Contains both the the 41 bp deletion within the N coding region described by Harding-Theobald et al. 2007 (FBrf0195930) and the mutation present in the progenitor Nnd-1-dse chromosome (T9408C substitution within the Downstream Sequence Element (DSE) of the consensus polyadenylation site).
41bp deletion at the 3' end of the 8th exon, within the intracellular domain. The deleted nucleotides, as determined by sequencing genomic DNA, are CGGTTCAGCGGGCTTGGACCTGAATGGATTTTGTGGATCTC.
Amino acid replacement: T1561S. An extra Q is inserted between the Q amino acids at position 2567 and 2568.
tarsal segment 4 & joint | ectopic
The slightly reduced eye size and moderate wing margin nicking observed in the temperature-sensitive Nnd-1 homozygous adults at 18[o]C is enhanced by wgGla-1 heterozygosity: the ventral half of the adult eye is virtually completely lost and the wing notching phenotype is exacerbated too.
Wings from male flies hemizygous for Nnd-1 and heterozygous for RpL13AEP3614.A show a severe loss of wing material compared to Nnd-1/Y wings, whereas wings from RpL13AEP3614.A heterozygotes appear wild type.
Synergistic interaction of phenotype with that of sno71e3 mutation.
The Nnd-1 wing phenotype is enhanced by one copy of RpL19P, Df(2R)ES1 or Df(2R)M60E. The wing notching phenotype is also enhanced by RpL19ex1, RpL19ex16, RpL19ex51 RpS5a2 and Df(3R)M86D, M(2)2011 and RpS174.
A 'N[nd-1]' stock obtained from Spyros Artavanis-Tsakonas (termed 'SAT N[nd-1]') was found to contain a mixture of two different chromosomes: (i) the Nnd-1-dse mutation alone (at relatively low frequency); and (ii) the Nnd-1-dse mutation together with the 41 bp deletion within the N coding region described by Harding-Theobald et al. 2007 (FBrf0195930). A 'N[nd-1]' stock obtained from the Drosophila Stock Center (termed 'DSC N[nd-1]') contains only the chromosome containing both lesions. The Nnd-1-dse lesion appears to have been the original mutation, with 'N[nd-1]' flies originally containing this mutation alone. However, the chromosome containing both the Nnd-1-dse lesion and the 41 bp deletion within the N coding region has almost completely displaced the original chromosome in the 'SAT N[nd-1]' stock and has completely displaced it in the 'DSC N[nd-1]' stock.