FlyBase Allele Report: Dmel\chic[01320]

Germline stem cell number (GSC) is significantly reduced in chic¹¹/chic⁰¹³²⁰ mutant larval testes compared to controls. The number of GSCs is further reduced in newly eclosed adults. Somatic cells move to fill the area adjacent to the hub in the absence of GSCS.

Germline stem cell number is significantly reduced in chic²²¹/chic⁰¹³²⁰ larval testes compared to controls. In contrast to wild type testes, elongated spermatid bundles are located close to the apical tip in newly eclosed flies. Spermatogonia and spermatocytes are rare or not detected.

(Shields et al., 2014)

Mutant larvae show defects in wound closure.

(Brock et al., 2012)

Developmental changes in stress fibre orientation in chic⁰¹³²⁰ follicle cells occur as in wild type, though some defective actin organization is observed with the novel occurrence of fan-shaped stress fibres orientated perpendicular rather than parallel to the oocyte A-P axis.

(Delon and Brown, 2009)

At a stage when the first cells have just invaginated from the salivary gland placode, cell shapes within mutant placodes often appear irregular compared to wild type. At later stages, the salivary glands invaginate but are irregular in shape, and the placodal and surrounding epidermal cells on the surface of the embryos appear disrupted.

(Maybeck and Röper, 2009)

Stage 9 chic⁰¹³²⁰/chic²²¹ oocytes show premature cytoplasmic streaming.

(Dahlgaard et al., 2007)

40.5% of eggs laid by heterozygous females are short and round and have smaller and broader dorsal appendages than normal.

(Neuman-Silberberg, 2007)

The karyosomes of chic⁰¹³²⁰ mutants are abnormal in around one third of cases.

(Djagaeva et al., 2005)

8% of chic⁰¹³²⁰/chic^k13321 egg chambers have border follicle cell migration defects. 16% of chic⁰¹³²⁰/chic²²¹ egg chambers have border follicle cell migration defects.

(Geisbrecht and Montell, 2004)

Homozygous egg chambers contain binucleate cells. Remnants of ring canals are usually seen, although rarely no ring canal remnants are detectable in binucleate cells.

(Jackson and Berg, 1999)

chic^sand-1/chic⁰¹³²⁰ females fail to lay eggs.

(Wills et al., 1999)

DNA fragmentation occurs in homozygous nurse cells, but is delayed with respect to wild-type.

(Foley and Cooley, 1998)

Homozygous adults have abnormal bristles. Female fertile in transheterozygous combination with chic^8A. Onion-stage spermatids of chic⁰¹³²⁰/chic^R1 males usually contain a single Nebenkern of larger than normal size, associated with two or more normal-sized nuclei (64.3% of spermatids have 4 nuclei). Onion-stage spermatids of chic⁰¹³²⁰/chic^8A males usually contain a single Nebenkern of larger than normal size, associated with two or more normal-sized nuclei (65.0% of spermatids have 4 nuclei). 69.5% of asters show irregular positioning and delayed migration during meiotic divisions in chic⁰¹³²⁰/chic^R1 males.

(Giansanti et al., 1998)

Microtubules are bundled to the cortex of stage 8 egg chambers. Premature cytoplasmic streaming occurs in stage 8 egg chambers. Eggshells exhibit dorsal/ventral patterning defects and often show fused dorsal appendages.

(Manseau et al., 1996)

Homozygotes show extra macrochaetae. The egg chambers of chic⁰¹³²⁰/chic²²¹ and chic⁰¹³²⁰/Df(2L)GpdhA females usually have too few nurse cells that are mostly binucleate. The variation in nurse cell number is great, with some chambers having as many as 30. 10% of egg chambers show a failure of border cell migration.

(Verheyen and Cooley, 1994)

Fully penetrant egg chamber phenotype: nurse cells fail to deliver all their cytoplasm to the oocyte resulting in small oocytes surrounded by an egg shell. Strong allele of chic.

