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General Information
Symbol
Dmel\Nnd-2
Species
D. melanogaster
Name
FlyBase ID
FBal0012891
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
nd2
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Frameshift 14 residues before the C terminus, which leads to a truncated protein.

    Nnd-2 maps proximal to Nnd-1 at the 3' end of Notch and shows a deletion of one bp.

    Deletion of 1bp from codon 2690 causing a frameshift that changes the 14 carboxy terminal amino acid into a new sequence which is 23 amino acids in length.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Nnd-2 is a temperature-sensitive allele. In homozygous Nnd-1 mutants at 29[o]C, eyes are rough and reduced in size. They also show extreme wing notching and thickened wing veins.

    Nnd-2 mutants exhibit elevated levels of asymmetry and reduced mean character size relative to Canton-S flies for thoracic bristles.

    Nnd-2 mutants show significantly reduced mean character size for orbital bristles compared to Canton-S flies.

    Wing margin is scalloped. Wing veins are slightly thickened.

    At 25oC, nicks in wings. At 17oC displays Ax-type phenotypes. Reduces viability and increases penetrance of wing/haltere phenotypes of wg1. N, wg1 and arm4 triple mutants display, with about 10-20% penetrance, a perfect duplication of the notum charcateristic of wg mutations.

    Mutant flies have a variable mutant phenotype with a slightly increased or slightly decreased density of bristles in different regions of the thorax. Mutant cells in clones fail to prevent their wild type neighbours from becoming neural and more bristles than expected are of the wild type genotype.

    Thickened wing veins with deltas and variably notched wing margins.

    Nnd-2/Nnd-2 and Nnd-2/Nnd-1 flies resemble Nnd-1 homozygotes; Nnd-2/Nnd-3 heterozygotes are noncomplementary (Welshons). The Nnd-2 allele is temperature-sensitive; in homozygotes at 29oC, the eyes are small and rough (spl-like), wings have extreme notches, wing veins are thickened, tarsi are shortened and the mutants are semi-lethal as late pupae; at 25oC, the abnormalities are much less severe; at 18oC, the eyes are slightly spl-like, wings are nicked, wing veins are incomplete, some bristles are missing, and the mutants are semi-lethal as late pupae. Similar wing abnormalities in Nnd-1/Y; groE73/+ and Nnd-2/Y; groE73/+ males. RK1.

    Nnd-1/dxENU and Nnd-2/dxENU transheterozygous flies never reach adulthood, stage not determined. Enhance wing phenotype.

    Wing notching and thickened veins when homozygous or hemizygous. Temperature sensitive rough eye phenotype at 18oC.

    Homozygotes show late pupal semi-lethality at 18oC and 29oC. Homozygotes have small, rough eyes (like Nspl-1), extreme apical and marginal notches in the wing, thick wing veins and a shortened tarsus at 29oC. At 25oC, the Nspl-1-like eye phenotype, wing apical notching and thick wing vein phenotypes are seen. At 18oC, a weak Nspl-1-like eye phenotype, weak wing nicking, incomplete wing veins and missing bristles are seen.

    Similar to and phenotypically non-complementary with Nnd-3.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    Nnd-2 has phenotype, enhanceable by sno71e3

    Nnd-2 has eye phenotype, enhanceable by E(spl)[+]/E(spl)m8-HLH1

    Suppressed by
    Statement
    Reference

    Nnd-2 has wing phenotype, suppressible by Nle[+]/Nlek13714

    Nnd-2 has wing vein phenotype, suppressible by Nle[+]/Nlek13714

    Nnd-2 has wing phenotype, suppressible by Nle[+]/NleΔ8

    Nnd-2 has wing vein phenotype, suppressible by Nle[+]/NleΔ8

    Enhancer of
    Statement
    Reference

    Nnd-2 is an enhancer of phenotype of sno71e3

    Other
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Synergistic interaction of phenotype with that of sno71e3 mutation.

    Temperature sensitive rough eye phenotype at 18oC is enhanced by one copy of E(spl)1. When heterozygous with groE73 causes a severe wing material loss phenotype. Wing development is severely affected when heterozygous with mam10.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer

    R.M. Valencia,.

    Comments
    Comments

    Phenotype is sensitive to specific alleles of wg. Shows weak allele-specific interaction with wgl-15 and wgS107, and strong allele-specific interaction with wgl-12 and wgS84.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    References (19)