FlyBase Allele Report: Dmel\crb[8F105]

General Information

Symbol

Dmel\crb^8F105

Species

D. melanogaster

Name

FlyBase ID

FBal0001816

Feature type

allele

Associated gene

Dmel\crb

Associated Insertion(s)

Carried in Construct

Also Known As

crb¹, crumbs^8F105

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wodarz et al., 1993)

Mutagen

ethyl methanesulfonate

(Wodarz et al., 1993, Tepass and Knust, 1990, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Tepass and Knust, 1990)

Description

Nucleotide substitution: C6581T.

(Wodarz et al., 1993)

The premature stop codon is predicted to result in a truncated protein lacking the last 23 amino acids of the wild-type protein.

(Wodarz et al., 1993)

Amino acid replacement: Q2125term.

(Wodarz et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,313,808..24,313,808 [+]

Nucleotide change:

C24313808T

Reported nucleotide change:

C6581T

Amino acid change:

Q2124term | crb-PA; Q2167term | crb-PB; Q2231term | crb-PC; Q2181term | crb-PD

Reported amino acid change:

Q2125term

(Wodarz et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

In the crb^8F105 mutant, crb protein is observed on the basal and lateral cell membranes in addition to the apical membranes.

(Tepass et al., 1990)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Campbell et al., 2009)

abnormal mitotic cell cycle | embryonic stage

(Yeom et al., 2015)

lethal - all die during embryonic stage | recessive

(Zamudio-Arroyo and Riesgo-Escovar, 2016, Wodarz et al., 1993, Tepass and Knust, 1990, Tepass et al., 1990)

some die during embryonic stage | recessive

(Wodarz et al., 1993, Tepass and Knust, 1990, Tepass et al., 1990)

Phenotype Manifest In

adherens junction

(Mishra et al., 2012)

amnioserosa

(Grawe et al., 1996)

embryonic/larval cuticle | embryonic stage

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

embryonic/larval esophagus | embryonic stage

(Wodarz et al., 1993)

embryonic/larval hindgut | embryonic stage

(Wodarz et al., 1993)

embryonic/larval pharynx | embryonic stage

(Wodarz et al., 1993)

embryonic/larval proventriculus | embryonic stage

(Wodarz et al., 1993)

embryonic/larval salivary gland | embryonic stage

(Grawe et al., 1996, Wodarz et al., 1993)

embryonic epidermis

(Grawe et al., 1996, Wodarz et al., 1993)

embryonic Malpighian tubule

(Campbell et al., 2009, Wodarz et al., 1993)

follicle cell | somatic clone

(Penalva and Mirouse, 2012)

ocellar retinula cell

(Mishra et al., 2012)

ocellus rhabdomere

(Mishra et al., 2012)

spiracular chamber

(Wodarz et al., 1993)

spot adherens junction

(Grawe et al., 1996)

tracheal section

(Grawe et al., 1996)

zonula adherens

(Grawe et al., 1996)

Detailed Description

Statement

Reference

crb^8F105 homozygotes die as embryos with only pieces of cuticle formed.

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105 mutant two hour-old embryos show chromosome defects such as chromosome-free centrosomes, chromosomal bridges and abnormal microtubule pattern during mitosis.

(Yeom et al., 2015)

In crb^8F105 mutant ocelli, rhabdomeres are in the correct location in the retina and exhibit normal shapes, but irregular alignment, and adherens junctions do not form correctly in photoreceptor cells. Ocellar photoreceptor cells in crb^8F105 mutants do not show signs of degeneration after light exposure.

(Mishra et al., 2012)

Homozygous follicle cell clones show multilayering of cells in 42% of cases and show a flattened cells compared to wild-type cells in 22% of cases.

(Penalva and Mirouse, 2012)

crb¹ mutant embryos at stage 15 show renal tubules that fail to elongate, presenting the appearance of disorganised clusters. Ultrastructural analysis reveals that the zona adherens have dispersed by this stage.

(Campbell et al., 2009)

Homozygous clones that cover large areas of the wing can be recovered, indicating that loss of crb function does not compromise cell viability.

(Herranz et al., 2006)

When flies with eyes containing homozygous clones are kept in constant light conditions for 7 days, no retinal degeneration is seen.

(Johnson et al., 2002)

Epithelial cells of ectodermal origin lose their apicobasal polarity resulting in the loss of epithelial integrity and cell death. Both epidermal and amnioserosa cells of stage 10 lack zonula adherens junctions (ZA) and the number of spot adherens junctions (SAJ) is lower. The structure of all ectodermally derived epithelia is affected to varying extents.

