FlyBase Allele Report: Dmel\ebi[E4]

General Information

Symbol

Dmel\ebi^E4

Species

D. melanogaster

Name

FlyBase ID

FBal0096973

Feature type

allele

Associated gene

Dmel\ebi

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Dong et al., 1999)

Mutagen

ethyl methanesulfonate

(Dong et al., 1999)

Progenitor genotype

Cytology

Description

Amino acid replacement: M1I.

(Dong et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:415,391..415,391 [+]

Nucleotide change:

G415391C

Amino acid change:

M1I | ebi-PA

(Dong et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size (with ebi^P7)

(Marygold et al., 2011)

cell lethal | larval stage | somatic clone

(Marygold et al., 2011)

lethal | recessive

(Marygold et al., 2011)

lethal - all die during embryonic stage | recessive

(Dong et al., 1999)

partially lethal - majority die (with ebi^P7)

(Marygold et al., 2011)

some die during embryonic stage | recessive

(Dong et al., 1999)

Phenotype Manifest In

cone cell (with ebi^P7)

(Tsuda et al., 2002)

cone cell | somatic clone

(Tsuda et al., 2002)

embryo

(Dong et al., 1999)

embryonic/larval crystal cell

(Milchanowski et al., 2004)

embryonic epidermis

(Dong et al., 1999)

eye photoreceptor cell | somatic clone

(Dong et al., 1999)

filzkorper

(Dong et al., 1999)

ommatidium | somatic clone

(Dong et al., 1999)

ventral denticle belt

(Dong et al., 1999)

wing (with ebi^P7)

(Marygold et al., 2011)

wing pouch (with ebi^P7)

(Marygold et al., 2011)

Detailed Description

Statement

Reference

ebi^P7/ebi^E4 wings are 86% the size of control wings. Gross defects in wing patterning are not apparent. There is no significant decrease in the size of other organs or the whole organism.

ebi^P7/ebi^E4 larval wing discs have visibly smaller wing pouches.

No or very few ebi^E4 clones survive to late third instar stage when induced at 48 hours after egg deposition (AED), and survival is not improved by inducing clones in a Minute/+ background. Clones induced at 72 hours AED do survive, but are very small.

(Marygold et al., 2011)

The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.

(Milchanowski et al., 2004)

In ebi^E4/ebi⁷ animals at the non-permissive temperature a loss of cone cells is seen. When homozygous ebi^E4 clones are made in the developing eye, these mutant cells can differentiate into cone cells when they have wild-type neighbours.

(Tsuda et al., 2002)

Mutant embryos exhibit a tail up or U-shaped phenotype, with head defects. When there is no maternal contribution of ebi⁺ gene product, the embryos have a more severe phenotype, including a tightly curled morphology and the loss of ventral denticle belts. Severe head defects also occur. In contrast to Egfr mutants, some residual cuticular structures remain. Filzkorper are present but malformed. In mutant clones in the eye, each ommatidium contains a single R8 cell, and early clusters appear normal. Clusters containing 8 neurons do form, but disorganised clusters with fewer differentiated neurons also occur. Generally R cell number is reduced, though R7 cells do develop, and 80% of R cells show altered cellular morphology.

(Dong et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ebi[+]/ebi^E4 is an enhancer of abnormal size phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Marygold et al., 2011)

ebi[+]/ebi^E4 is an enhancer of abnormal size phenotype of Scer\GAL4^Bx-MS1096, aos^UAS.cMa

(Marygold et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

ebi^E4/ebi^P7 has cone cell phenotype, suppressible by Delta^UAS.cDa/Scer\GAL4^elav.PLu

(Tsuda et al., 2002)

Enhancer of

Statement

Reference

ebi[+]/ebi^E4 is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, egr^GS11687

(Lim et al., 2012)

ebi[+]/ebi^E4 is an enhancer of wing vein phenotype of Scer\GAL4^Bx-MS1096, aos^UAS.cMa

(Marygold et al., 2011)

ebi[+]/ebi^E4 is an enhancer of wing margin phenotype of sd^ETX4

(Marygold et al., 2011)

ebi[+]/ebi^E4 is an enhancer of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Marygold et al., 2011)

ebi^E4 is an enhancer of wing phenotype of N^nd-1

(Tsuda et al., 2002)

ebi^E4 is an enhancer of wing phenotype of N^55e11

(Tsuda et al., 2002)

ebi^E4 is an enhancer of wing phenotype of Ser¹

(Tsuda et al., 2002)

ebi^E4 is an enhancer of wing vein | ectopic phenotype of Delta^9P

(Tsuda et al., 2002)

ebi[+]/ebi^E4 is an enhancer of ommatidium phenotype of Sos^JC2, sev⁶

(Dong et al., 1999)

ebi[+]/ebi^E4 is an enhancer of photoreceptor cell R7 phenotype of Sos^JC2, sev⁶

(Dong et al., 1999)

ebi[+]/ebi^E4 is an enhancer of ommatidium phenotype of Sos^JC2/Sos[+], sev⁶

(Dong et al., 1999)

ebi[+]/ebi^E4 is an enhancer of photoreceptor cell R7 phenotype of Sos^JC2/Sos[+], sev⁶

(Dong et al., 1999)

ebi^E4 is an enhancer of eye phenotype of rux³

(Dong et al., 1999)

Suppressor of

Statement

Reference

ebi^E4 is a suppressor of sex comb phenotype of Pc¹

(Strübbe et al., 2011)

ebi^E4 is a suppressor of metathoracic leg phenotype of Pc¹

(Strübbe et al., 2011)

ebi^E4 is a suppressor of mesothoracic leg phenotype of Pc¹

(Strübbe et al., 2011)

Other

Statement

Reference

ebi^E4, vg¹ has wing vein phenotype

(Marygold et al., 2011)

ebi^E4, vg¹ has wing margin phenotype

(Marygold et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for ebi^E4 enhances the small wing phenotype induced by Scer\GAL4^Bx-MS1096-mediated expression of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}.

Heterozygosity for ebi^E4 enhances the small wing and wing vein loss phenotypes induced by Scer\GAL4^Bx-MS1096-mediated expression of aos^Scer\UAS.cMa.

Flies heterozygous for both vg¹ and ebi^E4 display wing notching and wing venation phenotypes that are not observed in any single heterozygote.

Heterozygosity for ebi^E4 enhances the notched wing margin phenotype seen in sd^ETX4 hemizygous males.

(Marygold et al., 2011)

In sev⁶ hemizygotes that are also heterozygous for Sos^JC2, 14.7% of ommatidia contain R7 cells. This phenotype is dominantly enhanced by ebi^E4.

(Dong et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ebi^E4 is rescued by ebi^+t7.5

(Marygold et al., 2011)

ebi^E4/ebi^P7 is rescued by ebi^UAS.cTa/Scer\GAL4^elav.PLu

(Tsuda et al., 2002)

Comments

ebi^+t7.5 rescues the homozygous lethality of ebi^E4.

(Marygold et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Null allele.

(Dong et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ebi E4

(Strübbe et al., 2011)

ebi⁴

(Lim et al., 2012)

ebi^E4

(Nguyen et al., 2016, Lim et al., 2012, Marygold et al., 2011, Tsuda et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

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