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General Information
Symbol
Dmel\ebiE4
Species
D. melanogaster
Name
FlyBase ID
FBal0096973
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G415391C

Amino acid change:

M1I | ebi-PA

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: M1I. Nucleotide substitution: the ATG start codon is changed to ATC.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ebiP7/ebiE4 wings are 86% the size of control wings. Gross defects in wing patterning are not apparent. There is no significant decrease in the size of other organs or the whole organism.

ebiP7/ebiE4 larval wing discs have visibly smaller wing pouches.

No or very few ebiE4 clones survive to late third instar stage when induced at 48 hours after egg deposition (AED), and survival is not improved by inducing clones in a Minute/+ background. Clones induced at 72 hours AED do survive, but are very small.

The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.

In ebiE4/ebi7 animals at the non-permissive temperature a loss of cone cells is seen. When homozygous ebiE4 clones are made in the developing eye, these mutant cells can differentiate into cone cells when they have wild-type neighbours.

Mutant embryos exhibit a tail up or U-shaped phenotype, with head defects. When there is no maternal contribution of ebi+ gene product, the embryos have a more severe phenotype, including a tightly curled morphology and the loss of ventral denticle belts. Severe head defects also occur. In contrast to Egfr mutants, some residual cuticular structures remain. Filzkorper are present but malformed. In mutant clones in the eye, each ommatidium contains a single R8 cell, and early clusters appear normal. Clusters containing 8 neurons do form, but disorganised clusters with fewer differentiated neurons also occur. Generally R cell number is reduced, though R7 cells do develop, and 80% of R cells show altered cellular morphology.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

ebi[+]/ebiE4 is an enhancer of eye phenotype of Scer\GAL4GMR.PU, egrGS11687

ebi[+]/ebiE4 is an enhancer of wing vein phenotype of Scer\GAL4Bx-MS1096, aosUAS.cMa

ebi[+]/ebiE4 is an enhancer of wing margin phenotype of sdETX4

ebiE4 is an enhancer of wing phenotype of Nnd-1

ebiE4 is an enhancer of wing phenotype of N55e11

ebiE4 is an enhancer of wing phenotype of SerD

ebiE4 is an enhancer of wing vein | ectopic phenotype of Dl9P

ebi[+]/ebiE4 is an enhancer of ommatidium phenotype of SosJC2, sev6

ebi[+]/ebiE4 is an enhancer of photoreceptor cell R7 phenotype of SosJC2, sev6

ebi[+]/ebiE4 is an enhancer of ommatidium phenotype of SosJC2/Sos[+], sev6

ebi[+]/ebiE4 is an enhancer of photoreceptor cell R7 phenotype of SosJC2/Sos[+], sev6

ebiE4 is an enhancer of eye phenotype of rux3

Suppressor of
Statement
Reference

ebiE4 is a suppressor of sex comb phenotype of Pc1

ebiE4 is a suppressor of metathoracic leg phenotype of Pc1

ebiE4 is a suppressor of mesothoracic leg phenotype of Pc1

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Heterozygosity for ebiE4 enhances the small wing phenotype induced by Scer\GAL4Bx-MS1096-mediated expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC.

Heterozygosity for ebiE4 enhances the small wing and wing vein loss phenotypes induced by Scer\GAL4Bx-MS1096-mediated expression of aosScer\UAS.cMa.

Flies heterozygous for both vg1 and ebiE4 display wing notching and wing venation phenotypes that are not observed in any single heterozygote.

Heterozygosity for ebiE4 enhances the notched wing margin phenotype seen in sdETX4 hemizygous males.

In sev6 hemizygotes that are also heterozygous for SosJC2, 14.7% of ommatidia contain R7 cells. This phenotype is dominantly enhanced by ebiE4.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

ebi+t7.5 rescues the homozygous lethality of ebiE4.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Null allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)