FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\eyJD
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General Information
Symbol
Dmel\eyJD
Species
D. melanogaster
Name
FlyBase ID
FBal0117526
Feature type
allele
Associated gene
Dmel\ey
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

antimorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Benassayag et al., 2003)
Mutagen
ethyl methanesulfonate
(Benassayag et al., 2003, Callaerts et al., 2001)
Progenitor genotype
Cytology
Description

eyJD encodes a peptide truncated at amino acid 432.

(Benassayag et al., 2003)

Amino acid replacement: R433term.

(Callaerts et al., 2001)

Nucleotide substitution: C1382T.

(Callaerts et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
4:717,662..717,662 [+]
Nucleotide change:

C717662T

Reported nucleotide change:

C1382T

Amino acid change:

R432term | ey-PA; R218term | ey-PB; R451term | ey-PC; R492term | ey-PD

Reported amino acid change:

R433term

Comment:

@C_to_T_transition ; SO:1000011

Comment:

@nonsense_codon_change_in_transcript ; SO:1000062

(Callaerts et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult brain

      (Callaerts et al., 2001)

      adult central complex

      (Callaerts et al., 2001)

      adult central complex (with Df(4)J2)

      (Callaerts et al., 2001)

      adult central complex (with eyJ5.71)

      (Callaerts et al., 2001)

      adult central complex (with eyOK107-4)

      (Clements et al., 2009)

      adult central complex (with eyOK107-6)

      (Clements et al., 2009)

      adult central complex (with eyOK107-16)

      (Clements et al., 2009)

      adult median bundle (with eyD1Da)

      (Clements et al., 2008)

      adult mushroom body

      (Callaerts et al., 2001, Martini et al., 2000)

      adult mushroom body (with Df(4)J2)

      (Callaerts et al., 2001)

      adult mushroom body (with eyJ5.71)

      (Callaerts et al., 2001)

      adult mushroom body (with eyOK107-4)

      (Clements et al., 2009)

      adult mushroom body (with eyOK107-6)

      (Clements et al., 2009)

      adult mushroom body (with eyOK107-10)

      (Clements et al., 2009)

      adult mushroom body (with eyOK107-16)

      (Clements et al., 2009)

      adult optic lobe

      (Callaerts et al., 2001)

      adult pars intercerebralis (with eyD1Da)

      (Clements et al., 2008)

      antenna

      (Benassayag et al., 2003)

      arista

      (Benassayag et al., 2003)

      calyx of adult mushroom body

      (Callaerts et al., 2001)

      ellipsoid body

      (Callaerts et al., 2001)

      embryonic/larval hemolymph | late third instar larval stage

      (Clements et al., 2008)

      eye

      (Benassayag et al., 2003, Callaerts et al., 2001)

      eye (with Df(4)BA)

      (Benassayag et al., 2003)

      eye (with Df(4)J2)

      (Callaerts et al., 2001)

      eye (with eyJ5.71)

      (Callaerts et al., 2001)

      eye (with eyOK107-4)

      (Clements et al., 2009)

      eye (with eyOK107-6)

      (Clements et al., 2009)

      eye (with eyOK107-10)

      (Clements et al., 2009)

      eye (with eyOK107-16)

      (Clements et al., 2009)

      eye (with eyR)

      (Callaerts et al., 2001)

      fan-shaped body

      (Callaerts et al., 2001)

      lobe system of adult mushroom body

      (Callaerts et al., 2001)

      maxillary palp

      (Benassayag et al., 2003)

      mushroom body & neuron

      (Martini et al., 2000)

      neurite (with eyD1Da)

      (Clements et al., 2008)

      third segment of antenna

      (Benassayag et al., 2003)

      wing (with eyD1Da)

      (Clements et al., 2008)
      Detailed Description
      Statement
      Reference

      eyD1Da/eyJD mutant flies are smaller in size than wild-type. Wing size is reduced by approximately 10%, and the average weight of mutant flies is between 66-68% of wild-type body weight.

      A 10% increase in larval hemolymph glucose and trehalose levels is observed in eyJD homozygous mutant third instar larvae compared to control hemolymph.

