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General Information
Symbol
Dmel\ctC145
Species
D. melanogaster
Name
FlyBase ID
FBal0001952
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
cutC145, ct145, cut145
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Mutation in the coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

joint & antenna | somatic clone

Detailed Description
Statement
Reference

ctC145 mutant clones at the ureter region contain both ectopic Pros-positive enteroendocrine-like cells and ectopic Pdm1-positive enterocyte-like cells, as compared to controls.

An increased frequency of ctC145 mutant wing disc clones (induced during either the second or third instar larval stages) respect the interface between boundary and non-boundary cell compared to wild type.

Cell polarity is not affected in ctC145 mutant embryos.

Homozygous clones in the antenna can result in defects in the joint cuticle at the a2/a3 joint. In antenna with large homozygous clones, the internal cuticular structures are completely absent. When the aristae of these mutants are stimulated with a paintbrush or forceps, a3 does not rotate within a2.

Animals containing homozygous clones in the antenna show a lower amplitude response in the antennal nerve (similar to background noise) than wild-type flies in response to a pulse-song, indicating that they are deaf.

Scolopidia form in homozygous clones in the pupal antenna, but they are abnormal in their organisation and reduced in number compared to wild type. In adults carrying large homozygous clones in the antenna, neurons and scolopale cells are rarely seen within a2. Vacuolated degenerated cells are present in a3.

Adults carrying large homozygous clones in the antenna show an incompletely penetrant phenotype involving transformation of the arista to tarsal identity; in 8% of cases the arista is partially transformed to distal leg, while in 11% of cases the transformation is more complete and includes tarsal claws.

Homozygous ctC145 mutant clones display a marked reduction of dendrite outgrowth and branching. Dendritic termini are concentrated nearer to the cell body than in wild-type.

Lateral antennal lobe projection neurons (lPN) in ctC145 homozygous somatic clones fail to target DMA, DM2 and VA5 antennal glomeruli. Mis-targeted lPN dendrites are biased towards more lateral glomeruli than in wild-type. The number of ventral antennal projection neurons (vPN) is severely reduced in these clones and those remaining mis-target their dendrites to the subesophageal ganglion. ctScer\UAS.cPa; Scer\GAL4GH146 completely rescues the targeting of DMA, DM2 and VA5 by lPN in these animals but fails to rescue the decrease in vPN cell number and only partially rescues vPN targeting VA1lm but not to DA1.

When single cell clones are made in multidendritic neurons show a dramatic defect in higher order branching and elongation, resulting in a highly reduced dendritic field. ddaA neurons show slightly less extreme phenotypes than ddaF. ddaA do not have 'spikes'. ldaB occasionally show a severe growth phenotype, but more often show an absence or severe reduction in total dendritic length and number of branch points. A lack of spikes is also the dominant phenotype of v'pda. ddaC neurons show decreased branching and elongation, though the severity of these defects are highly variable. The most severely affected neurons extend a 'skeleton' of the normal arbor with a complete loss of higher order branches and a reduced overall dendrite length. ddaB show a severe and completely penetrant decrease in dendrite growth. ldaA and the ventral neurons show variable reductions in branching. However ddaD and ddaE show normal dendrite morphologies.

In egg chambers containing ctC145 homozygous follicle cell clones, nurse cells with two nuclei and a spot of filamentous actin containing material can be found. These binucleate cells can be found at any position and among any of the nurse cells in the egg chamber. Most of the affected egg chambers contain a single binucleate cell, although occasionally more than one binucleate cell is present. Occasionally more than two nuclei are seen in a single cell. A clone in which approximately 25-30% of follicle cells are homozygous for ctC145 is sufficient to produce an egg chamber with binucleate cells. Binucleate cells are not seen prior to stage 5 and are observable in stage 10 egg chambers as soon as 4 days after clonal induction. In egg chambers containing binucleate cells, ring canals at various stages of degeneration are seen, ranging from free-floating, detached, round ring canals to dense actin-containing spots. The total number of normal ring canals and degenerating ring canal remnants in these egg chambers is 15, suggesting that there is no net change in the number of cystoblast cytokineses.

At 18oC ctL188/ctC145 is essentially lethal, whereas at 24oC ~50% flies eclose and appear morphologically normal.

Egg chambers containing somatic clones of homozygous ctC145 cells exhibit a number of phenotypes. The homozygous clones in the follicle epithelium contain 2 to 5 times fewer cells than their corresponding wild-type twin clones. The cells in the clone and their nuclei are larger than surrounding heterozygous or wild-type cells. 44% of ovarioles studied 7 days after induction of the somatic clones contain germaria that are greatly reduced in size or absent. 4 days after induction of the somatic clones, more than 50% of stage 6 and older egg chambers contain nurse cells with multiple nuclei. These egg chambers contain at least 30% homozygous ctC145 follicle cells. 7-14 days after induction of the somatic clones, egg chambers containing abnormal numbers of germline derived nuclei are seen. This phenotype is seen in less than 1% of cases.

