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General Information
Symbol
Dmel\msnEP549
Species
D. melanogaster
Name
FlyBase ID
FBal0088791
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP(3)549, ep(3)0549
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{EP} element is inserted 20 nucleotides 5' to the predicted ATG start codon.

The P{EP} insertion is located 24bp upstream of the 5' end of a msn cDNA.

P{EP} insertion in the 5' promoter region.

Insertion components
P{EP}msnEP549
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression via Scer\GAL4fkh.PH results in a weakly penetrant 'too wide and lumpy lumen' phenotype in the embryonic salivary gland.

Expression of msnEP549 under the control of Scer\GAL4GMR.PF has no affect on the eye.

In larvae expressing msnEP549 under the control of Scer\GAL4GMR.PF, nay R1-R6 growth cones terminate before they reach the lamina plexus. The regular array of R7 and R8 growth cones in the medulla is also disorganised. This phenotype is fully penetrant.

Mutant larvae expressing msnEP549 under the control of Scer\GAL4elav-C155 have ISN neuron pathfinding defects.

msnEP549 flies, when driven by Scer\GAL4ap-md544 show abnormal orientation of the microchaetae on the notum, wing hair polarity defects are also seen, along with duplication or triplication of some hairs. Over expression of msnEP549 in the eye driven by Scer\GAL4hs.2sev causes a rough eye phenotype and reveal typical polarity defects in ommatidia. Also some ommatidia have missing or malformed photoreceptors.

Expression of msnEP549 under the control of Scer\GAL4GMR.PF causes a large number of R1-R6 photoreceptor cell growth cones to stop before they reach their normal target lamina. Defects are also seen in the medulla terminal field. The shape of the photoreceptor cells and their localisation on the developing eye disc is unaffected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, enhanceable by Cdc42unspecified

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, enhanceable by NrtM54

NOT Enhanced by
Statement
Reference

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-enhanceable by Rho1E3.10

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-enhanceable by bsk1/bsk[+]

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-enhanceable by chic01320

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-enhanceable by Pakunspecified

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-enhanceable by Rac1J11/Rac2Δ/MtlΔ

Scer\GAL4hs.2sev, msnEP549 has ommatidium phenotype, non-enhanceable by dock3

Suppressed by
Statement
Reference

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, suppressible by bif[+]/bifR47

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, suppressible by bifR38/bif[+]

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has eye photoreceptor cell & axon phenotype, suppressible by Scer\GAL4GMR.PF/Scer\GAL4GMR.PF/dockUAS.cRa

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has eye photoreceptor cell & axon phenotype, suppressible by dockW225K.UAS/Scer\GAL4GMR.PF/Scer\GAL4GMR.PF

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has eye photoreceptor cell & axon phenotype, suppressible | partially by dockW48K.UAS/Scer\GAL4GMR.PF/Scer\GAL4GMR.PF

NOT suppressed by
Statement
Reference

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-suppressible by Rac1J11/Rac2Δ/MtlΔ

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-suppressible by Rho1E3.10

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-suppressible by bsk1/bsk[+]

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-suppressible by chic01320

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has photoreceptor cell & axon phenotype, non-suppressible by Pakunspecified

Scer\GAL4hs.2sev, msnEP549 has ommatidium phenotype, non-suppressible by dock3

Scer\GAL4hs.2sev, msnEP549 has ommatidium phenotype, non-suppressible by Rho172O

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has eye photoreceptor cell & axon phenotype, non-suppressible by dockW151K.UAS/Scer\GAL4GMR.PF/Scer\GAL4GMR.PF

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549 has eye photoreceptor cell & axon phenotype, non-suppressible by Scer\GAL4GMR.PF/Scer\GAL4GMR.PF/dockR336Q.UAS

Enhancer of
Suppressor of
Statement
Reference

msnEP549, Scer\GAL4GMR.PF, Scer\GAL4GMR.PF is a suppressor of eye photoreceptor cell & axon phenotype of dock04723

msnEP549, Scer\GAL4GMR.PF, Scer\GAL4GMR.PF is a suppressor of eye photoreceptor cell & growth cone phenotype of dock04723

Additional Comments
Genetic Interactions
Statement
Reference

The defects in photoreceptor cell projection patterns seen in larvae overexpressing msnEP549 under the control of Scer\GAL4GMR.PF are largely suppressed by one copy of bifR38 or one copy of bifR47. The defects in photoreceptor cell projection patterns seen in larvae overexpressing msnEP549 under the control of Scer\GAL4GMR.PF are not affected by one copy of bsk1. The defects in photoreceptor cell projection patterns seen in larvae overexpressing msnEP549 under the control of Scer\GAL4GMR.PF are enhanced by Cdc42unspecified or NrtM54. The phenotype caused by expression of msnEP549 under the control of Scer\GAL4GMR.PF is not modified by the addition of Pakunspecified, Rho1E3.10 or chic01320. The phenotype caused by expression of msnEP549 under the control of Scer\GAL4GMR.PF is not modified by the addition of the triple mutant combination Rac1J11 Rac2Δ MtlΔ.

The phenotypes caused by over-expression of msnEP549 in the eye driven by Scer\GAL4hs.2sev are dominantly suppressed by bsk2, hepr75, JraIA109, Df(1)H6, Df(1)G24, Df(2L)flp170B, Df(3R)crb87-4, Df(2L)b80e3, Df(3R)p13, Df(3R)crb-F89-4 and Df(2L)b87e25, leading to almost wild-type ommatidial arrays. The addition of dock3, Df(3L)Ar14-8 and Rho172O have no effect.

Co-expression of dockScer\UAS.cRa or dockW225K.Scer\UAS suppresses the pretarget termination phenotype of photoreceptor cell axons caused by expression of msnEP549 under the control of Scer\GAL4GMR.PF. Co-expression of dockW48K.Scer\UAS weakly suppresses the pretarget termination phenotype of photoreceptor cell axons caused by expression of msnEP549 under the control of Scer\GAL4GMR.PF. Co-expression of dockW151K.Scer\UAS or dockR336Q.Scer\UAS does not suppress the pretarget termination phenotype of photoreceptor cell axons caused by expression of msnEP549 under the control of Scer\GAL4GMR.PF. The dock04723 phenotype is largely suppressed by msnEP549 expressed under the control of Scer\GAL4GMR.PF. The premature termination of many photoreceptor cell growth cones within the optic stalk is also seen in these larvae (this phenotype is not seen in larvae expressing msnEP549 under the control of Scer\GAL4GMR.PF in a wild-type background).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: an insertion line that modifies the eye phenotype caused by expression of Ppt1Scer\UAS.cKa under the control of Scer\GAL4GMR.PF when co-expressed using Scer\GAL4GMR.PF.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (11)