      In eyD1Da/eyJD mutant brains, the morphology of the insulin producing cells (IPCs) is abnormal. Clustering of the IPC somata is disrupted in mutants, with disorganisation of the IPC cluster and reduced cell size. Overgrowth of IPC neurites along the dorsal-most periphery of the brain is frequently observed. The IPC axonal projections exhibit morphological defects. Proximal to the somata, the median bundle is thinner than in controls. Further distal, the median bundle displays a prominent defasciculation phenotype in which axons defasciculate from the median bundle as they project ventrally.

      (Clements et al., 2008)

      Heterozygotes show no marked defects. Homozygous adults show a complete eye loss phenotype and the fusion of antenna and maxillary palp, but normal labial palps. The antennal segment A3 (ant) may be malformed, slightly enlarged and extended from the head. The arista can be shortened, curved and thickened at the base, malformed and extended from the head. Homozygotes animals show a stronger phenotype than eyJD/Df(4)BA animals.

      (Benassayag et al., 2003)

      Homozygotes show 85% lethality. eyJD/Df(4)J2 flies show 94% lethality. Eyes are reduced in size in homozygotes; 75% have eyes that are 0-25% of wild-type size and 25% have eyes that are 25-50% of wild-type size. Homozygous adults have smaller brains than normal, with defects in the optic lobes, the central complex and the mushroom bodies. The mushroom body lobes are generally not recognisable in homozygous flies. The calyces of the mushroom bodies are reduced in size. In the central complex, the ellipsoid body appears to be fused to the fan-shaped body and both these neuropils are almost fused with the surrounding protocerebral neuropil. The total volume of the central complex is almost unchanged compared to wild type. Abnormal neuronal projections are seen in the ellipsoid and fan-shaped bodies. Homozygous flies are extremely reluctant to walk, both in "Buridan's paradigm" and a stepping analyser experiment. Eyes are reduced in size in eyJD/Df(4)J2 flies; 33% have eyes that are 0-25% of wild-type size, 50% have eyes that are 25-50% of wild-type size, 17% have eyes that are 50-75% of wild-type size and 0% have eyes that are 75-100% of wild-type size. eyJD/Df(4)J2 flies show severe mushroom body and central complex defects. 100% of ey2/eyJD flies have eyes which are 75-100% of wild-type size and they have no overt defects in the mushroom body or central complex. 97% of eyR/eyJD flies have eyes which are 75-100% of wild-type size and 3% have eyes that are 50-75% of wild-type size. The flies have no overt defects in the mushroom body or central complex. eyJD/eyJ5.71 flies show 72% lethality. Eyes are reduced in size in eyJD/eyJ5.71 flies; 22% have eyes that are 0-25% of wild-type size, 72% have eyes that are 25-50% of wild-type size, 6% have eyes that are 50-75% of wild-type size and 0% have eyes that are 75-100% of wild-type size. eyJD/eyJ5.71 flies show severe mushroom body and central complex defects.

      (Callaerts et al., 2001)

      The number of mushroom body neurons and the size of the mushroom body neuropil is reduced in homozygous adults.

      (Martini et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      ey[+]/eyJD is an enhancer of visible phenotype of pbRev3.HSPB

      (Callaerts et al., 2001)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      eyJD is an enhancer of eye phenotype of pbRev3.HSPB

      (Benassayag et al., 2003)

      ey[+]/eyJD is an enhancer of eye phenotype of pbRev3.HSPB

      (Callaerts et al., 2001)
      NOT Enhancer of
      Statement
      Reference

      ey[+]/eyJD is a non-enhancer of phenotype of dacP

      (Martini et al., 2000)

      ey[+]/eyJD is a non-enhancer of mushroom body phenotype of dacP

      (Martini et al., 2000)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The combination of 2 copies of eyJD and pbRev3.HSPB often leds to flies with complete eye loss and the fusion of the antenna and maxillary palp. In some cases, the maxillary palp, remains adjoined to the antennal appendage. However differentiation od the proboscis is not affected.

      (Benassayag et al., 2003)

      eyJD dominantly enhances the eye loss phenotype of pbRev3.HSPB flies.

      (Callaerts et al., 2001)

      dac1; eyJD, dac3; eyJD and Df(2L)dac-4; eyJD double heterozygotes have normal mushroom bodies and eyes.

      (Martini et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Allelic series based on mutant phenotype; ey11 > eyD1Da > eyEH > eyJD.

      (Benassayag et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      eyJD
      (Clements et al., 2009, Clements et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)