FlyBase Allele Report: Dmel\capu[1]

General Information

Symbol

Dmel\capu¹

Species

D. melanogaster

Name

FlyBase ID

FBal0001537

Feature type

allele

Associated gene

Dmel\capu

Associated Insertion(s)

Carried in Construct

Also Known As

capu^RK12, capu^RK, cappuccino^RK

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1991, Manseau and Schupbach, 1989)

Progenitor genotype

Cytology

Description

Amino acid replacement: L767H.

(Emmons et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:3,873,840..3,873,840 [-]

Nucleotide change:

T3873840A

Amino acid change:

Reported amino acid change:

L767H|FBrf0083891

Comment:

Site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change; CDS from reference sequence longer by 1 amino acid than published CDS in GB:U34258 and thus the mutation coordinates differ by 1; a sequence difference between the reference sequence and the published sequence account for the L->Q versus L->H change predicted for this mutation

(Emmons et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin filament

(Quinlan, 2013)

actin filament, with Scer\GAL4^{VP16.nanos.UTR}, capu^{I706A.UASp.RA.GFP}

(Quinlan, 2013)

dendrite | larval stage (with capu^EE)

(Stürner et al., 2022)

dendritic branch | decreased number | larval stage (with capu^EE)

(Stürner et al., 2022)

dorsal appendage

(Schupbach and Wieschaus, 1991)

dorsal appendage | maternal effect

(Manseau and Schupbach, 1989)

egg | maternal effect

(Manseau and Schupbach, 1989)

egg | maternal effect (with capu^2L-281-6)

(Luschnig et al., 2004)

egg chamber

(Quinlan, 2013, Jackson and Berg, 1999)

egg chamber | maternal effect

(Manseau and Schupbach, 1989)

egg chorion

(Emmons et al., 1995)

egg chorion | maternal effect

(Manseau and Schupbach, 1989)

embryo | blastoderm stage | maternal effect

(Manseau and Schupbach, 1989)

embryo | maternal effect

(Manseau and Schupbach, 1989)

embryonic abdomen | maternal effect

(Manseau and Schupbach, 1989)

embryonic abdominal segment

(Ephrussi et al., 1991, Schupbach and Wieschaus, 1991)

embryonic abdominal segment | maternal effect

(Raff et al., 1990)

follicle cell | maternal effect

(Manseau and Schupbach, 1989)

larval multidendritic class III neuron (with capu^EE)

(Stürner et al., 2022)

microtubule

(Emmons et al., 1995)

nurse cell ring canal

(Jackson and Berg, 1999)

oocyte

(Quinlan, 2013)

oocyte, with Scer\GAL4^{VP16.nanos.UTR}, capu^{I706A.UASp.RA.GFP}

(Quinlan, 2013)

oocyte nucleus

(Capri et al., 1997)

pole granule

(Schupbach and Wieschaus, 1991, Lasko and Ashburner, 1990)

pole granule | maternal effect

(Raff et al., 1990)

primordial germ cell

(Schupbach and Wieschaus, 1991)

primordial germ cell | maternal effect

(Manseau and Schupbach, 1989)

Detailed Description

Statement

Reference

Scer\GAL4^{nos.UTR.T:Hsim\VP16}>capu^{Scer\UAS.P\T.RA.T:Avic\GFP} expression rescues the capu¹ mutant background in that the mesh is present and streaming does not begin prematurely in 100% of egg chambers. 36% of the eggs laid by the capu¹ mutant females expressing capu^{Scer\UAS.P\T.RA.T:Avic\GFP} hatch.

56% of eggs laid by capu¹ mutant females expressing Scer\GAL4^{nos.UTR.T:Hsim\VP16}>capu^{Scer\UAS.P\T.RA} hatch.

18% of eggs laid by capu¹ mutant females expressing Scer\GAL4^{nos.UTR.T:Hsim\VP16}>capu^{L1048A.Scer\UAS.P\T.RA.T:Avic\GFP} hatch.

capu¹ mutant females expressing Scer\GAL4^{nos.UTR.T:Hsim\VP16}>capu^{I706A.Scer\UAS.P\T.RA.T:Avic\GFP} are sterile. None of their eggs hatch. No actin mesh is detectable in the mutant oocytes and streaming starts prematurely.

(Quinlan, 2013)

capu^2L-281-6/capu¹ females produce dorsalised eggs.

