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General Information
Symbol
Dmel\eyD1Da
Species
D. melanogaster
Name
FlyBase ID
FBal0117527
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A region of 37bp is deleted and replaced by 5bp (GTCGG). This results in a frameshift and premature translation termination. A protein of 682 amino acids is produced, the last 29 of which are unrelated to wild type ey sequence.

Inserted_sequence:

GTCGG

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

eyD1Da encodes a peptide truncated at amino acid 682.

37bp deletion (from position 2051 to 2080) is replaced by 5bp (GTCGG) in exon 9. This results in a frameshift and premature translation termination at position 2170. The predicted protein produced is 682 amino acids with the last 29 being unrelated to wild-type ey sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

mushroom body & neuron

Detailed Description
Statement
Reference

eyD1Da/eyJD mutant flies are smaller in size than wild-type. Wing size is reduced by approximately 10%, and the average weight of mutant flies is between 66-68% of wild-type body weight.

In eyD1Da/eyJD mutant brains, the morphology of the insulin producing cells (IPCs) is abnormal. Clustering of the IPC somata is disrupted in mutants, with disorganisation of the IPC cluster and reduced cell size. Overgrowth of IPC neurites along the dorsal-most periphery of the brain is frequently observed. The IPC axonal projections exhibit morphological defects. Proximal to the somata, the median bundle is thinner than in controls. Further distal, the median bundle displays a prominent defasciculation phenotype in which axons defasciculate from the median bundle as they project ventrally.

Homozygotes have reduced eyes. The ellipsoid body of the adult brain is disrupted.

Homozygotes show 13% lethality. eyD1Da/Df(4)J2 flies show 29% lethality.

Eyes are reduced in size in homozygotes; 14% have eyes that are 0-25%

of wild-type size, 14% have eyes that are 25-50% of wild-type size,

72% have eyes that are 50-75% of wild-type size and 0% have eyes that

are 75-100% of wild-type size.

Homozygous adults have smaller brains than normal, with defects in

the optic lobes, the central complex and the mushroom bodies. In the

optic lobe, the lamina is often smaller and flatter than normal, the

medulla is reduced in size and mispositioned, the medulla cortex is

severely underdeveloped and the serpentine layer (which separates the

distal and proximal part of the medulla) is disorganised. The lobula

and lobula plate are reduced in size, the internal chiasm is abnormal

and ectopic fibre bundles which appear to originate directly from the

lamina are seen in the lobula/lobula plate complex. Fusion of the

optic lobe with the central brain is occasionally seen. The mushroom

body lobes are generally not recognisable in homozygous flies, while

peduncles are seen in most brains, but they are greatly reduced in

diameter. The calyces of the mushroom bodies are reduced in size.

In the central complex, the ellipsoid body appears to be fused to

the fan-shaped body and both these neuropils are not well separated

from the surrounding protocerebral neuropil. The protocerebral bridge

is disintegrated into several chunks of neuropil. The total volume

of the central complex is almost unchanged compared to wild type.

Abnormal neuronal projections are seen in the ellipsoid and fan-shaped

bodies.

Homozygous flies are extremely reluctant to walk, both in "Buridan's

paradigm" and a stepping analyser experiment.

Eyes are reduced in size in eyD1Da/Df(4)J2 flies; 31% have eyes

that are 0-25% of wild-type size, 25% have eyes that are 25-50% of

wild-type size, 43% have eyes that are 50-75% of wild-type size and

1% have eyes that are 75-100% of wild-type size.

eyD1Da/Df(4)J2 flies show severe mushroom body and central complex

defects.

100% of ey2/eyD1Da flies have eyes which are 75-100% of wild-type

size and they have no overt defects in the mushroom body or central

complex.

89% of eyR/eyD1Da flies have eyes which are 75-100% of wild-type

size, 9% have eyes that are 50-75% of wild-type size, 1% have eyes

that are 25-50% of wild-type size and 1% have eyes that are 0-25% of

wild-type size. The flies have no overt defects in the mushroom body

or central complex.

Eyes are reduced in size in eyD1Da/eyJ5.71 flies; 43% have

eyes that are 0-25% of wild-type size, 14% have eyes that are 25-50%

of wild-type size, 38% have eyes that are 50-75% of wild-type size

and 5% have eyes that are 75-100% of wild-type size.

eyD1Da/eyJ5.71 flies show severe mushroom body and central

complex defects.

The number of mushroom body neurons and the size of the mushroom body neuropil is reduced in homozygous adults.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

ey[+]/eyD1Da is an enhancer of visible phenotype of pbRev3.HSPB

Phenotype Manifest In
Enhancer of
Statement
Reference

eyD1Da is an enhancer of eye phenotype of pbRev3.HSPB

ey[+]/eyD1Da is an enhancer of eye phenotype of pbRev3.HSPB

NOT Enhancer of
Statement
Reference

ey[+]/eyD1Da is a non-enhancer of phenotype of dacP

ey[+]/eyD1Da is a non-enhancer of mushroom body phenotype of dacP

Additional Comments
Genetic Interactions
Statement
Reference

eyD1Da dominantly enhances the eye loss phenotype of pbRev3.HSPB flies.

dac1; eyD1Da, dac3; eyD1Da and Df(2L)dac-4; eyD1Da double heterozygotes have normal mushroom bodies and eyes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Allelic series based on mutant phenotype; ey11 > eyD1Da > eyEH > eyJD.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)