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General Information
Symbol
Dmel\Nl1N-ts2
Species
D. melanogaster
Name
FlyBase ID
FBal0012888
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Nts2
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nl1N-ts2 mutant lymph glands in third instar larvae do not exhibit a difference in lamellocyte differentiation and lobe dispersal in response to L. boulardi infection, as compared to controls.

Wound healing does not appear to be affected compared to controls in Nl1N-ts2/+ wing discs which have been fragmented (a 90[o] sector has been dissected out of the posterior compartment, leaving a 3/4 anterior fragment) and then implanted into wild-type females (analysed at 24 and 48 hours after implantation). However, the Nl1N-ts2/+ regenerating discs show significantly impaired proliferation compared to controls.

Stage 10 Nl1N-ts2 homozygous females, that have been shifted to a non-permissive temperature for less than 6 hours exhibit migration defects, despite these egg chambers already developing to stage 9, and follicle cell differentiation occurring, prior to the temperature shift (whereas wild-type controls exhibited no migration defects).

Adult flies show normal reactivity to electric shock and normal olfactory acuity (to methylcyclohexanol and octanol) after incubation at the restrictive temperature (30oC) for 2 days. Adult flies incubated for 2 days at the restrictive temperature show normal learning scores in a Pavlovian learning and memory assay. However, these flies show a significant reduction in one-day memory after spaced training compared to flies maintained at the permissive temperature.

When Nl1N-ts2 mutants are shifted to restrictive temperature between 104 and 128 h after egg laying (AEL) (8-32h after puparium formation (APF)), the L3 vein is broader, indicative of abnormal vein resolution. The campaniform sensillae (CS) are centered over the broader vein. When a transient temperature shift is applied at 60-80 h AEL, the L3 vein retains a wild-type width but its position is shifted anteriorly relative to the CS.

Shifting Nl1N-ts2 mutant larvae to the nonpermissive temperature in mid-late-L1 results in eye-antennal discs that are reduced in size.

Mutant larvae shifted to the non-permissive temperature (29oC) at the beginning of the L2 stage have abnormal wing discs. Mutant larvae shifted to the non-permissive temperature (29oC) at the beginning of the L3 stage have wing discs with a smaller wing pouch than normal. Adults derived from these larvae have notches at the distal tip of the wing.

Weak embryonic neurogenic phenotype at 29oC.

Lethal in combination with Df(1)N-8 at both 18oC and 29oC. 8% of homozygotes survive at 29oC, 97% survive at 18oC. Wings are normal in heterozygotes. Homozygotes are morphologically normal at 18oC. At 29oC, late pupae are head-eyeless, have a rough eye, stubby legs and irregular chaetae. Lethal in combination with Nl1N-ts1 at 29oC. 40% of Nl1N-ts2/NAx-tsl flies survive at 29oC and have an abruptex phenotype.

Similar to Nl1N-ts1 except for occasional survival of homozygotes to the pupal stage at 29oC and weaker expression of recessive visible defects in heterozygotes at this temperature.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
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Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Shellenbarger.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (18)