FlyBase Allele Report: Dmel\Kap-α3[D93]

General Information

Symbol

Dmel\Kap-α3^D93

Species

D. melanogaster

Name

FlyBase ID

FBal0160265

Feature type

allele

Associated gene

Dmel\Kap-α3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Mason et al., 2003)

Mutagen

Delta2-3 transposase

(Mason et al., 2003)

Progenitor genotype

Kap-α3^S033513

(Mason et al., 2003)

Cytology

Description

Imprecise excision of P{lacW}Kap-α3^S033513 has deleted 857bp from the 5' region of Kap-α3 including the first 20 codons of the Kap-α3 ORF.

(Mason et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates spinocerebellar ataxia 6

modeled by Hsap\CACNA1A^{UAS.αACT.Q70}

(Tsou et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal phototaxis | somatic clone

(Ting et al., 2007)

fertile

(Mason et al., 2003)

fertile | female (with Kap-α3^D165)

(Mason et al., 2003)

lethal (with Kap-α3^D165)

(Mason et al., 2003)

lethal - all die before end of larval stage

(Mason et al., 2003)

lethal - all die before end of larval stage (with Df(3R)by416)

(Mason et al., 2003)

lethal - all die before end of larval stage (with Df(3R)GB104)

(Mason et al., 2003)

lethal - all die before end of larval stage (with Kap-α3^17-7)

(Mason et al., 2003)

lethal - all die before end of larval stage (with Kap-α3^w73)

(Mason et al., 2003)

some die during larval stage

(Mason et al., 2003)

some die during larval stage (with Df(3R)by416)

(Mason et al., 2003)

some die during larval stage (with Df(3R)GB104)

(Mason et al., 2003)

some die during larval stage (with Kap-α3^17-7)

(Mason et al., 2003)

some die during larval stage (with Kap-α3^w73)

(Mason et al., 2003)

visible | somatic clone

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Somatic clones of Kap-α3^D93 homozygous cells either in the eye disc or salivary glands of third instar larvae display defects in N protein sub-cellular localization (enriched in cytoplasm) compared to controls.

(Sachan et al., 2013)

Mutant clones in the thorax result in multiple socket phenotypes.

(Mummery-Widmer et al., 2009)

Homozygous embryos produce wild-type cuticles.

Eggs cannot be recovered from females in which homozygous germ-line clones have been induced and which have been mated with either wild-type or heterozygous males.

(Chan et al., 2008)

A significant proportion of Kap-α3^D93 mosaic flies exhibit mutant R7 photoreceptor cells and do not phototax to visible light.

Kap-α3^D93 MARCM clone R7 axons terminate correctly in the M6 layer, although a significant proportion extend laterally into columns occupied by neighboring wildtype R7s.

(Ting et al., 2007)

Eyes homozygous for Kap-α3^D93 (somatic clones induced in the eye with Scer\FLP1^Scer\UAS.cDa; Scer\GAL4^ey.PH; twinspot and heterozygous cells killed by W^GMR.PG) are highly defective. Externally these eyes appear glassy, but ommatidial-like structures are visible although they are disorganized and not fully developed. Inter-ommatidial bristles are often missing. Sectioning reveals that photoreceptor cell rhabdomeres missing and the ommatidia were severely misshapen.

(Mason et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Kap-α3^D93 has lethal | larval stage phenotype, suppressible by Pen^-UTR.UASp/Scer\GAL4^αTub84B.PL

(Mason et al., 2003)

Kap-α3^D93 has lethal | larval stage phenotype, suppressible by Scer\GAL4^Act5C.PI/Pen^-UTR.UASp

(Mason et al., 2003)

Kap-α3^17-7/Kap-α3^D93 has lethal | larval stage phenotype, suppressible | partially by Pen^-UTR.UASp/Scer\GAL4^αTub84B.PL

(Mason et al., 2003)

Kap-α3^17-7/Kap-α3^D93 has lethal | larval stage phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Pen^-UTR.UASp

(Mason et al., 2003)

Kap-α3^17-7/Kap-α3^D93 has lethal | larval stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Kap-α1^UASp.cMa

(Mason et al., 2003)

Kap-α3^17-7/Kap-α3^D93 has lethal | larval stage phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Kap-α1^UASp.cMa

