FlyBase Allele Report: Dmel\Kap-α1[UASp.cMa]

General Information

Symbol

Dmel\Kap-α1^UASp.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0137476

Feature type

allele

Associated gene

Dmel\Kap-α1

Associated Insertion(s)

Carried in Construct

P{UASp-Kap-α1.M}

Also Known As

UASp Importin α1

Key Links

Transgenic product class

wild_type

(Mason et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Mason et al., 2003)

Mutagen

in vitro construct

(Mason et al., 2003, Mason et al., 2002)

Progenitor genotype

Carried in construct

P{UASp-Kap-α1.M}

Cytology

Description

Expression of the Kap-α1 ORF is under the control of UASp regulatory sequences. 1 nucleotide of Kap-α1 3' UTR sequence is also present in the construct.

(Mason et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Kap-α1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Kap-α1^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^en.PU does not perturb the N protein localization.

(Sachan et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL/Kap-α1^UASp.cMa is a suppressor | partially of lethal | larval stage phenotype of Kap-α3^17-7/Kap-α3^D93

(Mason et al., 2003)

Scer\GAL4^Act5C.PI/Kap-α1^UASp.cMa is a suppressor | partially of lethal | larval stage phenotype of Kap-α3^17-7/Kap-α3^D93

(Mason et al., 2003)

Scer\GAL4^αTub84B.PL/Kap-α1^UASp.cMa is a suppressor of lethal | larval stage phenotype of Kap-α3^D93

(Mason et al., 2003)

Scer\GAL4^Act5C.PI/Kap-α1^UASp.cMa is a suppressor of lethal | larval stage phenotype of Kap-α3^D93

(Mason et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UASp.cMa is a non-suppressor of increased mortality during development | maternal effect phenotype of Fs(2)Ket^RE34, Pen^D14

(Virágh et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ey.PH/Kap-α1^UASp.cMa is a suppressor of eye | somatic clone phenotype of Kap-α3^D93

(Mason et al., 2003)

Scer\GAL4^ey.PH/Kap-α1^UASp.cMa is a suppressor of ommatidium | somatic clone phenotype of Kap-α3^D93

(Mason et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey.PH/Kap-α1^UASp.cMa is a non-suppressor of rhabdomere | somatic clone phenotype of Kap-α3^D93

(Mason et al., 2003)

Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UASp.cMa is a non-suppressor of nurse cell ring canal phenotype of Pen^D14

(Mason et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Kap-α1^{Scer\UAS.P\T.cMa} or Pen^{-UTR.Scer\UAS.P\T} driven by Scer\GAL4^Act5C.PI or Scer\GAL4^αTub84B.PL can weakly suppress the first instar larval lethality of Kap-α3^D93 homozygotes or Kap-α3^D93/Kap-α3^17-7 animals. Many larvae survive to second or early third instar, and occasionally animals survive to pupation (or even pharate adult stage in the case of the heterozygotes rescued with Pen^{-UTR.Scer\UAS.P\T}). Kap-α1^{Scer\UAS.P\T.cMa}; Scer\GAL4^ey.PH weakly to suppresses the eye phenotype due to Kap-α3^D93 somatic clones: Externally, individual ommatidia appear more distinct than in non-rescued animals. Sectioning reveals that rhabdomere formation is not rescued, and ommatidia remain disorganized.

(Mason et al., 2003)

Expression of Kap-α3^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the sterility of Pen^D14 females.

(Mason et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Act5C.PI/Kap-α1^UASp.cMa partially rescues Df(3L)α1S1

(Ratan et al., 2008)

Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UASp.cMa partially rescues Df(3L)α1S1

(Ratan et al., 2008)

Comments

Expression of Kap-α1^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^Act5C.PI efficiently restores fertility in Df(3L)α1S1 males, but only moderately rescues the fertility of Df(3L)α1S1 females.

Expression of Kap-α1^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} efficiently restores fertility in Df(3L)α1S1 females and the fecundity of these animals is nearly normal.

(Ratan et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Kap-α1^{Scer\UAS.P\T.cMa}

Kap-α1^UASp.cMa

Name Synonyms

Secondary FlyBase IDs

References (5)

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