Convergence-extension movements in the Malpighian tubules are prevented.

Lethal in combination with ctL188.

Homozygous embryos do not form es organs (FBrf0045757). In the presence of P{hsp(sE)-poxn} embryos still do not form any detectable external sensory organs.

Embryonic muscle phenotype wild type.

Lethality occurs during embryonic and early larval stages. Transformation of es organs to ch-like, scolopales are visible and external structures are missing. ch neurons are the same as in wild type.

Expression of ctLS enhancer trap lines in a ctC145 mutant background demonstrates that some of the markers for Malpighian tubule cells alter their expression. Kr and cad expression in the tubules remains unchanged.

es organs are transformed to as ch identity, scolopales are present.

Homozygous embryos lack Malpighian tubules and the gut wall is 3--4 fold thicker than wild type at the junction of the posterior and anterior midgut.

Sense organ es to ch transformation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

ctC145 has visible | somatic clone phenotype, enhanceable by ssa

Suppressed by
Enhancer of
Statement
Reference

ct[+]/ctC145 is an enhancer of lethal | recessive | partially phenotype of mir-bft6

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

ctC145 has arista | somatic clone phenotype, enhanceable by ssa

ctC145 has leg | ectopic | somatic clone phenotype, enhanceable by ssa

ctC145 has phenotype, enhanceable by capu[+]/capuHK3

ctC145 has phenotype, enhanceable by otu11

ctC145 has egg chamber phenotype, enhanceable by Df(1)JC70/+

ctC145 has egg chamber phenotype, enhanceable by Df(1)RA2/+

ctC145 has egg chamber phenotype, enhanceable by Df(1)KA14/+

ctC145 has egg chamber phenotype, enhanceable by Df(1)N105/+

ctC145 has egg chamber phenotype, enhanceable by Df(1)JA26/+

ctC145 has egg chamber phenotype, enhanceable by Df(2L)30A-C/+

ctC145 has egg chamber phenotype, enhanceable by +/Df(2R)44CE

ctC145 has egg chamber phenotype, enhanceable by Df(2R)eve/+

ctC145 has egg chamber phenotype, enhanceable by Df(2R)or-BR6/+

ctC145 has egg chamber phenotype, enhanceable by Df(2R)M60E/+

ctC145 has egg chamber phenotype, enhanceable by Df(3L)GN50/+

ctC145 has egg chamber phenotype, enhanceable by Df(3L)GN24/+

ctC145 has egg chamber phenotype, enhanceable by Df(3L)66C-G28/+

ctC145 has egg chamber phenotype, enhanceable by Df(3L)AC1/+

ctC145 has egg chamber phenotype, enhanceable by Df(3L)W4/+

ctC145 has egg chamber phenotype, enhanceable by Df(3R)Antp17/+

ctC145 has egg chamber phenotype, enhanceable by Df(3R)P14/+

ctC145 has egg chamber phenotype, enhanceable by Df(3R)Tl-P/+

ctC145 has egg chamber phenotype, enhanceable by Df(1)otu-PΔ1/+

ctC145 has egg chamber phenotype, enhanceable by otu[+]/otu11

NOT Enhanced by
Statement
Reference

ctC145 has epidermal cell phenotype, non-enhanceable by crb8F105

ctC145 has phenotype, non-enhanceable by chic[+]/chic07886

ctC145 has phenotype, non-enhanceable by Src64BΔ17/Src64B[+]

ctC145 has phenotype, non-enhanceable by qua[+]/qua9

ctC145 has phenotype, non-enhanceable by Egfr[+]/Egfrf24

ctC145 has phenotype, non-enhanceable by Btk29A[+]/Btk29Ak00206

ctC145 has phenotype, non-enhanceable by Btk29Ak00206

ctC145 has phenotype, non-enhanceable by Egfrf24

ctC145 has phenotype, non-enhanceable by Src64BΔ17

ctC145 has phenotype, non-enhanceable by arm1

ctC145 has phenotype, non-enhanceable by arm4

ctC145 has phenotype, non-enhanceable by chic[+]/chick13321

ctC145 has phenotype, non-enhanceable by chic01320

ctC145 has phenotype, non-enhanceable by chic07886

ctC145 has phenotype, non-enhanceable by chick13321

ctC145 has phenotype, non-enhanceable by qua9

ctC145 has phenotype, non-enhanceable by spir1

ctC145 has phenotype, non-enhanceable by spir2

ctC145 has phenotype, non-enhanceable by sqh1

ctC145 has phenotype, non-enhanceable by sqh2

ctC145 has phenotype, non-enhanceable by chic[+]/chic01320

ctC145 has phenotype, non-enhanceable by sqh1/sqh[+]

