Nl1N-ts2 mutant lymph glands in third instar larvae do not exhibit a difference in lamellocyte differentiation and lobe dispersal in response to L. boulardi infection, as compared to controls.
Wound healing does not appear to be affected compared to controls in Nl1N-ts2/+ wing discs which have been fragmented (a 90[o] sector has been dissected out of the posterior compartment, leaving a 3/4 anterior fragment) and then implanted into wild-type females (analysed at 24 and 48 hours after implantation). However, the Nl1N-ts2/+ regenerating discs show significantly impaired proliferation compared to controls.
Stage 10 Nl1N-ts2 homozygous females, that have been shifted to a non-permissive temperature for less than 6 hours exhibit migration defects, despite these egg chambers already developing to stage 9, and follicle cell differentiation occurring, prior to the temperature shift (whereas wild-type controls exhibited no migration defects).
Adult flies show normal reactivity to electric shock and normal olfactory acuity (to methylcyclohexanol and octanol) after incubation at the restrictive temperature (30oC) for 2 days. Adult flies incubated for 2 days at the restrictive temperature show normal learning scores in a Pavlovian learning and memory assay. However, these flies show a significant reduction in one-day memory after spaced training compared to flies maintained at the permissive temperature.
When Nl1N-ts2 mutants are shifted to restrictive temperature between 104 and 128 h after egg laying (AEL) (8-32h after puparium formation (APF)), the L3 vein is broader, indicative of abnormal vein resolution. The campaniform sensillae (CS) are centered over the broader vein. When a transient temperature shift is applied at 60-80 h AEL, the L3 vein retains a wild-type width but its position is shifted anteriorly relative to the CS.
Shifting Nl1N-ts2 mutant larvae to the nonpermissive temperature in mid-late-L1 results in eye-antennal discs that are reduced in size.
Mutant larvae shifted to the non-permissive temperature (29oC) at the beginning of the L2 stage have abnormal wing discs. Mutant larvae shifted to the non-permissive temperature (29oC) at the beginning of the L3 stage have wing discs with a smaller wing pouch than normal. Adults derived from these larvae have notches at the distal tip of the wing.
Weak embryonic neurogenic phenotype at 29oC.
Lethal in combination with Df(1)N-8 at both 18oC and 29oC. 8% of homozygotes survive at 29oC, 97% survive at 18oC. Wings are normal in heterozygotes. Homozygotes are morphologically normal at 18oC. At 29oC, late pupae are head-eyeless, have a rough eye, stubby legs and irregular chaetae. Lethal in combination with Nl1N-ts1 at 29oC. 40% of Nl1N-ts2/NAx-tsl flies survive at 29oC and have an abruptex phenotype.
Similar to Nl1N-ts1 except for occasional survival of homozygotes to the pupal stage at 29oC and weaker expression of recessive visible defects in heterozygotes at this temperature.