neuroblast | supernumerary & larval brain | somatic clone, with Scer\GAL4Act5C.PI
The co-expression of MycKK103869 and NICN.Scer\UAS under the control of Scer\GAL4E(spl)m6-BFM.PF leads to significantly decreased numbers of abdominal lateral, dorsal and ventral adult muscle precursor cells in third instar larvae, as compared to controls.
The nuclear translocation of Notch-ICD (intracellular part of the N receptor) in somatic MARCM clones expressing NICN.Scer\UAS under the control of Scer\GAL4tub.PU in third instar larval brain is blocked when the clones are also homozygous mutant for Kap-α3D93.
The adult eye defects - irregularities in size and spacing of ommatidia and aberrant numbers of interommatidial bristles - as well as decreased eclosion rate and reduced adult life-span observed in flies expressing NICN.Scer\UAS under the control of Scer\GAL4ey.PU is exacerbated further by co-expression of Kap-α3Scer\UAS.T:Ivir\HA1. The neoplastic overgrowth of both eye and wing discs induced by NICN.Scer\UAS expression (controlled by either the Scer\GAL4ey.PU or the Scer\GAL4ap.PU driver, respectively) is also strongly enhanced by Kap-α3Scer\UAS.T:Ivir\HA1 co-expression.
Expression of NICN.Scer\UAS under the control of Scer\GAL4bab1-Gal4 (limited to the adult stages using Scer\GAL80ts.αTub84B) significantly suppresses the germline stem cell and cap cell loss seen in InR339/InRE19 mutant week old females.
Co-expression of NICN.Scer\UAS results in enlarged wing discs containing a larger field (30-40% of disc surface area) of differentiating ectopic eye precursors compared to expression of eyScer\UAS.cHa alone under the control of Scer\GAL4ap-md544.
Expression of NICN.Scer\UAS under the control of Scer\GAL4arm.PS in pcx3 embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 44% of embryos are rescued, 48% are partially rescued and 8% are not rescued. The endoplasmic reticulum defects are also rescued.
The co-expression of panΔN.Scer\UAS suppresses the enlarged eye in adults and the increased proliferation and enlarged morphogenetic furrow in third instar larva eye discs expressing NICN.Scer\UAS driven by Scer\GAL4ey.PS.
Apcc00746 or the co-expression of either wgd08266 or armS10.Scer\UAS.T:Hsap\MYC enhance the eye defects in adults expressing NICN.Scer\UAS driven by Scer\GAL4ey.PS, with associated wrinkling and distortion in third instar larva eye discs, but no obvious differentiation defects are visible in these discs.
Homozygous mutant auxI670K eye discs that also express NICN.Scer\UAS (under the control of Scer\GAL4GMR.PF) exhibit a reduction in the number of elav-positive cells, indicating that N is epistatic to aux.
Lsi strongly suppresses the overgrowth of the ventral part of the eye disc in animals expressing NICN.Scer\UAS under the control of Scer\GAL4dpp.blk1, but has little effect on dorsal outgrowth of the eye discs in these animals.
Expression of AntpScer\UAS.cBa and NICN.Scer\UAS driven by Scer\GAL4ey.PH is lethal. About 1/4 of those few escapers that do survive are found to have ectopic wings on their heads that usually consisted of dorsal and ventral wing blades bordered by bristles of the wing margin, but lacking wing veins. About 17% of these flies showed ectopic leg structures induced by secondary transformation of the ectopic antennal tissue into leg structures, an additional 10% showed a transformation of the original antenna to leg structures.
Co-expression of Hsap\NUMBScer\UAS.PTBS-PRRL increases the number of neuroepithelial cells but does not suppress the inhibition of neuronal differentiation seen in clones with expression of NICN.Scer\UAS in third instar larval neuroblasts.