(Mason et al., 2003)

Kap-α3^D93 has lethal | larval stage phenotype, suppressible by Scer\GAL4^αTub84B.PL/Kap-α1^UASp.cMa

(Mason et al., 2003)

Kap-α3^D93 has lethal | larval stage phenotype, suppressible by Scer\GAL4^Act5C.PI/Kap-α1^UASp.cMa

(Mason et al., 2003)

Enhancer of

Statement

Reference

Kap-α3^D93 is an enhancer of visible | adult stage phenotype of N¹

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of visible | adult stage | male phenotype of dx¹⁵²

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of visible | adult stage phenotype of N^nd-3

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of visible | adult stage phenotype of Delta^5F

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of visible | adult stage phenotype of Ser^Bd-3

(Sachan et al., 2013)

Suppressor of

Statement

Reference

Kap-α3^D93/Kap-alpha3[+] is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

Kap-α3^D93 is a suppressor of visible | adult stage phenotype of N^Ax-16

(Sachan et al., 2013)

NOT Suppressor of

Statement

Reference

Kap-α3^D93/Kap-alpha3[+] is a non-suppressor of lethal - all die during pharate adult stage phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^sqh.PW

(Tsou et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Kap-α3^D93 has photoreceptor cell R7 | somatic clone phenotype, enhanceable by sev^V1

(Ting et al., 2007)

Suppressed by

Statement

Reference

Kap-α3^D93 has eye | somatic clone phenotype, suppressible by Scer\GAL4^ey.PH/Kap-α1^UASp.cMa

(Mason et al., 2003)

Kap-α3^D93 has ommatidium | somatic clone phenotype, suppressible by Scer\GAL4^ey.PH/Kap-α1^UASp.cMa

(Mason et al., 2003)

NOT suppressed by

Statement

Reference

Kap-α3^D93 has eye | somatic clone phenotype, non-suppressible by Pen^-UTR.UASp/Scer\GAL4^ey.PH

(Mason et al., 2003)

Kap-α3^D93 has ommatidium | somatic clone phenotype, non-suppressible by Pen^-UTR.UASp/Scer\GAL4^ey.PH

(Mason et al., 2003)

Kap-α3^D93 has rhabdomere | somatic clone phenotype, non-suppressible by Scer\GAL4^ey.PH/Kap-α1^UASp.cMa

(Mason et al., 2003)

Enhancer of

Statement

Reference

Kap-α3^D93 is an enhancer of wing vein | male phenotype of dx¹⁵²

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of wing margin phenotype of N¹

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of wing margin phenotype of N^nd-3

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of wing vein phenotype of Delta^5F

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of wing blade phenotype of Ser^Bd-3

(Sachan et al., 2013)

Kap-α3^D93 is an enhancer of wing margin phenotype of Ser^Bd-3

(Sachan et al., 2013)

Suppressor of

Statement

Reference

Kap-α3^D93/Kap-alpha3[+] is a suppressor of retina | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

Kap-α3^D93 is a suppressor of wing vein L5 phenotype of N^Ax-16

(Sachan et al., 2013)

Other

Statement

Reference

Kap-α3^D93, N^ICN.UAS, Scer\GAL4^Tub.PU has larval brain | somatic clone | third instar larval stage phenotype

(Sachan et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The wing margin notching phenotype observed in some N¹ heterozygous females and in N^nd-3 hemizygous males, the wing vein deltas and thickening observed in Dl^5F heterozygotes as well as the large nicks in the anterior wing margin seen in Ser^Bd-3 heterozygous flies are each exacerbated by combination with single copy of the Kap-α3^D93 allele.

The shortened longitudinal L5 phenotype characteristic for N^Ax-16 heterozygous females is suppressed by combination with a single copy of the Kap-α3^D93 allele.

The wing vein deltas and extra vein material observed in dx¹⁵² hemizygous adult males is exacerbated by combination with with a single copy of the Kap-α3^D93 allele. No wing defects are observed in adult females double heterozygote for dx¹⁵² and Kap-α3^D93.

The nuclear translocation of Notch-ICD (intracellular part of the N receptor) in somatic MARCM clones expressing N^ICN.Scer\UAS under the control of Scer\GAL4^tub.PU in third instar larval brain is blocked when the clones are also homozygous mutant for Kap-α3^D93.