ctC145 has phenotype, non-enhanceable by sqh2/sqh[+]

ctC145 has phenotype, non-enhanceable by arm1/arm[+]

ctC145 has phenotype, non-enhanceable by arm4/arm[+]

ctC145 has phenotype, non-enhanceable by spir[+]/spir1

ctC145 has phenotype, non-enhanceable by spir2/spir[+]

Suppressed by
NOT suppressed by
Statement
Reference

ctC145 has epidermal cell phenotype, non-suppressible by crb8F105

Suppressor of
Statement
Reference

ct[+]/ctC145 is a suppressor of egg chamber phenotype of Nl1N-ts1

ctC145 is a suppressor of Malpighian tubule phenotype of shgg317

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Tubules in ctC145; crb1 double-mutant embryos resemble those of ctC145 single mutants; they form epithelial blisters.

The transformation of arista to leg seen in flies carrying antennal ctC145 clones is enhanced if they also carry ssa; both the severity of the transformation and the percentage of flies showing the transformation is increased in the double mutants.

Lim1Scer\UAS.cTa; Scer\GAL4GH146 does not suppress the reduction in ventral antennal lobe projection neuron (vPN) numbers seen in ctC145/ctC145 somatic clones but does partially rescue the defects in targeting to antennal glomeruli seen in these neurons. Targeting defects in lateral antennal lobe projection neurons (lPN) in these clones are not rescued by Lim1Scer\UAS.cTa; Scer\GAL4GH146.

ctC145/+ ; bft6/bft6 animals do not survive to adulthood.

ctC145/+ ; capu1/+ and ctC145/+ ; capu2/+ double heterozygotes produce egg chambers with binucleate cells. The majority of egg chambers contain a single binucleate cell, although occasionally more than one binucleate cell per egg chamber is seen. The binucleate cells can occur anywhere among the nurse cells and contain remnants of ring canals. The binucleate cells are not seen prior to stage 5. ctC145 produces egg chambers with binucleate cells in double heterozygous combination with a number of deficiencies; Df(1)JC70, Df(1)RA2, Df(1)KA14, Df(1)N105, Df(1)JA26, Df(2L)30A-C, Df(2R)44CE, Df(2R)eve, Df(2R)or-BR6, Df(2R)M60E, Df(3L)GN50, Df(3L)GN24, Df(3L)66C-G28, Df(3L)AC1, Df(3L)W4, Df(3R)Antp17, Df(3R)P14, Df(1)otu-PΔ1 and Df(3R)Tl-P. capuHK3 does not interact with ctC145 in a doubly heterozygous background, although it does enhance the frequency of binucleate cell production in the egg chambers of ctC145/ctL188 animals. Src64BΔ17, qua9, Egfrf24, Btk29Ak00206, spir1, spir2, arm4/+, chic07886/+ or chick13321/+ do not interact with ctC145/ctL188. Dominantly enhances the wing notching phenotype seen in Df(1)N-8/+ flies; notches are deeper and more frequent. Double heterozygotes with Df(1)sd72b show a wing notching phenotype. ctC145/Df(1)A209 flies have shortened and tapered bristles. ctC145/+ ; otu11/+ egg chambers contain binucleate cells. The majority of egg chambers contain a single binucleate cell, which can be positioned anywhere in the egg chamber, although occasionally more than one binucleate cell per egg chamber is seen. ctC145/agn1 females placed at the restrictive temperature (29oC) for 5 days have egg chambers which contain binucleate cells (1-5% of all egg chambers).

63% of ctL188/ctC145; Antp11/+ flies show a humeral outgrowth phenotype at 24oC.

Egg chambers of ctL188/ctC145 females sometimes contain two diploid nuclei, and the distribution of ring canals may be abnormal. Ovaries from ctC145/+ ; RpII140wimp/+ females contain egg chambers with an abnormal number of germline cells and show cyst encapsulation defects. No multinucleate cells are seen in the egg chambers.

Malpighian tubule defect of shgg317 is rescued by ctC145.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Lefevre.

Comments
Comments

lethal II cut phenotypic class.

A cut lethal II phenotypic class allele. ctC145 failed to complement ctlS2, ctL188, ctL221 and ctL242.

Germline clonal analysis indicates that ct function is not required in the germline during oogenesis.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (48)