FlyBase Allele Report: Dmel\ey[D1Da]

General Information

Symbol

Dmel\ey^D1Da

Species

D. melanogaster

Name

FlyBase ID

FBal0117527

Feature type

allele

Associated gene

Dmel\ey

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

diepoxybutane

Nature of the Allele

Allele class

Mutagen

diepoxybutane

(Benassayag et al., 2003, Callaerts et al., 2001)

Progenitor genotype

Cytology

Description

ey^D1Da encodes a peptide truncated at amino acid 682.

(Benassayag et al., 2003)

37bp deletion (from position 2051 to 2080) is replaced by 5bp (GTCGG) in exon 9. This results in a frameshift and premature translation termination at position 2170. The predicted protein produced is 682 amino acids with the last 29 being unrelated to wild-type ey sequence.

(Callaerts et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

4:720,369..720,405 [+]

Comment:

A region of 37bp is deleted and replaced by 5bp (GTCGG). This results in a frameshift and premature translation termination. A protein of 682 amino acids is produced, the last 29 of which are unrelated to wild type ey sequence.

Inserted_sequence:

GTCGG

(Callaerts et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior

(Callaerts et al., 2001)

abnormal neuroanatomy

(Callaerts et al., 2001)

abnormal neuroanatomy (with Df(4)J2)

(Callaerts et al., 2001)

abnormal neuroanatomy (with ey^J5.71)

(Callaerts et al., 2001)

decreased body size (with ey^JD)

(Clements et al., 2008)

decreased cell size (with ey^JD)

(Clements et al., 2008)

partially lethal (with Df(4)J2)

(Callaerts et al., 2001)

partially lethal | recessive

(Callaerts et al., 2001)

viable (with ey²)

(Callaerts et al., 2001)

viable (with ey^J5.71)

(Callaerts et al., 2001)

viable (with ey^R)

(Callaerts et al., 2001)

visible

(Niimi et al., 2002, Callaerts et al., 2001)

visible (with Df(4)J2)

(Callaerts et al., 2001)

visible (with ey^J5.71)

(Callaerts et al., 2001)

visible (with ey^OK107-4)

(Clements et al., 2009)

visible (with ey^OK107-6)

(Clements et al., 2009)

visible (with ey^OK107-10)

(Clements et al., 2009)

visible (with ey^OK107-16)

(Clements et al., 2009)

visible (with ey^R)

(Callaerts et al., 2001)

Phenotype Manifest In

adult brain

(Callaerts et al., 2001)

adult central complex

(Callaerts et al., 2001)

adult central complex (with Df(4)J2)

(Callaerts et al., 2001)

adult central complex (with ey^J5.71)

(Callaerts et al., 2001)

adult central complex (with ey^OK107-4)

(Clements et al., 2009)

adult central complex (with ey^OK107-6)

(Clements et al., 2009)

adult central complex (with ey^OK107-10)

(Clements et al., 2009)

adult central complex (with ey^OK107-16)

(Clements et al., 2009)

adult median bundle (with ey^JD)

(Clements et al., 2008)

adult mushroom body

(Callaerts et al., 2001, Martini et al., 2000)

adult mushroom body (with Df(4)J2)

(Callaerts et al., 2001)

adult mushroom body (with ey^J5.71)

(Callaerts et al., 2001)

adult mushroom body (with ey^OK107-4)

(Clements et al., 2009)

adult mushroom body (with ey^OK107-6)

(Clements et al., 2009)

adult mushroom body (with ey^OK107-10)

(Clements et al., 2009)

adult mushroom body (with ey^OK107-16)

(Clements et al., 2009)

adult optic lobe

(Callaerts et al., 2001)

adult pars intercerebralis (with ey^JD)

(Clements et al., 2008)

calyx of adult mushroom body

(Callaerts et al., 2001)

cell body rind of adult medulla

(Callaerts et al., 2001)

ellipsoid body

(Niimi et al., 2002, Callaerts et al., 2001)

eye

(Niimi et al., 2002, Callaerts et al., 2001)

eye (with Df(4)J2)

(Callaerts et al., 2001)

eye (with ey^J5.71)

(Callaerts et al., 2001)

eye (with ey^OK107-4)

(Clements et al., 2009)

eye (with ey^OK107-6)

(Clements et al., 2009)

eye (with ey^OK107-10)

(Clements et al., 2009)

eye (with ey^OK107-16)

(Clements et al., 2009)

eye (with ey^R)

(Callaerts et al., 2001)

fan-shaped body

(Callaerts et al., 2001)

lamina

(Callaerts et al., 2001)

lobe system of adult mushroom body

(Callaerts et al., 2001)

lobula

(Callaerts et al., 2001)

lobula plate

(Callaerts et al., 2001)

medulla

(Callaerts et al., 2001)

medulla serpentine layer

(Callaerts et al., 2001)

mushroom body & neuron

(Martini et al., 2000)

neurite (with ey^JD)

(Clements et al., 2008)

protocerebral bridge

(Callaerts et al., 2001)

wing (with ey^JD)

(Clements et al., 2008)

Detailed Description

Statement

Reference

ey^D1Da/ey^JD mutant flies are smaller in size than wild-type. Wing size is reduced by approximately 10%, and the average weight of mutant flies is between 66-68% of wild-type body weight.

