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General Information
Symbol
Dmel\Nnd-1
Species
D. melanogaster
Name
FlyBase ID
FBal0012890
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
nd1, nd, NND1
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Reported as a 41 nucleotide deletion at the 3' end of the 8th exon. The sequence of the 41 deleted nucleotides was reported. The strain also contains the T to C mutation in the N 3' UTR present in the progenitor Nnd-1-dse chromosome.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Contains both the the 41 bp deletion within the N coding region described by Harding-Theobald et al. 2007 (FBrf0195930) and the mutation present in the progenitor Nnd-1-dse chromosome (T9408C substitution within the Downstream Sequence Element (DSE) of the consensus polyadenylation site).

41bp deletion at the 3' end of the 8th exon, within the intracellular domain. The deleted nucleotides, as determined by sequencing genomic DNA, are CGGTTCAGCGGGCTTGGACCTGAATGGATTTTGTGGATCTC.

Amino acid replacement: T1561S. An extra Q is inserted between the Q amino acids at position 2567 and 2568.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nnd-1 mutant flies exhibit significantly enhanced 24 hour long-term memory formation.

Nnd-1 hemizygous males have notched wings and thickened wing veins.

Nnd-1 mutant flies display a notched-wing phenotype.

At 18[o]C the eyes of Nnd-1 mutant adults are only slightly smaller and rougher than wild-type, moderate notching of the wing margin is also observed.

Male flies hemizygous for Nnd-1 show wings with minor margin nicking.

Mutants show gaps in the wing margin and reduced wing blade size.

Nl1N-ts1/Nnd-1 adults are produced at the permissive temperature.

Nnd-1 causes limited nicking in the margin of the adult wing.

Nnd-1/Y animals have extra joint material in the tarsal segment 4 but not in segment 3.

Nnd-1 mutants have notched wings.

Nnd-1 is a temperature-sensitive allele. In homozygous Nnd-1 mutants at 29[o]C, eyes are rough and reduced in size. They also exhibit extreme wing notching and thickened wing veins.

Nnd-1 mutants show significantly reduced mean character size for orbital bristles compared to Canton-S flies.

Wings show a cut phenotype, similar to that of ct53d.

Wing margin is scalloped. Wing veins are slightly thickened. Phenotype suggests loss of function effect in one context (wing margin formation) and gain of function effect in another (wing vein formation).

Homozygotes exhibit a weak N loss of function phenotype, having notched wings. This phenotype is fully penetrant but slightly variable.

Mutant flies show a weak loss of function phenotype and produce a slight excess of neural cells. Mutant cells in clones can influence their neighbours since fewer than expected wild type bristles are found along the mosaic borders.

Thickened wing veins with deltas and variably notched wing margins.

Thick wing veins, wing nicks along the apical, anterior and posterior margins.

Notching of the wing tip at 25oC.

The haplo-insufficient phenotype in the mesonotum is an increase in microchaetae density when Nnd-1 and Nnd-3 are in combination with N amorph alleles.

Wing notches in the adult. SerD/Nnd-1 transheterozygotes display a synergistic phenotype: loss of anterior and posterior wing margins and loss if distal wing blade tissue. Nnd-1/Df(3R)Bd males exhibit a significantly increased mutant wing phenotype compared to Nnd-1 alone.

Homozygous wing margins are excised. Wing phenotype can be enhanced in severity by vg and sca mutations. Phenotype can be suppressed by extra copies of sca+ and vg+.

Nnd-1/dxENU and Nnd-2/dxENU transheterozygous flies never reach adulthood, stage not determined. Enhance wing phenotype.

N/Nnd-1 heterozygotes are partially viable and relatively infertile.

Homozygotes have slightly small, rough eyes, extreme apical and marginal notches in the wing and thick wing veins at 29oC. At 25oC, the eyes are weakly rough, notches are seen in the wing and the wing veins are thick. At 18oC, the eyes are normal and the wings have weak nicks.

Similar to and phenotypically non-complementary with Nnd-3. Semilethal when heterozygous with dominant visible alleles of N : survivors are relatively infertile.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Nnd-1 has visible phenotype, enhanceable by Pcif1EY11352/CG11399[+]

Nnd-1 has visible | adult stage | temperature conditional phenotype, enhanceable by jigr1[+]/jigr12205

Nnd-1 has visible phenotype, enhanceable by RpL13AEP3614.A

Nnd-1 has visible phenotype, enhanceable by dom7

NOT Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

N[+]/Nnd-1 is a suppressor of visible | heat sensitive phenotype of HC2.hs

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Nnd-1 has wing margin phenotype, enhanceable by E-2g[+]/ebi2g1

Nnd-1 has wing vein phenotype, enhanceable by E-2g[+]/ebi2g1

Nnd-1 has wing margin phenotype, enhanceable by Past160-4

Nnd-1 has wing phenotype, enhanceable by Past1110-1

Nnd-1 has wing margin phenotype, enhanceable by Pcif1EY11352/CG11399[+]

