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General Information
Symbol
Dmel\eyUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Halder
FlyBase ID
FBal0045627
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ey, UAS-eyeless
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a full length embryonic ey cDNA is governed by UAS.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

antenna & eye | ectopic, with Scer\GAL4upd1-E132

antennal disc & eye | ectopic, with Scer\GAL4dpp.blk1

eye photoreceptor cell & dorsal mesothoracic disc | ectopic, with Scer\GAL4dpp.blk1

eye photoreceptor cell & ventral thoracic disc | ectopic, with Scer\GAL4dpp.blk1

eye photoreceptor cell | ectopic & antennal disc, with Scer\GAL4dpp.blk1

haltere & eye | ectopic, with Scer\GAL4upd1-E132

haltere & ommatidium | ectopic, with Scer\GAL4dpp.blk1

leg & eye | ectopic, with Scer\GAL4dpp.blk1

leg & eye | ectopic, with Scer\GAL4upd1-E132

proboscis & ommatidium | ectopic, with Scer\GAL4dpp.blk1

ventral thoracic disc & eye | ectopic, with Scer\GAL4dpp.blk1

wing & eye | ectopic, with Scer\GAL4MS941

wing & eye | ectopic, with Scer\GAL4upd1-E132

Detailed Description
Statement
Reference

Expression of eyUAS.cHa under the control of Scer\GAL4elav-C155 does not increase mitotic proliferation in the ventral nerve cord during late embryogenesis, compared to controls.

The expression of eyScer\UAS.cHa under the control of Scer\GAL4Bx-MS1096 induces ectopic photoreceptor formation in a small proportion of third instar larval wing disc cells, as compared to controls.

While Scer\GAL4Act5C.PI>eyScer\UAS.cHa expressing clones are significantly larger than wild-type controls located in the notum region of the wing disc, no significant difference is observed for clones located in the pouch or hinge region.

Expression of eyScer\UAS.cHa under the control of Scer\GAL430A induces ectopic eye on the wing hinge.

Co-expression of eyΔPD.cWa.Scer\UAS significantly decreases the ability of Scer\GAL430A>eyScer\UAS.cHa to induce ectopic eye formation.

Co-expression of eyScer\UAS.cTa significantly decreases the ability of Scer\GAL430A>eyScer\UAS.cHa to induce ectopic eye formation.

Expression of eyScer\UAS.cHa under the control of Scer\GAL430A induces ectopic eye formation on the wing hinge.

Flies expressing eyScer\UAS.cHa under the control of Scer\GAL4lz-gal4 show defects in ommatidial shape and pigment. Rhabdomere morphogenesis and ommatidial rotation are abnormal.

A small cluster of differentiating ectopic ommatidia (occupying approximately 15% of the disc surface area) are formed in the wing disc in animals expressing eyScer\UAS.cHa under the control of Scer\GAL4ap-md544.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 results in ectopic eyes in the wing, antenna and leg. Photoreceptors formation is induced in the third instar larval wing disc.

Expression of eyUAS.cHa under the control of Scer\GAL4ey.PU leads to small and rough eyes when compared to controls.

Overexpression of eyScer\UAS.cHa under the control of Scer\GAL4ey-OK107 results in larval lethality. The larval mushroom bodies of these animals exhibit marked abnormalities, with 73% exhibiting either degeneration or absence of the dorsal lobes.

Expression of eyScer\UAS.cHa via Scer\GAL4cb41, Scer\GAL4cb49 or Scer\GAL4sd.PU fails to induce ectopic eyes.

Flies expressing eyScer\UAS.cHa under the control of Scer\GAL4Bx-MS1096 have ectopic eye structures in the wing.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 in the antennal, wing or leg disc, causes a subset of cells to be transformed into photoreceptors.

