DlRevF10, SerRX82 transheterozygotes have normal sized eyes, but eygM3-12; DlRevF10, SerRX82 triple heterozygous flies have small eyes. HE31/+ partially restores eye development in eyg1/eygM3-12 flies, as does NICN.Scer\UAS; Scer\GAL4dpp.blk1 or Su(H)Scer\UAS.cNa; Scer\GAL4dpp.blk1. Head development in eygM3-12 homozygous flies is partially rescued by HE31/+: the resulting flies appear to have a complete head apart from lacking eyes. Head development in these flies is also partially rescued by NICN.Scer\UAS; Scer\GAL4ey.PH : although only 6% of these animals survive to the pharate stage, those that do have heads but usually lack eyes. The eye-antennal discs of eygM3-12/eygM3-12; NICN.Scer\UAS; Scer\GAL4ey.PH larvae have a highly reduced eye field with no photoreceptor differentiation, but an extra eye field with photoreceptor duplication is present dorsal to the endogenous eye field in about a third of cases. In contrast the antennal field of these discs is always present and is sometimes duplicated or triplicated. upd1Scer\UAS.cZa; Scer\GAL4dpp.blk1 completely restores eye development in eyg1/eygM3-12 flies.
Expression of eygScer\UAS.lune under the control of Scer\GAL4CD rescues the loss of the eye-antennal disc and headless phenotype of the eygM3-12 mutant. In some cases, duplication of the larval eye-antennal disc and adult antenna occurs. The notum of these animals remains grooved.
Expression of eygScer\UAS.lune under the control of Scer\GAL4EX, Scer\GAL4F1, or Scer\GAL4XS partially rescues the eygM3-12 mutant. In all cases a small head with antennae but without eyes develops. The rescue efficiency of Scer\GAL4XS (76%) and Scer\GAL4EX (60%) are both much higher than Scer\GAL4F1 (7%).
Expression of eygScer\UAS.lune under the combined control of Scer\GAL4EX and Scer\GAL4XS partially rescues the eygM3-12 mutant to a slightly higher percentage (85%) than either driver alone. In this case the same level of rescue is observed, with flies having a small head with antennae but no eyes.
Expression of eygScer\UAS.lune under the transient control of Scer\GAL4CD during stage L2 (expression restricted by Scer\GAL80ts.αTub84B throughout other stages of development) significantly rescues the eygM3-12 eye mutant phenotype. Earlier and later expression are not sufficient to rescue the eye development.
Expression of eygmPD.Scer\UAS, under the control of Scer\GAL4eyg-22-2, partially rescues the eye phenotype of eyg22-2/eygM3-12 mutants, but has a dominant-negative effect on the thorax phenotype. Expression of eygΔHD.Scer\UAS in a eyg22-2/eygM3-12 background leads to partial rescue of the mutant eye and mutant thorax phenotypes. Expression of the eygmHD.Scer\UAS transgene in a eyg22-2/eygM3-12 background leads to the full rescue of eyes in 21% of mutants. The mutant thorax is fully rescued, although there are still many extra macrochaetes at lateral regions. Coexpression of the transgenes eygmHD.Scer\UAS and eygmPD.Scer\UAS in a eyg22-2/eygM3-12 background fully rescues the mutant eye and thorax phenotypes.