G10248232A
G?A
G225E | bsk-PB; G225E | bsk-PE; G225E | bsk-PF
G225E
Amino acid replacement: G225E.
Mutation is located between subdomains IX and X of the bsk kinase, is conserved in the MAP kinase family and is located in a region required for substrate recognition by JNK protein kinases.
Nucleotide substitution: G?A.
bsk1 heterozygotes do not show obvious thorax defects.
While females with bsk1 germline clones produced similar amount of eggs as controls, ovaries show decreased mtDNA replication in region 2B and 3 cysts, and eggs show severely decreased levels of mtDNA.
bsk1 heterozygosity does not significantly affect the mitotic index in the adult midgut epithelium, as compared to controls.
bsk1/+ third instar larvae do not show any decrease in the neuromuscular junction bouton number.
bsk1 heterozygous mutants exhibit extreme sensitivity to 1-octen-3-ol. These flies survive for approximately 15 days when exposed to 0.5ppm 1-octen-3-ol, significantly lower than the 18 days-long survival span observed for exposed wild-type flies.
As in wild type, a bsk1 mutant neuroblast clone in the larval brain maintains a single neuroblast and consists of many neuronal progeny.
Single cell bsk1 clones in mushroom bodies have axons with wild type projections.
Approximately 60% of bsk1 homozygous embryos shows a 'dorsal open' phenotype.
Mutant embryos have dorsal holes in the cuticle.
Homozygous embryos show a weak dorsal-open phenotype.
Homozygotes show a defect in dorsal closure.
Homozygous embryos show defects in dorsal closure.
Embryos exhibit a dorsal open phenotype.
Embryonic cuticle is partially closed in the dorsal posterior part. Removal of maternal and zygotic bsk (bsk1/Df(2L)flp147E) results in a complete dorsal open phenotype.
Initial phases of dorsal closure are normal, but the first rows under the leading cell row of the dorsal edge show only a partial lengthening cell shape change or no cell shape change at all. The defect is more pronounced in anterior cells. Embryonic cuticle defect is temperature sensitive: At 18oC egg lays show weaker phenotypes. Embryos derived from germline clones show an extreme dorsal open phenotype, with no cell shape change in leading row cells, and dorsal closure never initiates. This phenotype is rescuable by zygotic bsk+. Homozygous clones in the eye reveal no abnormality.
Mutant embryos show a dorsal hole due to a defect in dorsal closure. Dorsoventral patterning is not defective.
bsk1 has lethal phenotype, non-suppressible by acal[+]/lncRNA:acal5
bsk1 has some die during embryonic stage phenotype, non-suppressible by acal[+]/lncRNA:acal5
bsk1/bsk[+] is an enhancer of visible | adult stage phenotype of Scer\GAL4pnr.PU, licGD7546
bsk1 is an enhancer of visible phenotype of Scer\GAL4GMR.PF, cindrdsRNA.PC.PD.UAS
bsk1, BacA\p35GMR.PH, bsk[+] is an enhancer of increased cell death phenotype of BacA\p35GMR.PH, Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+] is an enhancer of partially lethal - majority die phenotype of slprBS06
bsk1/bsk[+] is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549
bsk1/bsk[+] is a suppressor | partially of increased cell death | larval stage phenotype of RpS3Plac92, ykiB5
bsk1/bsk[+] is a suppressor | partially of visible | adult stage phenotype of RpS3Plac92, ykiB5
bsk1/bsk[+] is a suppressor | partially of decreased size | adult stage phenotype of RpS3Plac92, ykiB5
bsk1 is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Tace1
bsk1 is a suppressor of decreased cell number | adult stage | progressive phenotype of Tace1
bsk1/bsk[+] is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4NP5130, UvragHMS01357
