals expressing foxo^GS9928 under the control of Scer\GAL4^GMR.PF completely suppresses the eye phenotype.

(Vereshchagina et al., 2008)

Co-expression of Akt1^Scer\UAS.cSa suppresses the Scer\GAL4^GMR.PF>foxo^GS9928 phenotype.

Co-expression of the following transgenes with Scer\GAL4^GMR.PF>foxo^GS9928 results in an enhancement of the ommatidial phenotype: Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, CG1139^GS10666 and path^GS13857. Akt1^Scer\UAS.cSa is able to express the combined effects of these transgenes so that eyes appear normal.

Co-expression of Rheb^AV4 with Scer\GAL4^GMR.PF>foxo^GS9928 leads to the loss of virtually all ommatidia in females. In contrast, males of this genotype suffer massive tissue degeneration in the head as late pupae and die at this stage.

Co-expression of S6k^Scer\UAS.cWa strongly enhances the Scer\GAL4^GMR.PF>foxo^GS9928 ommatidial phenotype.

Expression of Tor^{WT.Scer\UAS.T:Zzzz\FLAG} does not affect the Scer\GAL4^GMR.PF>foxo^GS9928 phenotype.

Co-expression of path^Scer\UAS.cGa and Tor^{WT.Scer\UAS.T:Zzzz\FLAG} together enhance the Scer\GAL4^GMR.PF>foxo^GS9928 reduced eye phenotype.

(Goberdhan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type