Expression of eyScer\UAS.cHa driven by Scer\GAL4Bx-MS1096, induces ectopic eyes on the wing hinge, but not on the head.

eyScer\UAS.cHa, expressed under the control of Scer\GAL4hs.2sev, generates ectopic "eyes" (using Ecol\lacZGMR.T:SV40\nls2 as a marker to visualize eye differentiation) in leg and wing discs, but only when expressed at ~80h after egg laying (AEL), which is the time of endogenous sensory organ development. These ectopic "eyes" are formed only in small groups of cells located close to the anterior-posterior compartment boundary. Ectopic expression of eyScer\UAS.cHa, driven by Scer\GAL4Act5C.PI, produces ectopic eyes in the wing blade and leg tibial segment; these eyes are surrounded by long sensory bristles that resemble the hairs that surround the endogenous eye.

When eyScer\UAS.cHa is driven by Scer\GAL4dpp.blk1 an ectopic eye phenotype is seen.

When eyScer\UAS.cHa is driven by Scer\GAL4dpp.blk1, ectopic eyes are seen. These are seen at the base of antennae, wings and halteres and on leg segments. No extra photoreceptors are detected at the ventral margin in the eye-antennal disc.

When clones expressing eyScer\UAS.cHa (driven by Scer\GAL4Act5C.PI) are made in the eye disc, no effect on growth or photoreceptor differentiation is seen when posterior to the morphogenetic furrow (MF), no affected growth or induced ectopic eyes when anterior to the MF, except that ectopic photoreceptors are induced in 6% of the clones in the ocellar region. In wing discs clones are associated with overgrowths, whereas eye induction is observed in about a third of clones that are located within the posterior compartment or along the anterior-posterior compartmental boundary. the induction of eye is not dependant on the size of the clone. No clones in the Anterior compartment show ectopic eye induction, even if the clone is quite large. When clones expressing eyScer\UAS.cHa (driven by Scer\GAL4bi-omb-Gal4, Scer\GAL430A or Scer\GAL4ap-md544) in the wing disc, ectopic eyes are only seen when clones are made at the A/P border or in the posterior compartment. This is true whether clones are induced in the first, second and early third larval instar stages, though frequency of eye induction is highest when induced in the first instar.

Flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 show a transformation of wing hinge to eye. Wing blade development is partially suppressed.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4ey.PH results in flies with reduced eyes. Most of the flies eclose.

When eyScer\UAS.cHa is driven by Scer\GAL4sca-537.4, strong defects in the formation of the embryonic CNS are seen. In the ventral nerve cord of these embryos, the longitudinal axon pathways are severely disrupted, the anterior and posterior commissures are fused, the segmental and intersegmental nerves are perturbed and the spatial pattern of the majority of the glial cells is deranged. The midline glial cells are positioned correctly. Sever axonal defects are also seen in the developing brain. The tritocerebral commissure is missing in almost all embryos and the major preoral brain commissure is absent approximately half the time. Furthermore, the longitudinal pathways of the embryonic brain, including the embryonic circumesophageal connectives are strongly reduced or absent, and the frontal connectives are absent or misrouted. However no gross perturbations in the overall formation of the neuromeres are seen in the embryonic brain.

Expression of eyScer\UAS.cHa driven by Scer\GAL4dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 results in the induction of ectopic eyes.

When expression is driven by Scer\GAL4238Y, 72% of larvae show unilateral or bilateral reduction or absence of dorsal lobes. A smaller fraction had fusion of medial lobes. The number of Kenyon cells is reduced. The diameter of the MB peduncle is not reduced. When expression is driven by Scer\GAL4hs.PB at early stages of embryogenesis the embryos do not survive. But overexpression at stage 16 causes similar dorsal lobe defects to those seen with Scer\GAL4238Y.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4E132 results in the formation of ectopic eyes. Ectopic eyes are produced at a frequency of 100% if eyScer\UAS.cHa is expressed under the control of Scer\GAL4dpp.blk1.

Expression of eyScer\UAS.cHa under the control of Scer\GAL430A results in the induction of neurons at the anterior/posterior boundary of the wing disc and dorsal to the wing pouch. Ectopic neurons are also induced on the ventral side of the haltere disc. Flies expressing eyScer\UAS.cHa under the control of Scer\GAL430A do not develop ectopic eyes on the haltere and only small patches of retinal tissue appear on the ventral side of the wing hinge at 20% penetrance.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4unspecified produces a disrupted eye phenotype.

