Open Close
General Information
Symbol
Dmel\ctUAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Park
FlyBase ID
FBal0137283
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-cut, UAS-ct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the 3.4kb ct rb10 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Females expressing ctUAS.cPa under the control of Scer\GAL4GMR44E10 lay much fewer eggs and retain significantly more mature follicles in the ovary, as compared to controls. Consistently, follicles rupture much less frequently upon induction by OA or ionomycin, as compared to controls.

Midgut clones expressing ctUAS.cPa under the control of Scer\GAL4esg-NP5130 mainly contain renal progenitor/principal-like cells (i.e. Pdm1-negative, Cut-positive, polyploid cells), as compared to controls.

Expression of ctScer\UAS.cPa under the control of Scer\GAL4GMR.PU at 19[o]C produces flies that have pale/mottled and glazed eyes (lacking clear separation of ommatidia).

Expression of ctScer\UAS.cPa under the control of Scer\GAL4Bx-MS1096 results in a small-wing phenotype.

Scer\GAL4221 or Scer\GAL498b-mediated expression of ctScer\UAS.cPa induces dendrite overgrowth in each of the C1da neurons (vpda, ddaD, and ddaE). Scer\GAL4221-mediated expression results in 4.8 mm dendrite growth, and Scer\GAL498b results in 3.0 mm dendrite growth.

Expression of ctScer\UAS.cPa under the control of Scer\GAL4221 results in an increase in dendrite complexity and in the formation of spiked protrusions in class I vpda neurons.

Expression of ctScer\UAS.cPa under the control of Scer\GAL4salm-459.2 induces an ectopic actin cable at the interface of the ct expressing and ct non-expressing cells.

Expression of ctScer\UAS.cPa in the mds neurons under the control of Scer\GAL4clh201 increases dendritic branching and causes crawling defets.

Ectopic expression of ctScer\UAS.cPa under the control of Scer\GAL4221 significantly increases the number of dendrite termini by 291%, total arbor length by 106%, and arbor area by 35% over wild-type levels.

Expression of ctScer\UAS.cPa under the control of Scer\GAL4ppk.PG in class IV neurons results in the formation of only a small number of filopodia/spikes.

In ctScer\UAS.cPa; Scer\GAL4GH146 animals there is a medial shift in targeting of both antero-dorsal (adPN) and lateral (lPN) projection neuron dendrites within the antennal lobe. adPN dendrites in these animals avoid antennal glomeruli DM1 and DM2. DL1 adPN have an abnormally diffuse dendritic arbour which targets the medial part of DL1 and adjacent glomeruli.

Expression of ctScer\UAS.cPa under the control of Scer\GAL4C96 disrupts wing margin bristle development.

Mis-expression of ctScer\UAS.cPa, in clones under the regulation of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C during oogenesis does not direct follicle cells into either the polar-cell fate or the stalk-cell fate; instead, they appear to maintain an immature main-body follicle-cell fate. Continuous expression of ctScer\UAS.cPa under the regulation of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C during mid-oogenesis causes the follicle cells to be more densely distributed and their nuclei to be slightly smaller than those of the wild-type.

Expression of ctScer\UAS.cPa in vpda neurons, under the control of Scer\GAL4221, increases the dendritic growth and branching of these neurons.

Expression of ctScer\UAS.cPa in ddaE neurons, under the control of Scer\GAL4109(2)80 and Scer\GAL4IG1-2, induces dendritic overgrowth and overbranching.

When ctScer\UAS.cPa is driven by Scer\GAL4221 a completely penetrant acquisition of exuberant short dendritic branches is seen in the vpda neuron. Ectopic branching is apparent by the first instar stage and persists throughout larval development. These terminal branches are highly dynamic. When ctScer\UAS.cPa is driven by Scer\GAL4109(2)80 an increase in dendritic branches is seen in ddaB neurons. When ctScer\UAS.cPa is driven by Scer\GAL4477 an decrease in dendritic branches is seen in ddaC neurons, significantly shorter terminals with both spiked and curved morphologies are seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of ctScer\UAS.cPa under the control of Scer\GAL4221 in a sn36a background in class I vpda neurons results in an expansion of the dendrite tree. In addition, the formation of ectopic spiked protrusions is reduced by more than 40% compared to the expression of ctScer\UAS.cPa under the control of Scer\GAL4221 in a wild-type background.

Expression of ctScer\UAS.cPa almost completely suppresses the ectopic eye phenotype seen in adult flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1. The photoreceptor induction seen in the wing discs is also suppressed.

Ectopic expression of Rac1Scer\UAS.cLa along with ctScer\UAS.cPa in class I neurons under the control of Scer\GAL4221 leads to the formation of an arbor with many filpodia/spikes, which shows a significant enhancement of branching as compared to either Rac1Scer\UAS.cLa or ctScer\UAS.cPa expression alone.

Co-expression of knScer\UAS.cMa and ctScer\UAS.cPa under the control of Scer\GAL4221 increases the number of dendritic branching points to 409% of wild-type. Similarly, the total length is increased by 130% and the arbor area by 89%.

Expression of ctScer\UAS.cPa under the control of Scer\GAL4ppk.PG in class IV neurons in a kn1/knKN2 results in the formation of many additional filpodia/spikes that closely resemble those of class III neurons. The overall level of branching in class IV neuron dendritic arbors appears similar to those of class III neurons.

In acj66 homozygous somatic clones in animals carrying ctScer\UAS.cPa; Scer\GAL4GH146, DL1 adPN have diffuse dendrites which fail to innervate antennal lobe glomerulus DL1 and instead occupy the medial to dorso-medial antennal lobe: typically VM2, DM6 and DC1.

Coexpression of ctScer\UAS.cPa and abScer\UAS.cBa, under the control of Scer\GAL4221, does not suppress the increased dendritic branching of vpda neurons, seen when ctScer\UAS.cPa is expressed, but did partially rescue the total dendritic length phenotype.

Coexpression of ctScer\UAS.cPa with abScer\UAS.S.cSa, under the control of Scer\GAL4109(2)80 and Scer\GAL4IG1-2, partially suppresses the reduction of field size and terminal number of ddA neurons seen when abScer\UAS.L.cSa is expressed alone. This coexpression also leads to a partial suppression of the dendritic overgrowth and overbranching of ddaE neurons, seen when ctScer\UAS.cPa is expressed under the control of Scer\GAL4109(2)80 and Scer\GAL4IG1-2.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of ctJF03304 partially rescues the wing phenotypes seen when ctScer\UAS.cPa is expressed under the control of Scer\GAL4Bx-MS1096, although the posterior region remains mispatterned.

ctScer\UAS.cPa; Scer\GAL4GH146 completely rescues the Targeting of DMA, DM2 and VA5 antennal glomeruli by lateral projection neurons (lPN) in ctdb3 or ctC145 homozygous clones, but fails to rescue the decrease in vPN cell number and only partially rescues ventral projection neuron (vPN) Targeting of VA1lm but not of DA1.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

M. Park R. Bodmer

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ctScer\UAS.cPa
ctUAS.cPa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Park
Secondary FlyBase IDs
    References (23)