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General Information
Symbol
Dmel\Nnd-3
Species
D. melanogaster
Name
FlyBase ID
FBal0012892
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
nd3, fand, Nnd3, fano
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G3155989T

Amino acid change:

C105F | N-PA; C105F | N-PB

Reported amino acid change:

C105F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion producing a frameshift mutation.

Missense mutation in the intracellular domain.

Amino acid replacement: T1561S. Amino acid replacement: C105F. Mutation at position 1561 is conservative.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nnd-3 hemizygous adult males display nicks in the wing margin, vein deltas as well as vein thickening phenotype.

Nnd-3 mutant flies exhibit significantly enhanced 24 hour long-term memory formation.

Nnd-3 wings display big notches in the wing edges, enlarged veins, and prominent delta formation.

Mutants have a wing nicking phenotype.

Mutant flies have 98.66 +/- 1.86 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54).

Nnd-3 flies show excessive branching in the medulla. There is no significant difference in brain volume compared to wild-type flies.

Wing vein L3 is thickened.

Hemizygotes have nicked wings distally and thickened wing veins.

Wing veins are thickened.

Wing margin is scalloped. Wing veins are slightly thickened.

Flies show thickening of all longitudinal veins. Dorsal and ventral implants of Nnd-3 imaginal disc tissue into wild-type imaginal discs have enlarged veins which are all corrugated.

Mild wing nicking phenotype and affects the differentiation of marginal bristles.

Thickened wing veins with deltas and variably notched wing margins.

Individuals homozygous for Bpt1 and Nnd-3 exhibit no Bpt1 phenotype.

Thick wing veins with deltas, occasional apical wing nicks.

The haplo-insufficient phenotype in the mesonotum is an increase in microchaetae density when Nnd-1 and Nnd-3 are in combination with N amorph alleles.

Semilethal, adults exhibit wing notches. Wing phenotype can be enhanced in severity by vg and sca mutations.

Pronounced eye phenotype. Does not interact with dxENU.

Dp(2;Y)G44 males crossed to a stock carrying Nspl-1 produce sons that have straight wings or curly wings. Curly wings result from trisomy for the 44C--50B region: slightly suppress the mutant effect of Nnd-3.

At 29oC homozygotes have a weak thick wing vein phenotype with deltas at the wing vein tips. At 18oC and 25oC they have thick wing veins with deltas and apical notches in the wing.

Lethal when heterozygous with dominant visible alleles of N, semi-lethal when heterozygous with NCo or N60g11.

Hemizygotes and homozygotes have nicked wings and thickened wing veins. Lethal in combination with most N alleles. N264-103/Nnd-3 females survive when raised at 22oC but die at 29oC. One or several temperature sensitive periods (TSPs) for lethality occur during the embryonic, larval and prepupal-pupal stages (either a single TSP for lethality begins in the embryos stage and lasts into the pupal stage or there are several discrete TSPs).

Wings of both sexes notched at ends of L3 and L4 veins; wing veins enlarged and δ-like at tips. Mild mutant expression often limited to wing-vein effect. Mutant expression diminished at high temperature (Shellenbarger and Mohler, 1975). Heterozygotes show extremely weak dominance. Nnd-3/N almost completely lethal; survivors are sterile and have an exaggerated Notch phenotype. Nnd-3/fa closely resembles wild type. Heterozygotes with Nfa-g and spl are complementary; with nd and Nnd-2, heterozygotes are noncomplementary with a mild mutant expression of Nnd-3-like wings. Up to 5% of Nnd-3 males from aged cultures show hyper- and hypodeveloped external genitalia (Kroeger, 1960).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Nnd-3 has visible | adult stage phenotype, enhanceable by Kap-α3D93

Nnd-3 has visible | adult stage phenotype, enhanceable by Kap-α3D165

Nnd-3 has visible phenotype, enhanceable by klud00059/klu[+]

Nnd-3 has visible phenotype, enhanceable by sca[+]/scad09400

Suppressed by
Statement
Reference

Nnd-3 has visible phenotype, suppressible | partially by GPHRc06331/CG8090[+]

Nnd-3 has visible phenotype, suppressible by f06222f06222/f06222[+]

Nnd-3 has visible phenotype, suppressible | partially by dshhs.PA

Nnd-3 has visible phenotype, suppressible by pkpk-sple-13

NOT suppressed by
Statement
Reference

Nnd-3 has visible phenotype, non-suppressible by Klp64D[+]/Klp64Dk1

Nnd-3 has visible phenotype, non-suppressible by arm1/arm[+]

Suppressor of
Statement
Reference

Nnd-3/N[+] is a suppressor of visible | heat sensitive phenotype of HC2.hs

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Nnd-3 has wing margin phenotype, enhanceable by Kap-α3D93