In ey^D1Da/ey^JD mutant brains, the morphology of the insulin producing cells (IPCs) is abnormal. Clustering of the IPC somata is disrupted in mutants, with disorganisation of the IPC cluster and reduced cell size. Overgrowth of IPC neurites along the dorsal-most periphery of the brain is frequently observed. The IPC axonal projections exhibit morphological defects. Proximal to the somata, the median bundle is thinner than in controls. Further distal, the median bundle displays a prominent defasciculation phenotype in which axons defasciculate from the median bundle as they project ventrally.

(Clements et al., 2008)

Homozygotes have reduced eyes. The ellipsoid body of the adult brain is disrupted.

(Niimi et al., 2002)

Homozygotes show 13% lethality. ey^D1Da/Df(4)J2 flies show 29% lethality.

Eyes are reduced in size in homozygotes; 14% have eyes that are 0-25%

of wild-type size, 14% have eyes that are 25-50% of wild-type size,

72% have eyes that are 50-75% of wild-type size and 0% have eyes that

are 75-100% of wild-type size.

Homozygous adults have smaller brains than normal, with defects in

the optic lobes, the central complex and the mushroom bodies. In the

optic lobe, the lamina is often smaller and flatter than normal, the

medulla is reduced in size and mispositioned, the medulla cortex is

severely underdeveloped and the serpentine layer (which separates the

distal and proximal part of the medulla) is disorganised. The lobula

and lobula plate are reduced in size, the internal chiasm is abnormal

and ectopic fibre bundles which appear to originate directly from the

lamina are seen in the lobula/lobula plate complex. Fusion of the

optic lobe with the central brain is occasionally seen. The mushroom

body lobes are generally not recognisable in homozygous flies, while

peduncles are seen in most brains, but they are greatly reduced in

diameter. The calyces of the mushroom bodies are reduced in size.

In the central complex, the ellipsoid body appears to be fused to

the fan-shaped body and both these neuropils are not well separated

from the surrounding protocerebral neuropil. The protocerebral bridge

is disintegrated into several chunks of neuropil. The total volume

of the central complex is almost unchanged compared to wild type.

Abnormal neuronal projections are seen in the ellipsoid and fan-shaped

bodies.

Homozygous flies are extremely reluctant to walk, both in "Buridan's

paradigm" and a stepping analyser experiment.

Eyes are reduced in size in ey^D1Da/Df(4)J2 flies; 31% have eyes

that are 0-25% of wild-type size, 25% have eyes that are 25-50% of

wild-type size, 43% have eyes that are 50-75% of wild-type size and

1% have eyes that are 75-100% of wild-type size.

ey^D1Da/Df(4)J2 flies show severe mushroom body and central complex

defects.

100% of ey²/ey^D1Da flies have eyes which are 75-100% of wild-type

size and they have no overt defects in the mushroom body or central

complex.

89% of ey^R/ey^D1Da flies have eyes which are 75-100% of wild-type

size, 9% have eyes that are 50-75% of wild-type size, 1% have eyes

that are 25-50% of wild-type size and 1% have eyes that are 0-25% of

wild-type size. The flies have no overt defects in the mushroom body

or central complex.

Eyes are reduced in size in ey^D1Da/ey^J5.71 flies; 43% have

eyes that are 0-25% of wild-type size, 14% have eyes that are 25-50%

of wild-type size, 38% have eyes that are 50-75% of wild-type size

and 5% have eyes that are 75-100% of wild-type size.

ey^D1Da/ey^J5.71 flies show severe mushroom body and central

complex defects.

(Callaerts et al., 2001)

The number of mushroom body neurons and the size of the mushroom body neuropil is reduced in homozygous adults.

(Martini et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ey[+]/ey^D1Da is an enhancer of visible phenotype of pb^Rev3.HSPB

(Callaerts et al., 2001)

Phenotype Manifest In

Enhancer of

Statement

Reference

ey^D1Da is an enhancer of eye phenotype of pb^Rev3.HSPB

(Benassayag et al., 2003)

ey[+]/ey^D1Da is an enhancer of eye phenotype of pb^Rev3.HSPB

(Callaerts et al., 2001)

NOT Enhancer of

Statement

Reference

ey[+]/ey^D1Da is a non-enhancer of phenotype of dac^P

(Martini et al., 2000)

ey[+]/ey^D1Da is a non-enhancer of mushroom body phenotype of dac^P

(Martini et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

ey^D1Da dominantly enhances the eye loss phenotype of pb^Rev3.HSPB flies.

(Callaerts et al., 2001)

dac¹; ey^D1Da, dac³; ey^D1Da and Df(2L)dac-4; ey^D1Da double heterozygotes have normal mushroom bodies and eyes.

(Martini et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ey^OK107-16

(Clements et al., 2009)

ey^OK107-10

(Clements et al., 2009)

ey^OK107-6

(Clements et al., 2009)

ey^OK107-4

(Clements et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series based on mutant phenotype; ey¹¹ > ey^D1Da > ey^EH > ey^JD.

(Benassayag et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ey^D1

(Benassayag et al., 2003)

ey^D1Da

(McDaniel et al., 2025, Clements et al., 2009, Clements et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (7)

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