(Sachan et al., 2013)

Removing neighboring R7 photoreceptor cells through a sev^V1 background greatly increases the tendency of Kap-α3^D93 mutant R7s to invade adjacent targets. Approximately 84.3% of isolated Kap-α3^D93 R7 terminals extend laterally into neighboring columns (as compared to 23% for Kap-α3^D93 mutants in a wild-type background), with 22% of these lateral extensions spanning several columns.

(Ting et al., 2007)

Kap-α1^{Scer\UAS.P\T.cMa} or Pen^{-UTR.Scer\UAS.P\T} driven by Scer\GAL4^Act5C.PI or Scer\GAL4^αTub84B.PL can weakly suppress the first instar larval lethality of Kap-α3^D93 homozygotes or Kap-α3^D93/Kap-α3^17-7 animals. Many larvae survive to second or early third instar, and occasionally animals survive to pupation (or even pharate adult stage in the case of the heterozygotes rescued with Pen^{-UTR.Scer\UAS.P\T}). Kap-α1^{Scer\UAS.P\T.cMa}; Scer\GAL4^ey.PH weakly suppresses the eye phenotype due to Kap-α3^D93 somatic clones: Externally, individual ommatidia appear more distinct than in non-rescued animals. Sectioning reveals that rhabdomere formation is not rescued, and ommatidia remain disorganized. Pen^{-UTR.Scer\UAS.P\T}; Scer\GAL4^ey.PH fails to suppress the eye phenotypes due to Kap-α3^D93 somatic clones.

(Mason et al., 2003)

Xenogenetic Interactions

Statement

Reference

Kap-α3^D93/+ does not suppress lethality in flies with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^sqh.PW. Co-expression of Kap-α3^D93/+ suppresses the progressive retinal degeneration seen in fly eyes with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^GMR.PU.

(Tsou et al., 2015)

Complementation and Rescue Data

Rescued by

Kap-α3^17-7/Kap-α3^D93 is rescued by Scer\GAL4^αTub84B.PL/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Kap-α3^w73/Kap-α3^D93 is rescued by Scer\GAL4^αTub84B.PL/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Partially rescued by

Df(3R)GB104/Kap-α3^D93 is partially rescued by Scer\GAL4^αTub84B.PL/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Kap-α3^D93/Kap-α3^D165 is partially rescued by Scer\GAL4^αTub84B.PL/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Kap-α3^D93 is partially rescued by Scer\GAL4^ey.PH/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Kap-α3^D93 is partially rescued by Scer\GAL4^αTub84B.PL/Kap-α3^-UTR.UASp

(Mason et al., 2003)

Comments

Kap-α3^{-UTR.Scer\UAS.P\T}; Scer\GAL4^αTub84B.PL weakly rescues the larval lethality of Kap-α3^D93/Kap-α3^D93, Kap-α3^D93/Kap-α3^D165 (around 40% still die as larvae, but about 50% die as pupae and about 10% as pharate adults) and Kap-α3^D93/Df(3R)GB104 (about 30% survive to pupate before dying). Kap-α3^{-UTR.Scer\UAS.P\T}; Scer\GAL4^αTub84B.PL rescues Kap-α3^D93/Kap-α3^17-7 and Kap-α3^D93/Kap-α3^w73 to fully viable, fertile adults. Kap-α3^{-UTR.Scer\UAS.P\T}; Scer\GAL4^ey.PH partially rescues the eye phenotypes due to Kap-α3^D93 somatic clones: Externally, individual ommatidia are much more distinct than without rescue, but most interommatidial bristles are missing. Sectioning reveals ommatidia to be somewhat disorganised and misshapen, with incorrect numbers of photoreceptors per ommatidium. Unlike in the non-rescued animals, rhabdomeres are present.

(Mason et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25397

w^*; Kap-α3^D93/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Kap-alpha3^D93

(Tsou et al., 2015)

Kap-α3^D93

(Mummery-Widmer et al., 2009)

imp α3^D93

(Sachan et al., 2013)

importin-α3^D93

(Chan et al., 2008)

impα3^D93

(Ting et al., 2007)

α3^D93

(Mason et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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