(Luschnig et al., 2004)

Stage 10 homozygous egg chambers contain binucleate cells. These binucleate cells are not detectable in the germarium or early stages of oogenesis. Fragments of ring canals are seen.

(Jackson and Berg, 1999)

The oocyte nucleus is still located in an anterior position in a stage 8 egg chamber in homozygous females.

(Capri et al., 1997)

40-70% of embryos laid by homozygous females have abnormal eggshells. Abnormal microtubule distribution in stage 8 and 9 egg chambers (distribution resembles that of stage 10 egg chambers), long and thick tubulin fibres are seen wrapping around the cortex of mutant oocytes. Egg chambers undergo premature microtubule based cytoplasmic streaming.

(Emmons et al., 1995)

Dorsoventral and anteroposterior patterning is disrupted.

(Cheung et al., 1992)

Variable expansion (dorsalization) of dorsal appendages, at expense of main body shell. Absence of polar granules, pole cells and abdominal segments in embryos.

(Schupbach and Wieschaus, 1991)

Homozygous embryos derived from homozygous females have no polar granules, fail to form pole cells, have deletions of abdominal structures and defects associated with dorsal/ventral pattern formation.

(Raff et al., 1990)

Homozygous mothers produce dorsalized egg shells and embryos. The first sign of dorsalization in embryos can be seen during gastrulation, when the ventral furrow is reduced or absent. Dorsalization in the egg chamber is evident in the shape of the follicle cells. While the number of follicle cells around the main body of the egg shell is reduced, dorsal appendages are expanded, often fused dorsally and/or extended ventrally. The embryos lack polar granules and pole cells, and show cellularization defects. Embryos show strong abdominal segmentation defects similar to those produced by mutations in the grandchildless-knirps or posterior class of maternal effect loci, abdominal segments are deleted.

(Manseau and Schupbach, 1989)

Homozygous femles lay eggs which sometimes (5-10%) have a 'pointed cap' (cappuccino) of dorsal appendage material sitting over the anterior end of the egg, instead of two distinct dorsal appendages. Such eggs are similar to eggs formed by the female-sterile mutation fs(1)K10 but the extent of dorsal appendage material on capu eggs is much more variable than that of fs(1)K10 eggs. No polar granules are found in such eggs. Mutant females produce embryos lacking polar granules, pole cells and normal abdominal segmentation. In combination with BicD, however, abdominal segmentation does develop in the anterior half of the embryo. Improper localization of abdominal determinants also indicated by the lack of posterior localization of vasa protein. Cellularization of the blastoderm irregularly defective, with nuclei of different sizes and densities. Resemble embryos formed by other grandchildless-knirps-like mutations, such as vas or tud, but in addition, some of the embryos from capu¹ also appear dorsalized. Mosaic studies demonstrate germ-line function of capu.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

capu¹ is an enhancer of lethal | embryonic stage | maternal effect | dominant phenotype of Rho1^rev220

(Magie et al., 1999)

Other

Statement

Reference

capu¹, spir^2F has abnormal neuroanatomy | larval stage phenotype

(Stürner et al., 2022)

Ets97D^tne-1/Df(3R)ro80b, capu[+]/capu¹ has decreased fecundity | female phenotype

(Gajewski and Schulz, 1995)

Phenotype Manifest In

Enhancer of

Statement

Reference

capu[+]/capu¹ is an enhancer of egg phenotype of Ets97D^tne-1

(Gajewski and Schulz, 1995)

NOT Enhancer of

Statement

Reference

capu[+]/capu¹ is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

NOT Suppressor of

Statement

Reference

capu[+]/capu¹ is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Other

Statement

Reference

capu¹, spir^2F has larval multidendritic class III neuron phenotype

(Stürner et al., 2022)

capu¹, spir^2F has dendrite | larval stage phenotype

(Stürner et al., 2022)

Rho1^1B, capu¹ has microtubule phenotype

(Rosales-Nieves et al., 2006)

Rho1^1B, capu¹ has oocyte | oogenesis stage S8 phenotype

(Rosales-Nieves et al., 2006)

Rho1^1B, capu[+]/capu¹ has microtubule phenotype

(Rosales-Nieves et al., 2006)

Rho1^1B, capu[+]/capu¹ has oocyte | oogenesis stage S8 phenotype

(Rosales-Nieves et al., 2006)

capu¹, ct^C145 has egg chamber phenotype

(Jackson and Berg, 1999)

capu¹, ct^C145 has nurse cell ring canal phenotype

(Jackson and Berg, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Stage 8 oocytes from capu¹; Rho1^1B and capu¹/+, spir^2F/+ mothers exhibit premature ooplasmic streaming and subcortical arrays of microtubules. Stable microtubules are restricted to the cortex, as in wild-type oocytes, but differ in the lack of reduction of microtubules at the posterior pole.