bsk1 is a suppressor | partially of lethal phenotype of lncRNA:acal5
bsk1 is a suppressor | partially of some die during embryonic stage phenotype of lncRNA:acal5
bsk1/bsk[+] is a suppressor | partially of abnormal size phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hepCA.UAS
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hepCA.UAS
bsk1/bsk[+] is a suppressor | partially of abnormal size phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrUAS.cIa
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrUAS.cIa
bsk1/bsk[+] is a suppressor of abnormal neurophysiology phenotype of ninaE17, ninaEP37H
bsk1/bsk[+] is a suppressor of abnormal neuroanatomy phenotype of ninaE17, ninaEP37H
bsk1 is a suppressor | partially of abnormal neuroanatomy | somatic clone | larval stage phenotype of fzy5032
bsk1/bsk[+] is a suppressor of abnormal cell migration phenotype of CskGD9345, Scer\GAL4ptc-559.1
bsk1 is a suppressor of visible phenotype of DaxxEY09290, Scer\GAL4Bx-MS1096
bsk1/bsk[+] is a suppressor of increased cell death phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/hepr75 is a suppressor of increased cell death phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+] is a suppressor of increased cell death | larval stage phenotype of Hsap\APPAβ42.UAS.cUa, Scer\GAL4elav.PU
bsk1/bsk[+] is a suppressor | partially of lethal phenotype of Hsap\APPAβ42.UAS.cUa, Scer\GAL4elav.PU
bsk1/bsk[+] is a suppressor of abnormal locomotor behavior phenotype of Hsap\APPAβ42.UAS.cUa, Scer\GAL4elav.PU
bsk1/bsk[+] is a suppressor | partially of visible phenotype of HIV-1\VpuUAS.cLa, Scer\GAL4dpp.blk1
bsk1/bsk[+] is a suppressor | partially of chemical resistant phenotype of Scer\GAL4h.PU, Tn\neoRUAS.GFP
bsk1/bsk[+] is a suppressor | partially of visible phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS
bsk1/bsk[+] is a suppressor of visible phenotype of flw1, pucA251.1F3/puc[+]
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Scer\GAL4hs.2sev, Tak1UAS.cMa
bsk1/bsk[+] is a suppressor | partially of visible phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca-537.4
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrUAS.cMa
bsk1 is a suppressor of visible phenotype of HScer\UAS.cMa/HUAS.cMa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
bsk1 is a suppressor of visible phenotype of RetMEN2B.GMR
bsk1 is a suppressor of visible phenotype of RetMEN2A.GMR
bsk1 is a suppressor of lethal | pupal stage phenotype of Cskj1D8/CskS030003
bsk1 is a suppressor of increased occurrence of cell division phenotype of Cskj1D8/CskS030003
bsk1/bsk[+] is a suppressor of visible phenotype of Scer\GAL469B, nmoc5-1.UAS
bsk1/bsk[+] is a suppressor | partially of abnormal planar polarity phenotype of peb1
bsk1/bsk[+] is a suppressor of visible phenotype of Rac1GMR.PN
bsk1 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, fzUAS.cAa
bsk1/bsk[+] is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549
Trpml1, bsk1/bsk[+] has abnormal neuroanatomy | third instar larval stage phenotype
bsk1 has embryonic dorsal epidermis phenotype, enhanceable by Cka05836/Cka05836
bsk1 has embryonic/first instar larval cuticle | dorsal phenotype, enhanceable by msn[+]/msn102
bsk1 has embryonic epidermis | dorsal phenotype, enhanceable by JrabZIP.hs.sev.Tag:HA
bsk1 has embryonic/first instar larval cuticle | dorsal phenotype, suppressible | partially by icsBG02577/icsBG02577
bsk1 has embryonic/first instar larval cuticle | dorsal phenotype, suppressible | partially by Sac1[+]/Sac1L2F
bsk1 has embryonic epidermis | dorsal phenotype, suppressible by JraAsp.hs.sev
bsk1/Df(2L)flp147E has embryonic epidermis | dorsal phenotype, suppressible by aopunspecified
