FlyBase Allele Report: Dmel\capu[2]

General Information

Symbol

Dmel\capu²

Species

D. melanogaster

Name

FlyBase ID

FBal0001538

Feature type

allele

Associated gene

Dmel\capu

Associated Insertion(s)

Carried in Construct

Also Known As

capu^G7

Key Links

Allele class

gain of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

gain of function allele

(Manseau et al., 1996)

Mutagen

ethyl methanesulfonate

(Manseau and Schupbach, 1989)

Progenitor genotype

Cytology

Description

Missense mutation.

(Emmons et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | maternal effect | recessive

(Manseau et al., 1996, Manseau and Schupbach, 1989)

Phenotype Manifest In

dorsal appendage

(Manseau et al., 1996)

dorsal appendage | maternal effect

(Manseau and Schupbach, 1989)

egg | maternal effect

(Manseau and Schupbach, 1989)

egg chamber

(Jackson and Berg, 1999)

egg chamber | maternal effect

(Manseau and Schupbach, 1989)

egg chorion

(Emmons et al., 1995)

egg chorion | maternal effect

(Manseau and Schupbach, 1989)

embryo | blastoderm stage | maternal effect

(Manseau and Schupbach, 1989)

embryo | maternal effect

(Manseau and Schupbach, 1989)

embryonic abdomen | maternal effect

(Manseau and Schupbach, 1989)

embryonic abdominal segment

(Smith et al., 1992)

filamentous actin | oogenesis stage S9 (with Df(2L)ed1)

(Dahlgaard et al., 2007)

follicle cell | maternal effect

(Manseau and Schupbach, 1989)

microtubule

(Emmons et al., 1995)

nurse cell ring canal

(Jackson and Berg, 1999)

oocyte | oogenesis stage S9 (with Df(2L)ed1)

(Dahlgaard et al., 2007)

primordial germ cell | maternal effect

(Manseau and Schupbach, 1989)

stretch follicle cell

(Manseau et al., 1996)

Detailed Description

Statement

Reference

Stage 9 capu²/Df(2L)ed1 oocytes show premature cytoplasmic streaming and show abnormal microtubule distribution.

Stage 9 capu²/Df(2L)ed1 oocytes do not contain the actin mesh (a uniform network of actin filaments present throughout the cytoplasm) that is seen in wild-type oocytes at this stage.

(Dahlgaard et al., 2007)

Homozygous egg chambers contain binucleate cells. Fragments of ring canals are seen.

(Jackson and Berg, 1999)

Greater than 75% of embryos laid by homozygous females are ventralised. Abnormal microtubule distribution in stage 8 and 9 egg chambers (distribution resembles that of stage 10 egg chambers), long and thick tubulin fibres are seen wrapping around the cortex of mutant oocytes. Egg chambers undergo premature microtubule based cytoplasmic streaming.

(Emmons et al., 1995)

Disrupt posterior and dorsoventral patterning in embryos. Disrupt organisation of developing microtubules during stages 8 to 10. Developmental onset of microtubule-dependent ooplasmic streaming is affected causing premature ooplasmic streaming in the mutant ovaries. The progress and termination of streaming is unaffected.

(Theurkauf, 1994)

capu²;6xP{osk⁺108} embryos exhibit a class of embryos with abdominal deletions typical of posterior group mutations.

(Smith et al., 1992)

Homozygous mothers produce dorsalized egg shells and embryos. The first sign of dorsalization in embryos can be seen during gastrulation, when the ventral furrow is reduced or absent. Dorsalization in the egg chamber is evident in the shape of the follicle cells. While the number of follicle cells around the main body of the egg shell is reduced, dorsal appendages are expanded, often fused dorsally and/or extended ventrally. The embryos lack polar granules and pole cells, and show cellularization defects. Embryos show abdominal segmentation defects similar to those produced by mutations in the grandchildless-knirps or posterior class of maternal effect loci.