(Grawe et al., 1996)

Epithelial tissues begin to degenerate from stage 11 onward in homozygous embryos. The epidermis, atrium and pharynx degenerate rapidly and almost completely, while the oesophagus, proventriculus and parts of the hindgut degenerate more slowly and to a lesser extent. Salivary glands and Malpighian tubules are reduced in size and are often dislocated.

(Wodarz et al., 1993)

Strong crb phenotype. Embryos almost completely lack cuticle due to cell death in epidermal primordium. P{lacZ}6-81 enhancer detection line specifically expresses Ecol\lacZ in the tracheal system, and reveals that morphogenetic abnormalities in the developing tracheal system result in a only a few aggregates of tracheal cells. Morphogenetic abnormalities and cell death were detected in the presumptive foregut and hindgut by fkh protein staining. Salivary glands also undergo cell death.

(Tepass and Knust, 1990)

Severe disruption in the organization of ectodermally derived epithelia, possibly cell death in the tissues.

(Tepass et al., 1990)

embryonic lethal many small holes in cuticle.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

crb^8F105 has lethal - all die during embryonic stage | recessive phenotype, suppressible by chem¹/chem[+]

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105 has lethal - all die during embryonic stage | recessive phenotype, suppressible by chem¹/chem¹

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

Enhancer of

Statement

Reference

crb^8F105 is an enhancer of visible phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

Suppressor of

Statement

Reference

crb[+]/crb^8F105 is a suppressor of lethal | recessive phenotype of chem¹

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105/crb^8F105 is a suppressor of lethal | recessive phenotype of chem¹

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

crb^8F105 is a suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

crb^8F105 is a suppressor of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

crb^8F105 has embryonic/larval cuticle | embryonic stage phenotype, suppressible by chem¹/chem[+]

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105 has embryonic/larval cuticle | embryonic stage phenotype, suppressible by chem¹/chem¹

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

Enhancer of

Statement

Reference

crb^8F105/crb^8F105 is an enhancer of embryonic/larval cuticle | embryonic stage phenotype of chem¹

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105 is an enhancer of wing phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

crb^8F105 is a non-enhancer of epidermal cell phenotype of ct^C145

(Campbell et al., 2009)

Suppressor of

Statement

Reference

crb[+]/crb^8F105 is a suppressor | partially of egg chamber phenotype of RhoGAP19D^unspecified

(Fic et al., 2021)

crb[+]/crb^8F105 is a suppressor of embryonic/larval cuticle | embryonic stage phenotype of chem¹

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

crb^8F105 is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

crb^8F105 is a suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

crb^8F105 is a suppressor of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

NOT Suppressor of

Statement

Reference

crb^8F105 is a non-suppressor of epidermal cell phenotype of ct^C145

(Campbell et al., 2009)

Other

Statement

Reference

chp[+]/chp², crb^8F105 has rhabdomere of eye photoreceptor cell phenotype

(Gurudev et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The embryonic cuticular defects and lethality observed in crb^8F105 homozygotes are weakly suppressed by combination with single copy of chem¹ and more strongly so by combination with two copies, some crb^8F105 mutant embryos heterozygous or homozygous for chem¹ reach adulthood when cultured separately without larvae of other genotypes. Conversely, crb^8F105 heterozygosity partially suppresses chem¹ mutant embryonic cuticular defects and lethality and the surviving larvae (cultured separately) reach the adult stage at the same rate as sibling double heterozygotes. The cuticular phenotype of crb^8F105;chem¹ homozygous double mutants is improved relative to crb^8F105 single mutants but worsened relative to chem¹ single homozygotes.

(Zamudio-Arroyo and Riesgo-Escovar, 2016)

The epidermis of crb¹ Kr¹ double mutant embryos exhibit relatively normal distributions of cell polarity and junctional proteins. There is some suppression of the crb¹ phenotype; a continuous, through reduced, cuticle sheet is formed.

Tubules in ct^C145; crb¹ double-mutant embryos resemble those of ct^C145 single mutants; they form epithelial blisters.

(Campbell et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

crb^8F105 is partially rescued by crb^intra.GMR

(Johnson et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7099

ru¹ hry¹ Diap1^th-1 st¹ cu¹ sr¹ e^s crb^8F105/TM3, Sb¹

Notes on Origin

Discoverer

Comments

A strong allele of crb.

(Tepass et al., 1990)

Strong crb allele.

(Tepass and Knust, 1990)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

crb¹

(Tokamov et al., 2023, Su et al., 2017, Calero-Cuenca et al., 2016, Penalva and Mirouse, 2012, Ling et al., 2010, Christensen et al., 2008.10.20, Blankenship et al., 2007, Herranz et al., 2006)

crb^8F

(Tearle and Nusslein-Volhard, 1987)

crumbs^8F105

(Zamudio-Arroyo and Riesgo-Escovar, 2016, Medina et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (33)

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