(Cooley et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

chic⁰¹³²⁰/chic²²¹ has female sterile phenotype, suppressible by Scer\GAL4^nanos.PU/Atg9^UASp.GFP

(Kiss et al., 2020)

chic⁰¹³²⁰/chic²²¹ has decreased fecundity | female phenotype, suppressible | partially by Scer\GAL4^nanos.PU/Atg9^UASp.GFP

(Kiss et al., 2020)

chic⁰¹³²⁰/chic¹¹ has decreased cell number phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP

(Shields et al., 2014)

NOT suppressed by

Statement

Reference

chic⁰¹³²⁰/chic²²¹ has decreased cell number phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP

(Shields et al., 2014)

Enhancer of

Statement

Reference

chic⁰¹³²⁰ is an enhancer of abnormal cell polarity phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

NOT Enhancer of

Statement

Reference

chic[+]/chic⁰¹³²⁰ is a non-enhancer of lethal | embryonic stage | recessive | partially phenotype of peb³⁰⁸

(Wilk et al., 2004)

chic⁰¹³²⁰ is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, msn^EP549

(Ruan et al., 2002)

Suppressor of

Statement

Reference

chic[+]/chic⁰¹³²⁰ is a suppressor of abnormal neuroanatomy phenotype of Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

chic[+]/chic⁰¹³²⁰ is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

chic[+]/chic⁰¹³²⁰ is a non-suppressor of lethal | recessive | partially | embryonic stage phenotype of peb³⁰⁸

(Wilk et al., 2004)

chic⁰¹³²⁰ is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, msn^EP549

(Ruan et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

chic⁰¹³²⁰/chic^k13321 has border follicle cell phenotype, enhanceable by Diap1⁵/th[+]

(Geisbrecht and Montell, 2004)

chic⁰¹³²⁰/chic²²¹ has border follicle cell phenotype, enhanceable by Diap1^21-2s/th[+]

(Geisbrecht and Montell, 2004)

chic⁰¹³²⁰/chic²²¹ has border follicle cell phenotype, enhanceable by Diap1⁵/th[+]

(Geisbrecht and Montell, 2004)

chic⁰¹³²⁰/chic^k13321 has border follicle cell phenotype, enhanceable by Diap1^21-2s/th[+]

(Geisbrecht and Montell, 2004)

chic⁰¹³²⁰ has phenotype, enhanceable by capu²

(Manseau et al., 1996)

chic⁰¹³²⁰ has phenotype, enhanceable by capu^2F

(Manseau et al., 1996)

Suppressed by

Statement

Reference

chic⁰¹³²⁰/chic¹¹ has male germline stem cell phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP

(Shields et al., 2014)

chic⁰¹³²⁰ has dorsal appendage phenotype, suppressible by spoon¹/spoon[+]

(Neuman-Silberberg, 2007)

chic⁰¹³²⁰ has egg phenotype, suppressible by spoon¹/spoon[+]

(Neuman-Silberberg, 2007)

NOT suppressed by

Statement

Reference

chic⁰¹³²⁰/chic¹¹ has male germline stem cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/shg^UAS.sgGFP

(Shields et al., 2014)

chic⁰¹³²⁰/chic²²¹ has male germline stem cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.GFP

(Shields et al., 2014)

chic⁰¹³²⁰/chic²²¹ has male germline stem cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/shg^UAS.sgGFP

(Shields et al., 2014)

chic⁰¹³²⁰/chic²²¹ has oocyte | oogenesis stage S9 phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP

(Dahlgaard et al., 2007)

Enhancer of

Statement

Reference

chic⁰¹³²⁰ is an enhancer of ommatidium phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

NOT Enhancer of

Statement

Reference

chic⁰¹³²⁰ is a non-enhancer of photoreceptor cell & axon phenotype of Scer\GAL4^GMR.PF, msn^EP549

(Ruan et al., 2002)

chic[+]/chic⁰¹³²⁰ is a non-enhancer of phenotype of ct^C145

(Jackson and Berg, 1999)

chic⁰¹³²⁰ is a non-enhancer of phenotype of ct^C145

(Jackson and Berg, 1999)

chic⁰¹³²⁰ is a non-enhancer of phenotype of Rho1^rev220

(Magie et al., 1999)

Suppressor of

Statement

Reference

chic[+]/chic⁰¹³²⁰ is a suppressor of ventral adult lateral neuron & axon phenotype of Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

chic[+]/chic⁰¹³²⁰ is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

chic⁰¹³²⁰ is a non-suppressor of photoreceptor cell & axon phenotype of Scer\GAL4^GMR.PF, msn^EP549

(Ruan et al., 2002)

chic⁰¹³²⁰ is a non-suppressor of phenotype of Rho1^rev220

(Magie et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} in germ cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the germline stem cell (GSC) loss seen in chic¹¹/chic⁰¹³²⁰ mutant testes.