When expression is driven by Scer\GAL4dpp.blk1, extra eye structures appear on otherwise deformed leg.

Flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 in a wild-type background develop ectopic eyes on the antennal portion of the eye-antennal discs and on the leg and wing discs. Ommatidial development is also sometimes seen on the haltere and proboscis.

Scer\GAL4dpp.blk1 mediated expression in dac1 or dac3 mutant background is unable to induce ectopic eye formation.

When expressed via Scer\GAL4E132, ectopic eyes are induced in the wing, leg, antenna and haltere. At 25oC 100% of flies produce ectopic eye structures. In some cases the ectopic eyes cause pattern duplications in the surrounding tissues. When expressed via Scer\GAL4MS941, all flies produce eye facets on both wings. When expressed via Scer\GAL4p339, red pigment spots are formed, with 100% penetrance. Ectopic eyes most frequently occur on structures derived from the antennal and wing discs. Distinct ommatidia have lenses and interommatidial bristles. The array of facets and bristles is largely normal. The eye structures on the legs are on average smaller than those on antennae or wings but have relatively normal organization. Ectopic eyes have normal photoreceptor cells (with rhabdomeres), cornea, cone cells and primary, secondary and tertiary pigment cells. The sequence of neuronal differentiation of the normal eye is retained in the ectopic eye cells. Morphogenesis of the ectopic eyes is normal and probably involves the formation of an ectopic morphogenetic furrow.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

Scer\GAL4ey.PU, eyUAS.cHa has eye phenotype, suppressible | partially by CtBP[+]/CtBP87De-10

NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Statement
Reference

AntpUAS.cBa, Scer\GAL4dpp.blk1, eyUAS.cHa has neuron & dorsal mesothoracic disc phenotype

Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of eyScer\UAS.cHa in third instar larval wing discs already expressing dlg1HMS01954 under the control of Scer\GAL4Bx-MS1096 induces ectopic photoreceptor formation in a small proportion of cells, as compared to controls.

Co-expression of sdScer\UAS.cUa with eyScer\UAS.cHa significantly inhibits the ability of eyScer\UAS.cHa to promote cell growth in the notum region. In addition, co-expression of sdScer\UAS.cUa with eyScer\UAS.cHa can also inhibit cell growth in the pouch and hinge regions as shown by decreased clone size.

Co-expression of daScer\UAS.cGa results in increased size of ectopic eyes induced by Scer\GAL430A>eyScer\UAS.cHa.

Co-expression of dada-da.cTa.Scer\UAS results in increased size of ectopic eyes induced by Scer\GAL430A>eyScer\UAS.cHa.

Co-expression of ato::daScer\UAS.cTa results in a moderate decrease in the size of ectopic eyes induced by Scer\GAL430A>eyScer\UAS.cHa.

Co-expression of either dppScer\UAS.cSa, hhScer\UAS.cCa or NICN.Scer\UAS results in enlarged wing discs containing a larger field (30-40% of disc surface area) of differentiating ectopic eye precursors compared to expression of eyScer\UAS.cHa alone under the control of Scer\GAL4ap-md544.

Expression of ctScer\UAS.cPa almost completely suppresses the ectopic eye phenotype seen in adult flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1. The photoreceptor induction seen in the wing discs is also suppressed.

Expression of hthScer\UAS.cPa almost completely suppresses the ectopic eye phenotype seen in adult flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1. The photoreceptor induction seen in the wing discs is also suppressed.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4toy1 partially suppresses the pupal lethality found in toyhdl mutants, increasing the percentage of escapers from 3.75 to 14.1%.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4toy1 or Scer\GAL4toy2 suppresses the ocellar phenotype found in toyhdl mutants in 83% of adult escapers.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4toy1 partially suppresses the pupal lethality found in toyhdl mutants, increasing the percentage of escapers from 5.1 to 9.5%.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4toy1 or Scer\GAL4toy2 suppresses the ocellar phenotype found in toyG7.39 mutants in 85% of adult escapers.

Co-expression of eyScer\UAS.cHa enhances the loss of eye phenotype caused by expression of HScer\UAS.cGa under the control of Scer\GAL4ey.PH.

