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General Information
Symbol
P{PZ}
FlyBase ID
FBtp0000210
Feature type
transgenic_transposon
Component Allele(s)
Size
14.545
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
lacZ enhancer trap
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description

P{PZ} is an enhancer trap construct that encodes a lacZ reporter. The enhancer trap sequence consists of the weak P\T promoter and coding sequence for the first 125 P\T amino acids fused upstream of the lacZ coding sequence.

The lacZ reporter encoded by the P{PZ} construct is expected to be localized to the nucleus, because it is fused to the N-terminal 125 amino acid residues of P\T (which have been shown to be sufficient for nuclear localization).

A diagram of the P{PZ} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

Component allele l(3)87Df7.2
Description

Construct: Portion of l(3)87Df containing an intron of 70 nucleotides, the splice acceptor site and the remaining coding sequence and transcription termination sequences.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size

14.545

Right end
 
Features
CV term
Qualifiers and info
Reference
bacterial
selection
Component Alleles
Molecular data

Construct: Portion of l(3)87Df containing an intron of 70 nucleotides, the splice acceptor site and the remaining coding sequence and transcription termination sequences.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

sequence comment:

report comment: Thanks to Brian Calvi and Vladimir Benes for assistance in assembling compiled sequence. Included as non-attributed correction.

sequence comment: Included as non-attributed correction.

mapping_comment:

report comment:

mapping_comment: Included as non-attributed correction.

construct_comment: Restriction site useful for plasmid rescue: XbaI.

Stocks (539)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (53)