FlyBase Allele Report: Dmel\E(spl)mβ-HLH[UAS.cdCa]

General Information

Symbol

Dmel\E(spl)mβ-HLH^UAS.cdCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of de Celis

FlyBase ID

FBal0052340

Feature type

allele

Associated gene

Dmel\E(spl)mβ-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)mβ}

Also Known As

UAS-E(spl)mβ, UAS-mβ

Key Links

Transgenic product class

wild_type

(de Celis et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(de Celis et al., 1996)

Mutagen

in vitro construct

(Ligoxygakis et al., 1998, de Celis, 1997, de Celis et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-E(spl)mβ}

Cytology

Description

Expression of E(spl)mβ-HLH is governed by UAS regulatory sequences.

(de Celis et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)mβ-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

some die during pupal stage, with Scer\GAL4^h-H10

(Ligoxygakis et al., 1998)

visible, with Scer\GAL4^32B

(Verheyen et al., 2001, Ligoxygakis et al., 1999)

visible, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^A9

(Xie et al., 2012)

visible, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^Bx-MS1096

(Baonza et al., 2000)

visible, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(de Celis et al., 1996)

macrochaeta, with Scer\GAL4^Bx-MS1096

(Fostier et al., 1998)

microchaeta, with Scer\GAL4^Bx-MS1096

(Fostier et al., 1998)

scutellar bristle, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

scutellar bristle, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

wing margin bristle, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

wing vein, with Scer\GAL4^32B

(Verheyen et al., 2001, Ligoxygakis et al., 1999)

wing vein, with Scer\GAL4^A9

(Xie et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Hasson et al., 2005, Baonza et al., 2000, Fostier et al., 1998)

wing vein L5, with Scer\GAL4^32B

(Verheyen et al., 2001)

Detailed Description

Statement

Reference

Overexpression of E(spl)mβ-HLH^{Scer\UAS.cdCa} under the control of Scer\GAL4^A9 results in wing veins that are slightly thicker than normal.

(Xie et al., 2012)

No extra neuroblasts are seen when E(spl)mβ-HLH^{Scer\UAS.cdCa} is expressed under the control of Scer\GAL4^wor.PA or Scer\GAL4^elav.PU.

(San-Juán and Baonza, 2011)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 causes a partial loss of wing veins.

(Hasson et al., 2005)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development.

(Kumar and Moses, 2001)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B results in a mild thinning of wing veins and shortening of vein L5.

(Verheyen et al., 2001)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 prevents the differentiation of all wing veins except L2 and the proximal regions of tL4 and L5.

(Baonza et al., 2000)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^ptc-559.1 causes a loss of most scutellar bristles.

(Apidianakis et al., 1999)

Flies expressing HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B show severe loss of wing veins. Loss of notum microchaetae and wing margin bristles is seen. Flies expressing HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^ap-md544 show complete loss of notum microchaetae and macrochaetae, except at the very anterior edge of the notum. Flies expressing HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^455.2 show severe loss of scutellar bristles. Flies expressing HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^h-1J3 show loss of notum macrochaetae.

(Ligoxygakis et al., 1999)

When expression is driven by Scer\GAL4^Bx-MS1096, flies show bristle loss and wing vein phenotypes.

(Fostier et al., 1998)

Eye discs patterning occurs almost normally in animals expressing two copies of HLHmβ^{Scer\UAS.cdCa} under the control of two copies of Scer\GAL4^h-H10. These animals die as pupae without differentiating adult structures. Flies expressing one copy of HLHmβ^{Scer\UAS.cdCa} under the control of one copy of Scer\GAL4^h-H10 are phenotypically wild-type.

(Ligoxygakis et al., 1998)

Scer\GAL4^c179-mediated expression in the wing causes deletion of most veins. Scer\GAL4^salm-459.2-mediated expression causes truncation of vein LIV. Expression of rho^{Scer\UAS.cdCa} rescues vein loss and results in thicker veins.

(de Celis et al., 1997)

When expression is governed by Scer\GAL4^ptc-559.1 in the wing disc, wing overgrowth and ectopic margin structures do not occur. The anterior cross vein is missing.

(de Celis et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 has visible phenotype, enhanceable by emc^UAS.cdCa, Scer\GAL4^Bx-MS1096

(Baonza et al., 2000)

NOT suppressed by

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has visible phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa is an enhancer of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^A9 is a suppressor of visible phenotype of Scer\GAL4^A9, uif^asterisk.UAS

(Xie et al., 2012)

Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa is a suppressor of visible phenotype of nmo^adk2/nmo^adk1

(Verheyen et al., 2001)

Scer\GAL4^salm-459.2/E(spl)mβ-HLH^UAS.cdCa is a suppressor of abnormal planar polarity | recessive phenotype of dsh¹

(Cooper and Bray, 1999)

Scer\GAL4^salm-459.2/E(spl)mβ-HLH^UAS.cdCa is a suppressor of visible | recessive phenotype of dsh¹