bsk1/bsk[+] is an enhancer of tergum phenotype of Scer\GAL4pnr.PU, licGD7546
bsk1 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, cindrdsRNA.PC.PD.UAS
bsk1/bsk[+] is an enhancer of wing margin bristle phenotype of Pknk06808
bsk1, BacA\p35GMR.PH, bsk[+] is an enhancer of eye phenotype of BacA\p35GMR.PH, Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+], Scer\GAL4hs.2sev is an enhancer of eye phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+], Scer\GAL4hs.2sev is an enhancer of eye phenotype of BacA\p35GMR.PH, Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+] is an enhancer of mesothoracic tergum phenotype of Nf-YAdsRNA.231-399.UAS.WIZ, Scer\GAL4pnr-MD237
bsk1 is an enhancer of embryonic epidermis | dorsal phenotype of Pkn06736
bsk1 is an enhancer of embryo | dorsal closure stage phenotype of Pkn06736
bsk1 is an enhancer of embryonic epidermis | dorsal phenotype of kay1
bsk1 is an enhancer of embryonic epidermis | dorsal phenotype of kay2
bsk1/bsk[+] is a non-enhancer of eye | somatic clone phenotype of gfr1
bsk1/bsk[+] is a non-enhancer of photoreceptor cell & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549
bsk1 is a non-enhancer of wing phenotype of Scer\GAL4dpp.PH, eyaUAS.cHa
bsk1 is a non-enhancer of phenotype of Rho1rev220
bsk1/bsk[+] is a suppressor | partially of wing phenotype of RpS3Plac92, ykiB5
bsk1/bsk[+] is a suppressor | partially of wing disc | larval stage phenotype of RpS3Plac92, ykiB5
bsk1 is a suppressor of eye photoreceptor cell | decreased number phenotype of Tace1
bsk1 is a suppressor of lipid droplet | increased number | adult stage phenotype of Tace1
bsk1/bsk[+] is a suppressor of adult midgut epithelium phenotype of Scer\GAL4NP5130, UvragHMS01357
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hepCA.UAS
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrUAS.cIa
bsk1/bsk[+] is a suppressor of rhabdomere of eye photoreceptor cell phenotype of ninaE17, ninaEP37H
bsk1 is a suppressor | partially of neuroblast | somatic clone | larval stage phenotype of fzy5032
bsk1/bsk[+] is a suppressor of wing disc phenotype of CskGD9345, Scer\GAL4ptc-559.1
bsk1 is a suppressor of wing phenotype of DaxxEY09290, Scer\GAL4Bx-MS1096
bsk1/bsk[+] is a suppressor of eye phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/hepr75 is a suppressor of eye phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+] is a suppressor of eye phenotype of Hsap\APPAβ42.GMR.Tag:SS(rPENK)
bsk1/bsk[+] is a suppressor of germline cell | embryonic stage 15 phenotype of raw155.27/raw134.47
bsk1/bsk[+] is a suppressor | partially of wing phenotype of HIV-1\VpuUAS.cLa, Scer\GAL4dpp.blk1
bsk1/bsk[+] is a suppressor | partially of eye phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS
bsk1/bsk[+] is a suppressor of eye phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk1/bsk[+] is a suppressor of ommatidium phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk1/bsk[+] is a suppressor of interommatidial bristle phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk1/bsk[+] is a suppressor of eye phenotype of hepCA.sev.Tag:MYC
bsk1/bsk[+] is a suppressor of eye phenotype of Rac1GMR.PN
bsk1/bsk[+] is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa
bsk1/bsk[+] is a suppressor of wing phenotype of flw1, pucA251.1F3/puc[+]
bsk1/bsk[+] is a suppressor | partially of embryonic/larval anterior midgut phenotype of pucGS16811
bskDN.UAS, bsk1, bsk[+], Scer\GAL4NP3392 is a suppressor of embryonic/larval anterior midgut phenotype of pucGS16811
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Scer\GAL4hs.2sev, Tak1UAS.cMa
bsk1/bsk[+] is a suppressor | partially of microchaeta phenotype of Rala35d