Nnd-3 has wing margin phenotype, enhanceable by Kap-α3D165

Nnd-3 has wing margin phenotype, enhanceable by Kap-α3S033513

Nnd-3 has wing margin phenotype, enhanceable by Chc4

Nnd-3 has wing vein phenotype, enhanceable by Chc4

Nnd-3 has wing cell phenotype, enhanceable by Chc4

Nnd-3 has wing phenotype, enhanceable by klud00059/klu[+]

Nnd-3 has wing phenotype, enhanceable by sca[+]/scad09400

Nnd-3 has wing vein L3 phenotype, enhanceable by PsnW11/Psn[+]

Nnd-3 has wing vein L3 phenotype, enhanceable by Psn[+]/PsnW6

Nnd-3 has phenotype, enhanceable by bs03267

Nnd-3 has phenotype, enhanceable by dxS1M

Nnd-3 has phenotype, enhanceable by wgen11

Suppressed by
Statement
Reference

Nnd-3 has wing phenotype, suppressible | partially by GPHRc06331/CG8090[+]

Nnd-3 has wing phenotype, suppressible by f06222f06222/f06222[+]

Nnd-3 has microchaeta phenotype, suppressible | partially by dshhs.PA

Nnd-3 has wing vein phenotype, suppressible by pkpk-sple-13

Nnd-3 has wing phenotype, suppressible by pkpk-sple-13

Nnd-3 has wing vein phenotype, suppressible by Nle[+]/Nlek13714

Nnd-3 has wing phenotype, suppressible by Nle[+]/NleΔ8

Nnd-3 has wing vein phenotype, suppressible by Nle[+]/NleΔ8

Nnd-3 has wing phenotype, suppressible by Nle[+]/Nlek13714

NOT suppressed by
Statement
Reference

Nnd-3 has wing phenotype, non-suppressible by Klp64D[+]/Klp64Dk1

Nnd-3 has wing phenotype, non-suppressible by arm1/arm[+]

Enhancer of
Statement
Reference

Nnd-3 is an enhancer of wing vein phenotype of tkv1/tkv8

Suppressor of
Statement
Reference

Nnd-3/N[+] is a suppressor of wing vein | ectopic | heat sensitive phenotype of HC2.hs

Nnd-3/N[+] is a suppressor of wing | heat sensitive phenotype of HC2.hs

Other
Additional Comments
Genetic Interactions
Statement
Reference

One copy of Klp64Dk1 is unable to enhance the wing notching seen in Nnd-3 heterozygotes.

One copy of arm1 is unable to enhance the wing notching seen in Nnd-3 heterozygotes.

The wing margin notching phenotype observed in Nnd-3 hemizygous males is exacerbated by combination with a single copy of any of the following alleles: Kap-α3D93, Kap-α3D165 or Kap-α3S033513.

Chc4, Nnd-3 double hemizygous mutants display an enhanced wing phenotype compared to Nnd-3 single mutant wings. Loss of a great part of the wing surface and margin to extensive notches is observed, and loss of intervein tissue to vein overgrowth.

The number and severity of wing notching seen in Nnd-3 animals is dominantly enhanced by scad09400 or klud00059.

The Nnd-3 wing nicking phenotype is suppressed nearly to wild type by CG8090c06331/+.

The Nnd-3 wing nicking phenotype is suppressed to wild type by f06222f06222/+.

The number of microchaetae in Nnd-3 flies is increased to 119.33 +/- 1.54 by expression of dshhs.PA using a heat pulse.

CG6194P0997/CG6194P0997 enhances the loss of wing margin seen in Nnd-3/Y flies.

The wing phenotypes (thickened veins and wing-edge loss) of Nnd-3 flies are completely suppressed by pkpk-sple-13.

In double heterozygotes with PsnW6 or PsnW11, L3 is further thickened and wings are notched compared to Nnd-3 single heterozygotes.

The Nnd-3 wing phenotype is suppressed by HLHmβScer\UAS.cdCa expressed under the control of Scer\GAL432B.

The dpp loss of vein phenotype is epistatic in dpps4/dpps8 Nnd-3 double mutants, and the vein thickening characteristic of Nnd-3 is eliminated. The thickening of veins seen in tkv1/tkv8 flies is strongly enhanced in combination with Nnd-3.

Nnd-3/Y; vgs2/+ flies lack the distal portion of the ventral wing blade. The wing is greatly reduced in Nnd-3/Y ; vgs2/vgs2 flies, and marginal structures and veins are missing. These double homozygotes have low viability.

Synergistic interactions with dx alleles.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Bauer.

Comments
Comments

Mapped very close to N264-40 by genetic recombination.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (51)