UASt regulatory sequences drive expression of a polypeptide that lacks the last ten amino acids of at the C-terminus of the predicted full-length uif protein (amino acid 3548 to amino acid 3557), with two amino acid substitutions (N1567D and A3134T) and an addition of three extra amino acids (SGR) immediately immediately after amino acid 165 resulting from the insertion of a restriction enzyme Not I site in the coding sequence. Compared with wild-type uif, the protein encoded by the construct has an altered intracellular domain.