(Rosales-Nieves et al., 2006)

ct^C145/+ ; capu¹/+ double heterozygotes produce egg chambers with binucleate cells. The majority of egg chambers contain a single binucleate cell, although occasionally more than one binucleate cell per egg chamber is seen. The binucleate cells can occur anywhere among the nurse cells and contain remnants of ring canals. The binucleate cells are not seen prior to stage 5.

(Jackson and Berg, 1999)

Egg chambers transheterozygous for Rho1^rev220 and capu mutants show disruptions of the ovarian cytoskeleton and ring canals similar to that seen in females heterozygous for Rho1^rev220 and RpII140^wimp.

(Magie et al., 1999)

One copy of capu¹ decreases the number of eggs laid by Ets97D^tne-1/Df(3R)ro80b females.

(Gajewski and Schulz, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

capu¹/capu^EE is rescued by capu^UAS.mCherry/Scer\GAL4^smid-C161

(Stürner et al., 2022)

Partially rescued by

capu¹ is partially rescued by capu^UASp.RA.GFP

(Quinlan, 2013)

capu¹ is partially rescued by capu^UASp.cQa

(Quinlan, 2013)

capu¹ is partially rescued by capu^{L1048A.UASp.RA.GFP}

(Quinlan, 2013)

Not rescued by

capu¹ is not rescued by Scer\GAL4^{VP16.nanos.UTR}/capu^{I706A.UASp.RA.GFP}

(Quinlan, 2013)

Comments

56% of eggs laid by capu¹ mutant females expressing Scer\GAL4^{nos.UTR.T:Hsim\VP16}>capu^{Scer\UAS.P\T.RA} hatch.

18% of eggs laid by capu¹ mutant females expressing Scer\GAL4^{nos.UTR.T:Hsim\VP16}>capu^{L1048A.Scer\UAS.P\T.RA.T:Avic\GFP} hatch.

(Quinlan, 2013)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5094

capu¹ cn¹ bw¹/CyO, l(2)DTS513¹

Kyoto

108038

capu¹ cn¹ bw¹/CyO, l(2)DTS513¹

Notes on Origin

Discoverer

Comments

Posterior localization of vas and CycB transcripts is completely abolished.

(Raff et al., 1990)

Localization of vas protein to pole cells and polar granules abolished.

(Lasko and Ashburner, 1990)

Moderate capu allele.

(Emmons et al., 1995)

Prevents the posterior accumulation of G-iα65A protein.

(Wolfgang and Forte, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

cappuccino^RK

(Huynh et al., 2004, Jankovics et al., 2001)

capu 1

(Stürner et al., 2022)

capu(RK)

(Luschnig et al., 2004)

capu¹

(Stürner et al., 2022, Bradley et al., 2020, Pinheiro et al., 2017, Lee et al., 2016, Rudrapatna et al., 2014, Quinlan, 2013, Neuman-Silberberg, 2007)

capu^RK12

(Rosales-Nieves et al., 2006, Ji et al., 2002, Jackson and Berg, 1999, Magie et al., 1999, Capri et al., 1997, Neuman-Silberberg and Schupbach, 1996, Gajewski and Schulz, 1995, McGrail et al., 1995, Li et al., 1994, Neuman-Silberberg and Schupbach, 1993, Cheung et al., 1992, Schupbach and Wieschaus, 1991, Lasko and Ashburner, 1990, Raff et al., 1990)

capu^RK

(Dahlgaard et al., 2007, Shulman et al., 2000, Chen et al., 1997, Glotzer et al., 1997, Wilsch-Brauninger et al., 1997, Emmons et al., 1995, Rongo et al., 1995, Wolfgang and Forte, 1995, Liang et al., 1994, Ephrussi et al., 1991, St. Johnston et al., 1991, Clifford and Schupbach, 1989, Manseau and Schupbach, 1989)

Name Synonyms

Secondary FlyBase IDs

References (38)

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