Co-expression of eyScer\UAS.cHa rescues eye formation in animals expressing AntpScer\UAS.cBa under the control of Scer\GAL4ey-OK107.

Co-expression of Nf-YAScer\UAS.T:Ivir\HA1 partially suppresses the induction of ectopic eye structures in the wing which is caused by expression of eyScer\UAS.cHa under the control of Scer\GAL4Bx-MS1096.

Scer\GAL4ey.PH-mediated expression of eyScer\UAS.cHa can partially rescue the small eye phenotype seen in flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ey.PH in some flies, but most often enhances this phenotype and induces the expression of tiny eyes in different places of the head.

Coexpression of eyScer\UAS.cHa and tkvCA.Scer\UAS under control of Scer\GAL4hs.2sev results in large ectopic "eyes" (using antibodies against elav to visualize eye differentiation) in both wing and leg discs in regions far from the anterior-posterior compartment boundary. Coexpression of wgScer\UAS.cLa with eyScer\UAS.cHa, under control of Scer\GAL4dpp.blk1, completely blocks ectopic eye formation (using antibodies against elav to visualize eye differentiation) in leg and wing discs.

Co-expression of eyScer\UAS.cHa with pbScer\UAS.A (driven by Scer\GAL4dpp.blk1) leads to a suppression of the ectopic eye phenotype seen in eyScer\UAS.cHa,Scer\GAL4dpp.blk1 animals.

When eyScer\UAS.cHa and eygScer\UAS.lune are driven by Scer\GAL4dpp.blk1, the ectopic eyes produced are larger than eyScer\UAS.cHa or eygScer\UAS.lune driven alone. This phenotype is more noticeable in the eye disc. The addition of eyScer\UAS.cHa driven by Scer\GAL4ey.PH can partially rescue the eygM3-12/eyg1 mutant eye phenotype.

When hhScer\UAS.cAa and eyScer\UAS.cHa are expressed in clones (under the control of Scer\GAL4Act5C.PI) in the anterior compartment of the wing disc, 46% of clones cause ectopic eye formation. When hhScer\UAS.cAa and eyScer\UAS.cHa are expressed under the control of Scer\GAL430A in the anterior compartment of the wing disc, ectopic eye formation is seen at 100% penetrance, although the size of these ectopic eyes varies from disc to disc. When eyScer\UAS.cHa clones (driven by Scer\GAL4Act5C.PI) are made in the wing disc in a hhbar3 background no ectopic eyes are seen. When ciCe-2.Scer\UAS.T:Ivir\HA1 and eyScer\UAS.cHa co-expressed (driven by Scer\GAL4Act5C.PI) in clones in the wing disc, morphogenetic furrow initiation and photoreceptor differentiation are blocked within the clones. When ciScer\UAS.T:Ivir\HA1 and eyScer\UAS.cHa are co-expressed in the wing disc (driven by Scer\GAL4bi-omb-Gal4) ectopic eyes are induced in both anterior and posterior compartments. These eyes are larger than those seen in eyScer\UAS.cHa, eyScer\UAS.cHa animals alone. When dppScer\UAS.cSa, hhScer\UAS.cAa and eyScer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dppScer\UAS.cSa, hhScer\UAS.cAa and eyScer\UAS.cHa driven by Scer\GAL4bi-omb-Gal4, causes a high larval lethality at 25oC. The addition of brkScer\UAS.cJa suppresses the ectopic eye phenotype seen in eyScer\UAS.cHa, Scer\GAL4bi-omb-Gal4 animals. The addition of brkScer\UAS.cJa suppresses the ectopic eye phenotype seen in eyScer\UAS.cHa, Scer\GAL4bi-omb-Gal4 animals. The addition of brkScer\UAS.cJa suppresses the ectopic eye phenotype seen in eyScer\UAS.cHa, Scer\GAL4dpp.blk1 animals.