Expression of Apc2^{Scer\UAS.T:Avic\GFP} in germ cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the germline stem cell (GSC) loss seen in chic¹¹/chic⁰¹³²⁰ mutant testes.

Expression of Apc2^{Scer\UAS.T:Avic\GFP} in germ cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the germline stem cell (GSC) loss seen in chic²²¹/chic⁰¹³²⁰ mutant testes.

(Shields et al., 2014)

A chic⁰¹³²⁰ heterozygous background partially suppresses the rough eye phenotype seen in Fmr1^sev.PW mutants.

(Estes et al., 2008)

Expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the premature cytoplasmic streaming seen in stage 9 chic⁰¹³²⁰/chic²²¹ oocytes.

(Dahlgaard et al., 2007)

Only 13.4% of eggs laid by chic⁰¹³²⁰/+ spoon¹/+ double heterozygous females have a mutant phenotype.

(Neuman-Silberberg, 2007)

80% of chic⁰¹³²⁰/chic^k13321 ; th⁵/+ egg chambers have border follicle cell migration defects. 51% of chic⁰¹³²⁰/chic^k13321 ; th^21-2s/+ egg chambers have border follicle cell migration defects. In some egg chambers, the border follicle cells migrate to the side of the egg chamber instead of through the middle of the nurse cells, a phenotype that is never seen in wild-type egg chambers. 77% of chic⁰¹³²⁰/chic²²¹ ; th⁵/+ egg chambers have border follicle cell migration defects. 48% of chic⁰¹³²⁰/chic²²¹ ; th^21-2s/+ egg chambers have border follicle cell migration defects.

(Geisbrecht and Montell, 2004)

S^48-5/chic⁰¹³²⁰ mutants show 21.90%+-5.89 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone.

(Gaengel and Mlodzik, 2003)

The phenotype caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF is not modified by the addition of chic⁰¹³²⁰.

(Ruan et al., 2002)

6% of the eggs from capu²/capu^2F and 30% from chic⁰¹³²⁰/chic⁰¹³²⁰ have fused dorsal appendages, whereas 76% of those from capu², chic⁰¹³²⁰/capu^2F, chic⁰¹³²⁰ have dorsal/ventral defects. Similarly capu^2F, chic⁰¹³²⁰/capu^2F, chic⁰¹³²⁰ produce 70% eggs with dorsal/ventral defects while capu^2F/capu^2F alone produced only 2%.

(Manseau et al., 1996)

Xenogenetic Interactions

Statement

Reference

Heterozygosity for chic⁰¹³²⁰ suppresses the Scer\GAL4^P2.4.Pdf>Hsap\APP^{Scer\UAS.T:Hsap\MYC}-induced increase in axonal arborization.

(Leyssen et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4891

P{PZ}chic⁰¹³²⁰ cn¹/CyO; ry⁵⁰⁶

Kyoto

107931

chic⁰¹³²⁰ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

chi¹³²⁰

chic-01320

(Gaengel and Mlodzik, 2003)

chic⁰¹³²⁰

(Kiss et al., 2020, Thuveson et al., 2019, Ogienko et al., 2018, Brock et al., 2012, Maybeck and Röper, 2009, Estes et al., 2008)

chic¹³²⁰

(Grintsevich et al., 2021, Shields et al., 2014, Urwyler et al., 2012, Dahlgaard et al., 2007, Neuman-Silberberg, 2007, Ruan et al., 2002, Jackson and Berg, 1999, Magie et al., 1999)

chickadee⁰¹³²⁰

(Otani et al., 2011)

fs(2)01320

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (30)

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