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General Information
Symbol
Dmel\Nipped-B407
Species
D. melanogaster
Name
FlyBase ID
FBal0098359
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
407
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nipped-B407/+ heterozygote adults are significantly lighter compared to wild-type, the effect is even more pronounced under limited nutrition conditions (which also leads to developmental delay comparable in length to controls).

Nipped-B407/+ females have smaller wings that contain fewer and smaller cells compared to wild-type although the size of cells in third instar larval wing disc (as measured by forward scattering) is not different from controls.

Nipped-B407/+ males show more modest reduction in courtship attempts toward an unreceptive female over time compared to wild-type controls, and thusly conditioned heterozygote mutant males do not display reduced courting toward a receptive female as is observed in wild-type, suggesting defects in learning and short-term memory. Nipped-B407/+ adult males also display morphological mushroom body defects with high frequency compared to controls.

Nipped-B407/+ adults display sex-dependent sleep defects: females sleep less than controls, both during the day and at night, although the total number of both daytime and nighttime sleep episodes is increased. The duration of each sleep episode however is significantly shorter. No significant change in the overall amount of sleep is observed in Nipped-B407/+ males although they display increased number of nighttime sleep episodes but these last slightly less long than in controls.

Both Nipped-B407/+ adult males and females show defects in circadian activity pattern - all of the females and a significant fraction of males are arrhythmic.

Nipped-B407/+ mutant adults do not display any seizure-like behaviors in response to either mechanical or thermal stress.

Heterozygous adults show a significant decrease in wing blade area compared to controls.

Chromosome cores and the synaptonemal complex fragment in germarial region 3 or stage 2 oocytes in Nipped-B407 heterozygotes, which is substantially earlier than in wild type.

Nipped-B407 heterozygotes do not show meiotic chromosome segregation defects.

Approximately 60% of metaphase nuclei in the brains of Nipped-B407/Nipped-B02047 and Nipped-B407 homozygous second instar larvae show precocious sister chromatid separation, in which the centromeric regions of the sister chromatids are somewhat detached from each other.

Recessive lethality acts at the L2-L3 molt.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Nipped-B407 has visible | dominant phenotype, suppressible by Mi-24/Mi-2[+]

Enhancer of
Statement
Reference

Nipped-B[+]/Nipped-B407 is an enhancer of visible phenotype of Ubxbx-34e, su(Hw)e2

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

Nipped-B407 has wing blade | female phenotype, suppressible by MycαTub84B.PBb

Nipped-B407 has wing blade phenotype, suppressible by Mi-24/Mi-2[+]

Enhancer of
Statement
Reference

Nipped-B[+]/Nipped-B407 is an enhancer of abdominal segment | adult stage phenotype of vtd80Fh-36

Nipped-B[+]/Nipped-B407 is an enhancer of wing phenotype of ctL32, su(Hw)e2

NOT Enhancer of
Statement
Reference

Nipped-B407 is a non-enhancer of phenotype of wgl-17

Nipped-B407 is a non-enhancer of phenotype of ap56f

Nipped-B407 is a non-enhancer of phenotype of mamg2.1

Nipped-B407 is a non-enhancer of phenotype of vg1

Nipped-B407 is a non-enhancer of phenotype of ct53d

Suppressor of
Statement
Reference

Nipped-B407 is a suppressor of sex comb | ectopic phenotype of Pc1

Nipped-B407 is a suppressor of sex comb | ectopic phenotype of Pc6

Nipped-B[+]/Nipped-B407 is a suppressor of eye phenotype of Nspl-1

Nipped-B407 is a suppressor of eye phenotype of Nspl-1

Nipped-B407 is a suppressor of wing phenotype of Nnd-1

NOT Suppressor of
Statement
Reference

Nipped-B407 is a non-suppressor of sex comb | ectopic phenotype of Psc1

Nipped-B407 is a non-suppressor of sex comb | ectopic phenotype of Sce1

Nipped-B407 is a non-suppressor of sex comb | ectopic phenotype of ph-d401

Nipped-B407 is a non-suppressor of phenotype of NAx-E2

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The small wing phenotype characteristic for Nipped-B407/+ adults is rescued by expression of MycαTub84B.PBb and leads to wings that are even larger than wild-type. Unlike in wild-type, expression of MycαTub84B.PBb in the Nipped-B407 heterozygous background does not lead to an increase in apoptosis (measured by TUNEL) in third instar larval wing discs.

Mi-24/+ suppresses the reduction in wing blade area seen in Nipped-B407/+ flies.

Nipped-B407/+ shows synthetic lethality with the P{Sym-UAS-Rad21}2 insertion line expressed under the control of Scer\GAL4Act5C.PI (this particular insertion line does not reduce viability when expressed using Scer\GAL4Act5C.PI at 25[o]C in an otherwise wild-type background).

Dominantly enhances the cut wing phenotype of ctL32, su(Hw)e2. Dominantly enhances the bithorax phenotype of Ubxbx-34e, su(Hw)e2. Shows slight dominant suppression of Nnd-1 wing nick phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments

Nipped-B407 homozygotes expressing Nipped-BChi.T:Avic\GFP-EGFP survive to pupariation at half the expected ratio, with developmental delays.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (12)