When dppScer\UAS.cSa and eyScer\UAS.cHa are driven by Scer\GAL430A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment. When dppScer\UAS.cSa, eyScer\UAS.cHa and hhScer\UAS.cCa are driven by Scer\GAL430A, photoreceptor differentiation is induced in both compartments of the wing disc. When dppScer\UAS.cSa, eyaScer\UAS.cPa and eyScer\UAS.cHa are driven by Scer\GAL430A, photoreceptor differentiation is induced in both compartments of the wing disc. The addition of soScer\UAS.cPa increases the penetrance of this phenotype. When hhScer\UAS.cCa, eyaScer\UAS.cPa and eyScer\UAS.cHa are driven by Scer\GAL430A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment. The addition of dppScer\UAS.cSa and eyaScer\UAS.cPa (driven by Scer\GAL4ey.PB) to animals with smoD16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued. If soScer\UAS.cPa is also added, posterior margin clones are still rescued, although ectopic anterior furrows are induced with high frequency.

The headless phenotype caused by expression of gsbScer\UAS.cJa under the control of Scer\GAL4ey.PH is partially rescued by coexpression of eyScer\UAS.cHa, resulting in small-eyed flies.

Co-expression of eyScer\UAS.cHa and AntpScer\UAS.cBa when driven by Scer\GAL4dpp.blk1 leads to a inhibition of neuronal differentiation in the wing disc. Co-expression of eyScer\UAS.cHa and AntpScer\UAS.cBa when driven by Scer\GAL4ey.PH repsresses the AntpScer\UAS.cBa eye phenotypes.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4ey.PH interferes with dppd-blk eye development to a variable extent, often resulting in smaller eyes.

Flies expressing NECN.Scer\UAS.cGa and eyScer\UAS.cHa driven by Scer\GAL4dpp.blk1 exhibit ectopic eyes on legs and wings in all flies.

When eyScer\UAS.cHa and dacScer\UAS.cSa are coexpressed under the control of Scer\GAL430A, large ectopic eyes are seen on the wing and haltere hinges with complete penetrance. Clear ommatidial structures are seen on both the dorsal and ventral side of the wing hinge and the structure of the lens and interommatidial bristles is similar to wild-type.

Expression of eyScer\UAS.cHa using Scer\GAL4dpp.blk1 in a so1/so1 background does not lead to the formation of ectopic eyes.

When expression is driven by Scer\GAL4dpp.blk1 in a so1 or eya1 background, no eye structures appear but legs are reduced/deformed. When expression is driven by Scer\GAL4dpp.blk1 in a so1 background ectopic cell death occurs in the region of the wing disc that would lead to extra eyes in a wild type background.

Flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 in a eya2 background do not develop ectopic eyes. Ectopic eye formation in flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1 is greatly enhanced if the flies also carry eyaScer\UAS.cBa; the ectopic eyes formed in flies expressing both eyScer\UAS.cHa and eyaScer\UAS.cBa are larger and form with higher penetrance than in flies expressing eyScer\UAS.cHa alone, and eye formation occurs in the genitalia in flies expressing both eyScer\UAS.cHa and eyaScer\UAS.cBa.

Xenogenetic Interactions
Statement
Reference

The disrupted eye phenotype produced by expression of eyScer\UAS.cHa under the control of Scer\GAL4unspecified is not modified by coexpression of Hsap\HSPA1LScer\UAS.cWa.

Complementation and Rescue Data
Comments

Expression of eyScer\UAS.cHa under the control of Scer\GAL4ey-OK107 rescues the eye, mushroom body and central complex defects of eyEH/eyOK107-16 and eyJD/eyOK107-16 mutants.

Expression of eyScer\UAS.cHa under the control of Scer\GAL4ey.PH significantly rescues the lethality seen in eyD homozygous mutants. The flies that eclose from pupae exhibit smaller than normal heads and a majority of the compound eyes are reduced in size, while the ocelli are normal. The viability of these flies is more efficient at 18[o]C compared to 25[o]C and 29[o]C. The rescue efficiencies of flies carrying both driver and effector constructs or effector construct alone (i.e only eyScer\UAS.cHa) are similar.

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Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
eyScer\UAS.cHa
eyUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Halder
Secondary FlyBase IDs
    References (63)