FlyBase Allele Report: Dmel\uif[asterisk.UAS]

UASt regulatory sequences drive expression of a polypeptide that lacks the last ten amino acids of at the C-terminus of the predicted full-length uif protein (amino acid 3548 to amino acid 3557), with two amino acid substitutions (N1567D and A3134T) and an addition of three extra amino acids (SGR) immediately immediately after amino acid 165 resulting from the insertion of a restriction enzyme Not I site in the coding sequence. Compared with wild-type uif, the protein encoded by the construct has an altered intracellular domain.

(Xie et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

uif

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^unspecified

(Xie et al., 2012)

visible, with Scer\GAL4^A9

(Xie et al., 2012)

visible, with Scer\GAL4^Bx-MS1096

(Xie et al., 2012)

visible, with Scer\GAL4^dpp.blk1

(Xie et al., 2012)

visible, with Scer\GAL4^en-e16E

(Xie et al., 2012)

visible, with Scer\GAL4^Eq

(Xie et al., 2012)

visible, with Scer\GAL4^GMR.PU

(Xie et al., 2012)

visible, with Scer\GAL4^pnr-MD237

(Xie et al., 2012)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Xie et al., 2012)

notal microchaeta, with Scer\GAL4^Eq

(Xie et al., 2012)

notal microchaeta, with Scer\GAL4^pnr-MD237

(Xie et al., 2012)

wing margin, with Scer\GAL4^dpp.blk1

(Xie et al., 2012)

wing margin, with Scer\GAL4^en-e16E

(Xie et al., 2012)

wing vein, with Scer\GAL4^A9

(Xie et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Xie et al., 2012)

Detailed Description

Statement

Reference

Ubiquitous expression of uif^{asterisk.Scer\UAS} causes a semi-lethal phenotype.

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^en-e16E results in significant loss of wing margin structures.

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 results in wing notches in the distal region.

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 leads to thickened wing veins.

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 leads to thickened wing veins.

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^Eq1 causes sensory mother cell (SOP) selection and formation defects including patches of bristle loss.

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 results in the nearly complete loss of bristles in the notum and scutellum

Expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in a rough and small eye phenotype.

(Xie et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^A9, uif^asterisk.UAS has visible phenotype, enhanceable by N¹/N[+]

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has visible phenotype, enhanceable by N[+]/N^55e11

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has visible phenotype, enhanceable by Delta^9P/Dl[+]

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has visible phenotype, enhanceable by kuz[+]/kuz^e29-4

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has visible phenotype, enhanceable by dx¹/dx[+]

(Xie et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^A9, uif^asterisk.UAS has visible phenotype, suppressible by E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^A9

(Xie et al., 2012)

Scer\GAL4^GMR.PU, uif^asterisk.UAS has visible phenotype, suppressible | partially by E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^GMR.PU

(Xie et al., 2012)

Enhancer of

Statement

Reference

uif^asterisk.UAS, Scer\GAL4^sca-109-68 is an enhancer of visible phenotype of Delta^DN.UAS, Scer\GAL4^sca-109-68

(Xie et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^A9, uif^asterisk.UAS has wing vein phenotype, enhanceable by N¹/N[+]

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has wing vein phenotype, enhanceable by N[+]/N^55e11

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has wing vein phenotype, enhanceable by Delta^9P/Dl[+]

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has wing vein phenotype, enhanceable by kuz[+]/kuz^e29-4

(Xie et al., 2012)

Scer\GAL4^A9, uif^asterisk.UAS has wing vein phenotype, enhanceable by dx¹/dx[+]

(Xie et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^A9, uif^asterisk.UAS has wing vein phenotype, suppressible by E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^A9

(Xie et al., 2012)

Scer\GAL4^GMR.PU, uif^asterisk.UAS has eye phenotype, suppressible | partially by E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^GMR.PU

(Xie et al., 2012)

Enhancer of

Statement

Reference

uif^asterisk.UAS, Scer\GAL4^sca-109-68 is an enhancer of scutellar bristle | increased number phenotype of Delta^DN.UAS, Scer\GAL4^sca-109-68

(Xie et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is enhanced by N¹/+.

The wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is enhanced by N^55e11/+.

The wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is enhanced by Dl^9P.

The wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is enhanced by kuz^e29-4.

The wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is enhanced by dx¹.

The scutellar bristle phenotype resulting from the expression of Dl^D.Scer\UAS under the control of Scer\GAL4^sca-109-68 is enhanced by co-expression of uif^{asterisk.Scer\UAS}.

The thickened wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is almost completely suppressed by co-expression of E(spl)mβ-HLH^{Scer\UAS.cdCa}.

The rough and small eye phenotype resulting from the expression of uif^{asterisk.Scer\UAS} is significantly alleviated by co-expression of E(spl)m7-HLH^{Scer\UAS.cdCa}.

(Xie et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

uif^{asterisk.Scer\UAS}

uif^asterisk.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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