Homozygous clones in second leg discs fall into two categories; those that are well integrated into the disc epithelium and whose borders are interdigitated with their wild-type neighbours and those that are rounded up and have smooth borders. The interdigitated cones occur in all regions of the leg disc and appear to develop completely normally. The rounded up clones often have borders that coincide with novel folds in the disc. These rounded up clones almost always show some connection to the proximal ring that co-expresses Dll, hth and exd and are transformed to antenna (as assayed by marker expression).
The posterior signaling center is absent in Antp25/Antp17 mutant larvae.
In Antp25 embryos, thoracic neuroblast 3-3 (NB3-3T) fails to enter quiescence during mid-embryogenesis. Correspondingly, NB3-3T generates an increased number of GMCs and EL neurons compared to wild type.
Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for Antp25 show no significant defects in dendrite development.
Loss of Antp function in Antp25 mutants does not affect the NB6-4t lineage in any of the thoracic segments.
Late homozygous embryos have normal oenocyte clusters.
Thoracic BrdU incorporation associated with ventrolateral/lateral neuroblasts is normal in homozygous embryos. However, additional staining is detected in ventral positions.
Severe disorganisation of T2 and T3 muscle pattern and absence of the first midgut constriction.
Expression of nub in endoderm is wild type.
Staining with antibodies reveals that Antp25 is necessary for many thoracic es organs, it is required for lch3 axonal projection and contributes to ch organs in T2 and T3.
No salivary gland defects.
Homozygous clones in the leg show deletions of structures from the femur, tibia and proximal part of the basitarsus.
Hemizygotes lack the anterior constriction of the midgut. In hemizygotes Scr expression is very much reduced.
X ray induced somatic clones homozygous for Antp25 demonstrate that loss of Antp function results in the development of only a restricted portion of the thorax and mesothoracic legs are transformed to an antennal identity.