bsk1/bsk[+] is a suppressor of macrochaeta phenotype of Rala35d
bsk1/bsk[+] is a suppressor | partially of microchaeta phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca-537.4
bsk1/bsk[+] is a suppressor of macrochaeta phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca-537.4
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrUAS.cMa
bsk1 is a suppressor of eye phenotype of HScer\UAS.cMa/HUAS.cMa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
bsk1 is a suppressor of eye phenotype of RetMEN2B.GMR
bsk1 is a suppressor of eye phenotype of RetMEN2A.GMR
bsk1/bsk[+] is a suppressor of posterior crossvein phenotype of Scer\GAL469B, nmoc5-1.UAS
bsk1/bsk[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL469B, nmoc5-1.UAS
bsk1/bsk[+] is a suppressor | partially of ommatidium phenotype of peb1
bsk1/bsk[+] is a suppressor of ommatidium phenotype of Rac1GMR.PN
bsk1 is a suppressor of ommatidium phenotype of Rac1V12.hs.sev
bsk1/bsk[+] is a suppressor of scutum & macrochaeta phenotype of RalaS25N.UAS, Scer\GAL4sca-537.4
bsk1 is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, fzUAS.cAa
bsk1/bsk[+] is a non-suppressor of eye | somatic clone phenotype of gfr1
bsk1/bsk[+] is a non-suppressor of photoreceptor cell & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549
bsk1 is a non-suppressor of eye | ectopic phenotype of Scer\GAL4dpp.blk1, eyaUAS.cHa
bsk1 is a non-suppressor of wing phenotype of Scer\GAL4dpp.PH, eyaUAS.cHa
bsk1 is a non-suppressor of phenotype of Rho1rev220
Trpml1, bsk1/bsk[+] has neuromuscular junction | third instar larval stage phenotype
Trpml1, bsk1/bsk[+] has NMJ bouton | third instar larval stage phenotype
RagC-DEY11726, bsk1/bsk[+] has neuromuscular junction | third instar larval stage phenotype
RagC-DEY11726, bsk1/bsk[+] has NMJ bouton | third instar larval stage phenotype
bsk1, kay2 has follicle cell phenotype
bsk1, kay2 has oocyte | oogenesis stage S9 phenotype
bsk1/bsk[+], kayS135103 has follicle cell phenotype
bsk1/bsk[+], kayS135103 has oocyte | oogenesis stage S9 phenotype
bsk1/bsk[+], kay2 has follicle cell phenotype
bsk1/bsk[+], kay2 has oocyte | oogenesis stage S9 phenotype
bsk1, kayS135103 has follicle cell phenotype
bsk1, kayS135103 has oocyte | oogenesis stage S9 phenotype
bsk1/bsk[+], msn102 has embryonic/first instar larval cuticle | dorsal phenotype
bsk1 heterozygosity suppresses the increased mitotic index in the adult midgut epithelium induced by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression).
A bsk1 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in sicilyE mutants.
A bsk1 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in Aats-metFB mutants.
A bsk1 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in MarfB mutants.
Trpml1/+,bsk1/+ double heterozygotes show decreased number of NMJ boutons in third instar larvae compared to either Trpml1/+ or bsk1/+ single heterozygotes.
RagC-DEY11726/+,bsk1/+ double heterozygotes show decreased number of NMJ boutons in third instar larvae compared to either RagC-DEY11726/+ or bsk1/+ single heterozygotes.
A bsk1 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
The reduced wing phenotype caused by expression of DLPEY09290 under the control of Scer\GAL4Bx-MS1096 is suppressed by bsk1.
The rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS is weakly but significantly suppressed by bsk1/+.
A bsk1/+ background suppressed the rough eye phenotype found upon expression of TgA.Scer\UAS under the control of Scer\GAL4GMR.PS.
The cleft notum phenotype caused by expression of Nf-YAdsRNA.231-399.Scer\UAS.WIZ under the control of Scer\GAL4pnr-MD237 is enhanced by bsk1/+.