(Cooper and Bray, 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by emc^UAS.cdCa, Scer\GAL4^Bx-MS1096

(Baonza et al., 2000)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by Su(dx)^sp

(Fostier et al., 1998)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 has macrochaeta phenotype, enhanceable by Su(dx)^sp

(Fostier et al., 1998)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 has microchaeta phenotype, enhanceable by Su(dx)^sp

(Fostier et al., 1998)

NOT suppressed by

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has scutellar bristle phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of Scer\GAL4^Bx-MS1096, gro^UAS.cAa

(Hasson et al., 2005)

Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa is an enhancer of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^A9 is a suppressor of wing vein phenotype of Scer\GAL4^A9, uif^asterisk.UAS

(Xie et al., 2012)

Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa is a suppressor of wing vein | ectopic phenotype of nmo^adk2/nmo^adk1

(Verheyen et al., 2001)

Scer\GAL4^salm-459.2/E(spl)mβ-HLH^UAS.cdCa is a suppressor of ommatidium phenotype of dsh¹

(Cooper and Bray, 1999)

Scer\GAL4^salm-459.2/E(spl)mβ-HLH^UAS.cdCa is a suppressor of eye phenotype of dsh¹

(Cooper and Bray, 1999)

Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa is a suppressor of wing phenotype of N^nd-3

(Ligoxygakis et al., 1999)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^C-580 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^C-580, tkv^{Q253D.UAS.cLa}

(de Celis, 1997)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^bs-1348 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^bs-1348, tkv^{Q253D.UAS.cLa}

(de Celis, 1997)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, E(spl)mβ-HLH^UAS.cdCa is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of N^264-39

(Lu and Li, 2015)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, gro^DD.UAS

(Hasson et al., 2005)

E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, rl^Sem.cUa.UAS

(Hasson et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The thickened wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is almost completely suppressed by co-expression of E(spl)mβ-HLH^{Scer\UAS.cdCa}.

(Xie et al., 2012)

Coexpression of HLHmβ^{Scer\UAS.cdCa} and gro^Scer\UAS.cAa, under the control of Scer\GAL4^Bx-MS1096, leads to a greater loss of wing veins than expression of HLHmβ^{Scer\UAS.cdCa} alone. In contrast, coexpression of HLHmβ^{Scer\UAS.cdCa} and gro^DD.Scer\UAS, under the control of Scer\GAL4^Bx-MS1096, cannot suppress the wide wing vein phenotype seen when gro^DD.Scer\UAS is expressed alone. Coexpression of rl^Sem.Scer\UAS and HLHmβ^{Scer\UAS.cdCa}, under the control of Scer\GAL4^Bx-MS1096, results in wing vein thickening that is indistinguishable from expression of rl^Sem.Scer\UAS alone.

(Hasson et al., 2005)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B completely suppresses the extra wing vein phenotype of nmo^adk1/nmo^adk2 flies.

(Verheyen et al., 2001)

The loss of wing vein phenotype caused by expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 is enhanced by coexpression of emc^{Scer\UAS.cdCa}.

(Baonza et al., 2000)

Partially suppresses the eye tissue polarity phenotype of dsh¹ flies when expressed under the control of Scer\GAL4^salm-459.2.

(Cooper and Bray, 1999)

Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B enhances the scalloped wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the early third larval instar stage (72-84 hours after egg laying). Expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B has no effect on the nicked wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the mid third larval instar stage (96-108 hours after egg laying).

(Ligoxygakis et al., 1999)

The phenotype caused by expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 is strongly enhanced by Su(dx)^sp.

(Fostier et al., 1998)

Reduces but does not completely suppress the ectopic wing vein formation seen in tkv^{Q253D.Scer\UAS.cLa} Scer\GAL4^bs-1348 flies, when expressed using Scer\GAL4^bs-1348.

(de Celis, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26675

y¹ w^*; P{UAS-E(spl)mβ}48.1

Notes on Origin

Discoverer

Comments

The relative ability of UAS constructs of the E(spl) complex to induce wing vein deletion when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHmβ^{Scer\UAS.cdCa} = HLHmγ^Scer\UAS.cLa > E(spl)^Scer\UAS.cNa = HLHm7^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum microchaetae when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} >> HLHmγ^Scer\UAS.cLa = HLHmβ^{Scer\UAS.cdCa} = HLHm3^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of wing margin bristles when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHm7^{Scer\UAS.cdCa} > E(spl)^Scer\UAS.cNa > HLHmγ^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum macrochaetae when expressed under the control of Scer\GAL4^h-1J3 is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa} > HLHmγ^Scer\UAS.cLa = HLHm3^Scer\UAS.cLa.

(Ligoxygakis et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)mβ-HLH^{Scer\UAS.cdCa}

E(spl)mβ-HLH^UAS.cdCa

HLHmβ^{Scer\UAS.cdCa}

HLHmβ^UAS.cdCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Celis

(de Celis et al., 1996)

Secondary FlyBase IDs

References (28)

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