Third instar larval eye-antennal imaginal disc clones co-expressing dlg1GD4689 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.CoinFLP-FRT, in combination with Dicer-2 for efficient RNAi, form invasive tumors and induce autophagosomes (assessed by a Atg8a fluorescence reporter) in a cell non-autonomous manner in neighboring disc tissue, which are suppressed and not suppressed, respectively, by the additional clonal co-expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.
Overexpression of CdsAEY08412 partially, but significantly, rescues the small cell size phenotype found in flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC (when both are expressed in the salivary gland and fat body, under the control of Scer\GAL4ppl.PP). Moreover, the salivary gland ectopic lipid storage phenotype found in Pi3K92ED954A.Scer\UAS.T:Hsap\MYC-expressing flies can be fully suppressed by CdsAEY08412 overexpression.
Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation. No difference is seen in white prepupae.
In larvae co-expressing CaMKIIT287D.Scer\UAS and Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4D42, the CaMKIIT287D.Scer\UAS-dependent increase in bouton number is completely suppressed and is decreased to a value indistinguishable from that conferred by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC alone.
Heterozygosity for ebi2g2, ebiE90, ebiCC3, ebi2g1, ebiCCS-8, ebiE4, ebiΔ1, ebiW418X, ebik16213, ebiWKS-24 or ebiP7 enhances the small wing phenotype induced by Scer\GAL4Bx-MS1096-mediated expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.
foxoΔ94 mutant flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.Switch.PT all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).
foxoΔ94 mutant flies expressing Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 all survive for longer on food supplemented with 20mM paraquat compared to controls (but shorter compared to expression in a wild-type background).
The downward curvature of the wing blade caused by expression of banUY3207, banUY1678, banUY789 or banUY1079 under the control of Scer\GAL4Bx-MS1096 is suppressed by co-expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.