The wing defects seen in flw1/Y ; pucA251.1F3/+ flies are suppressed to wild type by bsk1/+.
bsk1/+ significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous pucGS16811 embryos.
Expression of bskDN.Scer\UAS under the control of Scer\GAL4Gap1-NP3392 significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous pucGS16811 embryos. This suppression is stronger if the embryos are also carrying bsk1/+.
81% of homozygous late stage 15 embryos show left-right asymmetry of tilting of the nuclei of the circular visceral muscle (CVM), as occurs in wild-type embryos at this stage. However, 14% of the mutant embryos show no difference between the tilting of the nuclei of the CVM cells on the right and left sides of the presumptive first midgut constriction and 5% show an inversion of the normal asymmetry, with nuclei in the left-ventral region being tilted more than those in the right-ventral region.
Heterozygosity for bsk1 partially suppresses the mutant eye phenotype caused by expression of Tak1Scer\UAS.cMa under the control of Scer\GAL4hs.2sev.
Shows no interaction with Mpk21.
Homozygosity for icsBG02577 suppresses the bsk1 'dorsal open' phenotype, reducing its incidence from approximately 60% to approximately 30%.
The frequency of dorsal holes in the cuticle seen in bsk1 embryos is partially suppressed by Sac1L2F/+. bsk1 shows no genetic interactions with Sac12107 or Sac1BG02228.
Heterozygosity for bsk1 can suppress the wing-vein defects, as well as wing shape and size defects seen in Scer\GAL469B, nmoc5-1.Scer\UAS adults.
The defects in photoreceptor cell projection patterns seen in larvae overexpressing msnEP549 under the control of Scer\GAL4GMR.PF are not affected by one copy of bsk1.
Mosaic egg chambers in which the follicle cells are homozygous for kay2 in a bsk1/+ background degenerate during stage 9 and show a complete failure of posteriorwards migration of the main body follicle cells (migration of the border cells appears unaffected). Mosaic egg chambers in which the follicle cells are homozygous for kayS135103 in a bsk1/+ background degenerate during stage 9 and show a complete failure of posteriorwards migration of the main body follicle cells (migration of the border cells appears unaffected).
The proportion of eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies with blistered wings (76%) is relatively unaffected by heterozygosity for bsk1 (69%), as is the proprotion with only mild wing defects 24% compared to (31%). (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).
The bsk1 dorsal open phenotype can be significantly rescued by heat induced expression of JraAsp.hs.sev.
Phenotype is enhanced by heat induced expression of JrabZIP.hs.sev.T:Ivir\HA1. Loss of a functional copy of aop suppresses the bsk1/Df(2L)flp147E dorsal closure phenotype.
A bsk1/+ background rescues the reduced adult eye size phenotype found in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS flies.
A hepr75 bsk1 background rescues the reduced adult eye size phenotype found in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS flies. Quantification of the ommatidia density suggests that ~47% of spreading apoptosis in R cells can be rescued by a hepr75 bsk1 double mutant background.
A bsk1 heterozygous background, in the presence of BacA\p35GMR.PH, generates additional rescue of the Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS eye phenotype compared to BacA\p35GMR.PH alone.
bsk1/+ partially suppresses the wing defects caused by expression of HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1.
The ability of animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4h.PU to survive in the presence of a concentration of G418 which is lethal to wild-type controls is partially suppressed if they are also heterozygous for bsk1.
The neuronal degeneration phenotype and decreased survival of animals expressing Hsap\HDQ93.ex1p.Scer\UAS under the control of Scer\GAL4elav-C155 is modestly suppressed in a bsk1/+ mutant background, but the effect is not statistically significant.
The dorsal hole defect, though not embryonic lethality, can be partly rescued by bskhs.PS.
Cuticle phenotypes reveal an allelic series: bskJ27 < bsk1 < bsk2 < bskflp147E.