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General Information
Symbol
P{lacW}
FlyBase ID
FBtp0000204
Feature type
transgenic_transposable_element
Component Allele(s)
Size
10.691
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description

P{lacW} is an enhancer trap construct that contains at its 5' end the weak P\T promoter fused upstream of sequence encoding the lacZ reporter enzyme (the lacZ sequence is fused in-frame to the P\T coding sequence at approximately the same position as in P{A92}). The expressed lacZ reporter protein is nuclear, independent of insertion site, probably due the fusion to P\T coding sequence, since native P\T protein is nuclear.

A diagram of the P{lacW} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

Component allele w+mC
Product class / Tool use(s)
Regulatory region(s)
Description

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size

10.691

Left end
Right end
 
Features
Component Alleles
Allele
Reference(s)
Molecular data

4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

construct_comment: Restriction sites useful for plasmid rescue: (right) EcoRI, SacII; (left) XbaI, BglII, PstI, BamHI.

Stocks (2,253)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (143)