FlyBase Allele Report: Dmel\Mi-2[4]

General Information

Symbol

Dmel\Mi-2⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0094646

Feature type

allele

Associated gene

Dmel\Mi-2

Associated Insertion(s)

Carried in Construct

Also Known As

dMi-2⁴

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kehle et al., 1998)

Progenitor genotype

Cytology

Description

Insertion of 4bp after codon 398, resulting in a frameshift and a predicted premature termination within the first PHD finger domain.

(Kehle et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion

3L:19,887,268..19,887,269 [-]

Inserted_sequence:

GGCA

Comment:

Insertion of 4 bases (GGCA) after codon 398 causes a frameshift and leads to early translation termination.

(Kehle et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Mi-2^{Δ932-1158.UAS.Tag:FLAG}, Scer\GAL4^da.G32

(Fasulo et al., 2012)

lethal | recessive

(Fasulo et al., 2012, Kehle et al., 1998)

Phenotype Manifest In

egg | germline clone

(Kehle et al., 1998)

Detailed Description

Statement

Reference

The lethality caused by expression of Mi-2^{Δ932-1158.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32 is strongly enhanced by Mi-2⁴/+.

(Fasulo et al., 2012)

Heterozygotes have completely normal eyes.

(Marhold et al., 2004)

Homozygous female germline clones do not produce any eggs.

(Kehle et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Mi-2⁴ is an enhancer of abnormal neuroanatomy phenotype of ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of visible phenotype of HDAC1^3-10, ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of visible phenotype of HDAC1^5-5, ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of visible phenotype of HDAC1^1-3, ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of visible | dominant | homeotic phenotype of Pc⁴

(Kehle et al., 1998)

Suppressor of

Statement

Reference

Mi-2⁴/Mi-2[+] is a suppressor of visible | dominant phenotype of Nipped-B⁴⁰⁷

(Fasulo et al., 2012)

Other

Statement

Reference

MBD-like^EY04582, Mi-2⁴/Mi-2[+] has visible phenotype

(Marhold et al., 2004)

MBD-like^EY04582, Mi-2⁴ has visible phenotype

(Marhold et al., 2004)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Mi-2⁴ has phenotype, non-enhanceable by Alh^unspecified

(Perrin et al., 2003)

NOT suppressed by

Statement

Reference

Mi-2⁴ has phenotype, non-suppressible by Alh^unspecified

(Perrin et al., 2003)

Enhancer of

Statement

Reference

Mi-2⁴ is an enhancer of abdominal lateral pentascolopidial chordotonal organ lch5 phenotype of ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of wing sensillum | ectopic phenotype of HDAC1^3-10, ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of wing sensillum | ectopic phenotype of HDAC1^5-5, ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴ is an enhancer of larval abdominal lch5 neuron | increased number phenotype of ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of wing sensillum | ectopic phenotype of HDAC1^1-3, ttk^rM730

(Murawsky et al., 2001)

Mi-2⁴/Mi-2[+] is an enhancer of mesothoracic leg phenotype of Pc⁴

(Kehle et al., 1998)

Mi-2⁴/Mi-2[+] is an enhancer of metathoracic leg phenotype of Pc⁴

(Kehle et al., 1998)

Mi-2⁴/Mi-2[+] is an enhancer of sex comb | ectopic phenotype of Pc⁴

(Kehle et al., 1998)

Suppressor of

Statement

Reference

Mi-2⁴/Mi-2[+] is a suppressor of wing blade phenotype of Nipped-B⁴⁰⁷

(Fasulo et al., 2012)

Other

Statement

Reference

MBD-like^EY04582, Mi-2⁴/Mi-2[+] has eye phenotype

(Marhold et al., 2004)

MBD-like^EY04582, Mi-2⁴/Mi-2[+] has ommatidium phenotype

(Marhold et al., 2004)

MBD-like^EY04582, Mi-2⁴ has eye phenotype

(Marhold et al., 2004)

MBD-like^EY04582, Mi-2⁴ has ommatidium phenotype

(Marhold et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Mi-2⁴/+ suppresses the reduction in wing blade area seen in Nipped-B⁴⁰⁷/+ flies.

(Fasulo et al., 2012)

MBD-like^EY04582/Mi-2⁴ double heterozygotes have significantly smaller and rougher eyes than normal.

(Marhold et al., 2004)

The number of neurons associated with the pentascolopidial sensory organ in ttk^rM730 mutant embryos is significantly increased if they are also mutant for Mi-2⁴.

(Murawsky et al., 2001)

The formation of extra sex combs on the second and third legs in Pc⁴/+ flies is dominantly enhanced by Mi-2⁴.

(Kehle et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Mi-2⁴ is partially rescued by Scer\GAL4^da.G32/Mi-2^UAS.Tag:FLAG

(Fasulo et al., 2012)

Mi-2⁴ is partially rescued by Mi-2^arm.PK

(Kehle et al., 1998)

Comments

74% of Mi-2⁴ homozygotes are rescued to adulthood by expression of Mi-2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32.

(Fasulo et al., 2012)

The egg laying phenotype is rescued by Mi-2^arm.PK; normally shaped eggs are produced, but no embryos are obtained.

(Kehle et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26170

Mi-2⁴ red¹ e⁴/TM6B, Sb¹ Tb¹ ca¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mi-2⁴

(Lenz et al., 2021, Angulo et al., 2019, Kirilly et al., 2011)

Pha⁴

dMi-2⁴

(Fasulo et al., 2012, Read et al., 2005, Perrin et al., 2003, Murawsky et al., 2001, Kehle et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (9)

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