FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{lacW}
Open Close
General Information
Symbol
P{lacW}
FlyBase ID
FBtp0000204
Feature type
transgenic_transposable_element
Component Allele(s)
Size
10.691
Expression Data
Associated insertion(s)
7167 available
Molecular map
Description and Uses
Description

P{lacW} is an enhancer trap construct that contains at its 5' end the weak P\T promoter fused upstream of sequence encoding the lacZ reporter enzyme (the lacZ sequence is fused in-frame to the P\T coding sequence at approximately the same position as in P{A92}). The expressed lacZ reporter protein is nuclear, independent of insertion site, probably due the fusion to P\T coding sequence, since native P\T protein is nuclear.

(Bier et al., 1989)

A diagram of the P{lacW} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

(FlyBase, 1992-)
Construct components
Component
Use(s)
Element type
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Marker(s)
w+mC
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

10.691

Left end

P5\'

(Bier, 1995.10.13)
Right end

P3\'

(Bier, 1995.10.13)
 
Segments
Num
Orientation
Symbol
1
+
P\T::lacZ(P5',Bg2)
2
+
001094
3
+
w+mC4.1(Sp)
4
+
001095
5
-
pBR322ori/amp(-Pst)
6
+
001096
7
+
6.1_P3'(X1,P3')
Reference
(Bier et al., 1989)
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{lacW}
(Bier et al., 1989)
descendant(s)
P{3'lacW.2Bab-10}
(Gonzy-Treboul et al., 1995)
P{3'lacW.3HP}
(Ring et al., 2000)
P{3'lacW.5R5.24}
(Szuplewski et al., 2003)
P{3'lacW}
(FlyBase, 1992-)
P{5'lacW.5R1.5}
(Szuplewski et al., 2003)
P{5'lacW.5R7.2}
(Szuplewski et al., 2003)
P{5'lacW}
(FlyBase, 1992-)
P{?'lacW}
(FlyBase, 1992-)
P{?lacW}
(FlyBase, 1992-)
P{lacW.ph+2}
(Fauvarque et al., 1995)
P{lacW.ph+3}
(Fauvarque et al., 1995)
P{lacW-Δ2.R}
(Ruhf et al., 2001)
P{lacW-Δ3.R}
(Ruhf et al., 2001)
P{lacW-Δ4}
(Roman et al., 2000)
P{lacW-Δ5M}
(Rodrigues et al., 1995)
P{lacW-Δ6.R}
(Ruhf et al., 2001)
P{lacW-Δ7.R}
(Ruhf et al., 2001)
P{lacW-Δ7id}
(Beck et al., 2004)
P{lacW-Δ9.R}
(Ruhf et al., 2001)
P{lacW-Δ10.R}
(Ruhf et al., 2001)
P{lacW-Δ11.R}
(Ruhf et al., 2001)
P{lacW-Δ12.M}
(Meyer et al., 1998)
P{lacW-Δ12.R}
(Ruhf et al., 2001)
P{lacW-Δ12}
(Rodrigues et al., 1995)
P{lacW-Δ13.R}
(Ruhf et al., 2001)
P{lacW-Δ15.R}
(Ruhf et al., 2001)
P{lacW-Δ16.R}
(Ruhf et al., 2001)
P{lacW-Δ17.R}
(Ruhf et al., 2001)
P{lacW-Δ18.R}
(Ruhf et al., 2001)
P{lacW-Δ80BL}
(Gonzy et al., 2002)
P{lacW-Δ.5HP}
(Ring et al., 2000)
P{lacW-Δexc2}
(Taillebourg and Dura, 2000)
P{lacW-Δexc8}
(Taillebourg and Dura, 2000)
P{lacW-Δix1}
(Jackson and Berg, 2002)
P{lacW-Δix2}
(Jackson and Berg, 2002)
P{lacW-Δix4}
(Jackson and Berg, 2002)
P{lacW-ΔR18}
(Hartenstein et al., 1997)
P{lacW-ΔR27}
(Hartenstein et al., 1997)
P{lacW-ΔR33}
(Hartenstein et al., 1997)
P{lacW-ΔR38}
(Hartenstein et al., 1997)
P{lacW-ΔS089302rv1}
(Kuhfittig et al., 2001)
P{lacW-ΔS089302rv6}
(Kuhfittig et al., 2001)
P{lacW-ΔS089302rv7}
(Kuhfittig et al., 2001)
P{lacW-ΔS089302rv9}
(Kuhfittig et al., 2001)
Comments

construct_comment: Restriction sites useful for plasmid rescue: (right) EcoRI, SacII; (left) XbaI, BglII, PstI, BamHI.

(Bier et al., 1989)
Stocks (2,167)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (145)