(Manseau and Schupbach, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

capu² is an enhancer of lethal | embryonic stage | maternal effect | dominant phenotype of Rho1^rev220

(Magie et al., 1999)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Df(2L)ed1/capu² has oocyte | oogenesis stage S9 phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/spir^RD.UASp.GFP

(Dahlgaard et al., 2007)

Df(2L)ed1/capu² has filamentous actin | oogenesis stage S9 phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/spir^RD.UASp.GFP

(Dahlgaard et al., 2007)

Enhancer of

Statement

Reference

capu² is an enhancer of phenotype of chic⁰¹³²⁰

(Manseau et al., 1996)

NOT Suppressor of

Statement

Reference

capu[+]/capu² is a non-suppressor of embryonic head phenotype of mira^{αTub67C.mGFP6}

(Chang et al., 2011)

Other

Statement

Reference

capu², ct^C145 has egg chamber phenotype

(Jackson and Berg, 1999)

capu², ct^C145 has nurse cell ring canal phenotype

(Jackson and Berg, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The bicaudal phenotype of embryos derived from mira^{αTub67C.T:Avic\GFP-m6}/+ females is not suppressed if the females are also heterozygous for capu².

(Chang et al., 2011)

Expression of spir^{RD.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not rescue the premature cytoplasmic streaming and abnormal microtubule distribution seen in stage 9 capu²/Df(2L)ed1 oocytes.

The loss of the actin mesh in the cytoplasm of stage 9 capu²/Df(2L)ed1 oocytes is not rescued by expression of spir^{RD.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Dahlgaard et al., 2007)

ct^C145/+ ; capu²/+ double heterozygotes produce egg chambers with binucleate cells. The majority of egg chambers contain a single binucleate cell, although occasionally more than one binucleate cell per egg chamber is seen. The binucleate cells can occur anywhere among the nurse cells and contain remnants of ring canals. The binucleate cells are not seen prior to stage 5.

(Jackson and Berg, 1999)

Egg chambers transheterozygous for Rho1^rev220 and capu mutants show disruptions of the ovarian cytoskeleton and ring canals similar to that seen in females heterozygous for Rho1^rev220 and RpII140^wimp.

(Magie et al., 1999)

6% of the eggs from capu²/capu^2F and 30% from chic⁰¹³²⁰/chic⁰¹³²⁰ have fused dorsal appendages, whereas 76% of those from capu², chic⁰¹³²⁰/capu^2F, chic⁰¹³²⁰ have dorsal/ventral defects. Egg chambers show multinucleate nurse cells, due to a failure in cytokinesis.

(Manseau et al., 1996)

Injection of nos^N5 RNA into hemizygous embryos completely rescues the abdominal phenotype.

(Wang and Lehmann, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2L)ed1/capu² is rescued by capu^UASp.cDa.GFP/Scer\GAL4^{VP16.nanos.UTR}

(Dahlgaard et al., 2007)

Df(2L)ed1/capu² is rescued by Scer\GAL4^{VP16.nanos.UTR}/capu^ΔN.UASp.GFP

(Dahlgaard et al., 2007)

Comments

Expression of either capu^{Scer\UAS.P\T.cDa.T:Avic\GFP} or capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the premature cytoplasmic streaming seen in capu²/Df(2L)ed1 oocytes and restores normal microtubule organisation.

The loss of the actin mesh in the cytoplasm of capu²/Df(2L)ed1 oocytes is rescued up to stage 10B by expression of capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. Ectopic actin mesh-like structures are also induced in the nurse cells in these animals.

The loss of the actin mesh in the cytoplasm of stage 9 capu²/Df(2L)ed1 oocytes is rescued by expression of capu^{Scer\UAS.P\T.cDa} or capu^{Scer\UAS.P\T.cDa.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Dahlgaard et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8799

capu² cn¹ bw¹/CyO

Notes on Origin

Discoverer

Comments

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Although tud protein is present in mutant embryo extracts, its localization in the embryo is altered.

(Bardsley et al., 1993)

Transcripts from osk^olc1 behave as those of the endogenous osk gene.

(Kim-Ha et al., 1993)

Mutation does not affect posterior follicle cell determination.

(Gonzalez-Reyes et al., 1995)

Moderate to strong capu allele.

(Emmons et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (25)

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