FlyBase Allele Report: Dmel\capu[ΔN.UASp.GFP]

General Information

Symbol

Dmel\capu^ΔN.UASp.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0266084

Feature type

allele

Associated gene

Dmel\capu

Associated Insertion(s)

Carried in Construct

P{UASp-capu.GFP.ΔN}

Also Known As

GFP-CapuΔN

Key Links

Transgenic product class

inframe_deletion

(Dahlgaard et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Dahlgaard et al., 2007)

Mutagen

in vitro construct

(Dahlgaard et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-capu.GFP.ΔN}

Cytology

Description

UASp regulatory sequences drive expression of capu amino acid residues 271-1059 which are tagged with GFP.

(Dahlgaard et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

capu

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP is a suppressor of oocyte | oogenesis stage S9 phenotype of spir¹

(Dahlgaard et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP is a suppressor of filamentous actin | oogenesis stage S9 phenotype of spir¹

(Dahlgaard et al., 2007)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP is a non-suppressor of embryonic head phenotype of mira^{αTub67C.mGFP6}

(Chang et al., 2011)

Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP is a non-suppressor of oocyte | oogenesis stage S9 phenotype of chic⁰¹³²⁰/chic²²¹

(Dahlgaard et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is not suppressed if the females are also expressing capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Chang et al., 2011)

Expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the premature cytoplasmic streaming seen in spir¹ oocytes until the end of stage 9. The microtubules show a nearly wild-type organisation at stage 9 in the rescued oocytes, but this rescue is temporary as at stage 10A most of the oocytes show cortical arrays of microtubules.

Expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the premature cytoplasmic streaming seen in stage 9 chic⁰¹³²⁰/chic²²¹ oocytes.

The loss of the actin mesh in the cytoplasm of stage 9 spir¹ oocytes is rescued by expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, although the mesh appears less dense than in wild type. Ectopic actin mesh-like structures are also induced in the nurse cells in these animals.

(Dahlgaard et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP rescues Df(2L)ed1/capu²

(Dahlgaard et al., 2007)

Comments

Expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the premature cytoplasmic streaming seen in stage 9 capu²/Df(2L)ed1 oocytes and restores normal microtubule organisation.

The loss of the actin mesh in the cytoplasm of capu²/Df(2L)ed1 oocytes is rescued up to stage 10B by expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. Ectopic actin mesh-like structures are also induced in the nurse cells in these animals.

(Dahlgaard et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP}

capu^ΔN.UASp.GFP

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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