Nnd-1 has eye | temperature conditional phenotype, enhanceable by jigr1[+]/jigr12205

Nnd-1 has wing | temperature conditional phenotype, enhanceable by jigr1[+]/jigr12205

Nnd-1 has wing blade phenotype, enhanceable by Nipped-A[+]/Nipped-A323

Nnd-1 has wing blade phenotype, enhanceable by Nipped-A[+]/Nipped-ANC96

Nnd-1 has wing blade phenotype, enhanceable by Nipped-A[+]/Nipped-ANC106

Nnd-1 has wing blade phenotype, enhanceable by Nipped-A[+]/Nipped-ANC186

Nnd-1 has wing blade phenotype, enhanceable by Nipped-A[+]/Nipped-ANC194

Nnd-1 has wing blade phenotype, enhanceable by domk08108

Nnd-1 has wing margin phenotype, enhanceable by dom7

Nnd-1 has tarsal segment & joint | ectopic phenotype, enhanceable by Su(dx)sp

Nnd-1 has tarsal segment 4 & joint | ectopic phenotype, enhanceable by Su(dx)sp

Nnd-1 has wing margin phenotype, enhanceable by Su(Tpl)[+]/Su(Tpl)10

Nnd-1 has wing margin phenotype, enhanceable by Su(Tpl)[+]/Su(Tpl)17

Nnd-1 has wing margin phenotype, enhanceable by Su(Tpl)[+]/Su(Tpl)S-192

Nnd-1 has wing margin phenotype, enhanceable by Su(Tpl)XS-706/Su(Tpl)[+]

Nnd-1 has wing margin phenotype, enhanceable by Su(Tpl)[+]/Su(Tpl)s1

Nnd-1 has wing phenotype, enhanceable by ebiE4

Nnd-1 has wing phenotype, enhanceable by Df(2R)Nipped-D341.1

Nnd-1 has wing phenotype, enhanceable by Df(2R)Nipped-E338

Nnd-1 has wing phenotype, enhanceable by Nipped-A222.3

Nnd-1 has wing phenotype, enhanceable by RpL3845-72

Nnd-1 has wing phenotype, enhanceable by Chie5.5

Nnd-1 has wing phenotype, enhanceable by vg1

Nnd-1 has wing phenotype, enhanceable by Su(H)16

Nnd-1 has wing phenotype, enhanceable by Su(H)[+]/Su(H)2

Nnd-1 has wing vein phenotype, enhanceable by Su(H)[+]/Su(H)2

Nnd-1 has wing phenotype, enhanceable by Su(H)[+]/Su(H)16

Nnd-1 has phenotype, enhanceable by sno71e3

Nnd-1 has wing phenotype, enhanceable by RpL19P

Nnd-1 has wing phenotype, enhanceable by Df(2R)ES1

Nnd-1 has wing phenotype, enhanceable by Df(2R)M60E

Nnd-1 has wing phenotype, enhanceable by RpL19ex1

Nnd-1 has wing phenotype, enhanceable by RpL19ex16

Nnd-1 has wing phenotype, enhanceable by RpL19ex51

Nnd-1 has wing phenotype, enhanceable by RpS5a2

Nnd-1 has wing phenotype, enhanceable by Df(3R)M86D

NOT Enhanced by
Statement
Reference

Nnd-1 has wing blade phenotype, non-enhanceable by Nipped-A[+]/Nipped-ANC105

Nnd-1 has wing blade phenotype, non-enhanceable by Ada2b[+]/Ada2bHD-5

Nnd-1 has wing blade phenotype, non-enhanceable by Ada2b[+]/Ada2b1

Nnd-1 has wing blade phenotype, non-enhanceable by Ada2b[+]/Ada2b2

Nnd-1 has wing blade phenotype, non-enhanceable by Ada2b[+]/Ada2b3412

Nnd-1 has phenotype, non-enhanceable by Su(H)8

Nnd-1 has phenotype, non-enhanceable by ap56f

Nnd-1 has phenotype, non-enhanceable by l(2)41Ae34

Suppressed by
Statement
Reference

Nnd-1 has wing phenotype, suppressible by Nipped-B407

Nnd-1 has wing phenotype, suppressible by Nipped-B292.1

Nnd-1 has wing phenotype, suppressible by Nle[+]/Nlek13714

Nnd-1 has wing vein phenotype, suppressible by Nle[+]/Nlek13714

Nnd-1 has wing phenotype, suppressible by Nle[+]/NleΔ8

Nnd-1 has wing vein phenotype, suppressible by Nle[+]/NleΔ8

Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

N[+]/Nnd-1 is a suppressor of wing vein | ectopic | heat sensitive phenotype of HC2.hs

N[+]/Nnd-1 is a suppressor of wing | heat sensitive phenotype of HC2.hs

Other
Additional Comments
Genetic Interactions
Statement
Reference

The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also carrying Nnd-1/Y.

The wing nicking phenotype caused by expression of Rbf280.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also carrying Nnd-1.

Heterozygosity for ebi2g1 enhances the notched wing margin and thickened wing vein phenotypes seen in Nnd-1 hemizygous males.

Nnd-1, Past160-4 double homozygous flies reach adulthood at a very low frequency, and die after 1 day. The wings are more severely notched compared to Nnd-1 mutant flies.

Nnd-1, Past1110-1 double homozygous flies reach adulthood at a very low frequency, and die after 1 day. The wings are more severely notched compared to Nnd-1 mutant flies.

The slightly reduced eye size and moderate wing margin nicking observed in the temperature-sensitive Nnd-1 homozygous adults at 18[o]C is enhanced by wgGla-1 heterozygosity: the ventral half of the adult eye is virtually completely lost and the wing notching phenotype is exacerbated too.

Wings from male flies hemizygous for Nnd-1 and heterozygous for RpL13AEP3614.A show a severe loss of wing material compared to Nnd-1/Y wings, whereas wings from RpL13AEP3614.A heterozygotes appear wild type.

Heterozygosity for Nipped-A323, Nipped-ANC96, Nipped-ANC106, Nipped-ANC186, Nipped-ANC194 or domk08108 further reduces the width of Nnd-1 wings.

Co-expression of Rtf1dsRNA.Sym.Scer\UAS under the control of Scer\GAL4hs.PB at 27[o]C significantly decreases the width of Nnd-1 wings relative to controls.

dom7 enhances the wing nicking phenotype of Nnd-1.

Nnd-1/Y, Su(dx)sp have extra, reversed polarity joints in tarsal segments three and four.

Wing notching in Nnd-1 mutants is enhanced by heterozygosity for Su(Tpl)s1, Su(Tpl)17, Su(Tpl)XS-706, Su(Tpl)10, or Su(Tpl)S-192.

The wing notching phenotype seen in hemizygous Nnd-1 animals is enhanced by the addition of ebiE4.

Wing vein thickening is enhanced by Su(dx)Scer\UAS.cCa driven by Scer\GAL4Bx-MS1096. Wing margin loss and wing size reduction are also evident.

Nnd-1/Y flies exhibit a minor wing nicking phenotype, and strongly enhance the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

l(2)41Ae7, ap56f and Su(H)8 have little or no effect on the Nnd-1 cut wing phenotype.

Nnd-1/Y; vgs2/vgs2 flies show notching of the wing. Nnd-1/Y; hi22/+ flies have severe notching of the wings.

Notched wing phenotype dominantly enhanced by Su(H)16.

Synergistic interaction of phenotype with that of sno71e3 mutation.

The Nnd-1 wing phenotype is enhanced by one copy of RpL19P, Df(2R)ES1 or Df(2R)M60E. The wing notching phenotype is also enhanced by RpL19ex1, RpL19ex16, RpL19ex51 RpS5a2 and Df(3R)M86D, M(2)2011 and RpS174.

Additive interactions with dx alleles.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (1)
Stocks (1)
Notes on Origin
Discoverer

A 'N[nd-1]' stock obtained from Spyros Artavanis-Tsakonas (termed 'SAT N[nd-1]') was found to contain a mixture of two different chromosomes: (i) the Nnd-1-dse mutation alone (at relatively low frequency); and (ii) the Nnd-1-dse mutation together with the 41 bp deletion within the N coding region described by Harding-Theobald et al. 2007 (FBrf0195930). A 'N[nd-1]' stock obtained from the Drosophila Stock Center (termed 'DSC N[nd-1]') contains only the chromosome containing both lesions. The Nnd-1-dse lesion appears to have been the original mutation, with 'N[nd-1]' flies originally containing this mutation alone. However, the chromosome containing both the Nnd-1-dse lesion and the 41 bp deletion within the N coding region has almost completely displaced the original chromosome in the 'SAT N[nd-1]' stock and has completely displaced it in the 'DSC N[nd-1]' stock.

Comments
Comments

FlyBase curator comment: The 'N[nd-1]' stock has been found to have acquired a second mutation in N over time (see FBrf0209477 for details). The Nnd-1-dse lesion (mutation in the Downstream Sequence Element of the consensus polyadenylation site) appears to have been the original mutation, with a 41 bp deletion within the N coding region occurring later. Data in FlyBase curated from individual papers using 'N[nd-1]' have been retained under the N[nd-1] allele record (FBal0012890), which represents the double mutant chromosome, unless it is clear that the chromosome being studied contains only the original lesion, in which case the data has been moved to the Nnd-1-dse allele record (FBal0282136). It is thus possible that some of the papers and curated data listed under N[nd-1] in FlyBase (especially information from older papers) describe experiments carried out using the Nnd-1-dse chromosome, but there is insufficient information to be sure. (date: 130225).

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (55)