UASt regulatory sequences drive expression of an inverted repeat.
Knockdown of Csk, through expression of CskGD9345 under the control of Scer\GAL4ptc-559.1, leads to basolateral invasion of epithelial cells into the posterior compartment.
Knockdown of Csk, through expression of CskGD9345 under the control of Scer\GAL4ptc-559.1, leads to a high rate of lethality before adult eclosion.
Adults expressing CskGD9345 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by dia[+]/dia5
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by SCARΔ37/SCAR[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by Arpc1[+]/Arpc1R337st
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by shg[+]/shg2
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by shg[+]/shgR6
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by shg[+]/shgR69
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by cpa107E/cpa[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by cpa69E/cpa[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by cpbbnd1/cpb[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, enhanceable by cpbM143/cpb[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by tsr[+]/tsrN96A
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by tumDH15/tum[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by bsk1/bsk[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by bsk2/bsk[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by Arpc1[+]/Arpc1Q25st
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by Arpc1Q25sd/Arpc1[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by zip[+]/zip1
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by zip2/zip[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by ena23/ena[+]
CskGD9345, Scer\GAL4ptc-559.1 has cell migration defective phenotype, suppressible by ena[+]/ena210
CskGD9345, GluRIIASP16, Scer\GAL4C57 has neurophysiology defective | third instar larval stage phenotype
CskGD9345, Scer\GAL4ptc-559.1, chic[+]/chic221 has cell migration defective phenotype
CskGD9345, Mmp1k04809/Mmp1[+], Scer\GAL4ptc-559.1 has cell migration defective phenotype
CskGD9345, Mmp2[+]/Mmp2EY08942, Scer\GAL4ptc-559.1 has cell migration defective phenotype
Arpc1R337st, CskGD9345 has lethal phenotype
Arpc1Q25st, CskGD9345 has lethal phenotype
CskGD9345, Scer\GAL4ptc-559.1, dock[+]/dock04723 has cell migration defective phenotype
Arpc1Q25sd, CskGD9345 has lethal phenotype
CskGD9345, captf00786 has lethal phenotype
CskGD9345, Mmp1k04809/Mmp2EY08942 has lethal phenotype
CskGD9345, Scer\GAL4ptc-559.1, captE593/capt[+] has cell migration defective phenotype
CskGD9345, Scer\GAL4ptc-559.1, captE636/capt[+] has cell migration defective phenotype
CskGD9345, Scer\GAL4ptc-559.1, captf00786/capt[+] has cell migration defective phenotype
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by dia[+]/dia5
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by SCARΔ37/SCAR[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by Arpc1[+]/Arpc1R337st
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by shg[+]/shg2
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by shg[+]/shgR6
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by shg[+]/shgR69
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by cpa107E/cpa[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by cpa69E/cpa[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by cpbbnd1/cpb[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, enhanceable by cpbM143/cpb[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by tsr[+]/tsrN96A
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by tumDH15/tum[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by bsk1/bsk[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by bsk2/bsk[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by Arpc1[+]/Arpc1Q25st
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by Arpc1Q25sd/Arpc1[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by zip[+]/zip1
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by zip2/zip[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by ena23/ena[+]
CskGD9345, Scer\GAL4ptc-559.1 has wing disc phenotype, suppressible by ena[+]/ena210
CskGD9345, Scer\GAL4ptc-559.1, chic[+]/chic221 has wing disc phenotype
CskGD9345, Mmp1k04809/Mmp1[+], Scer\GAL4ptc-559.1 has wing disc phenotype
CskGD9345, Mmp2[+]/Mmp2EY08942, Scer\GAL4ptc-559.1 has wing disc phenotype
CskGD9345, Scer\GAL4ptc-559.1, dock[+]/dock04723 has wing disc phenotype
CskGD9345, Scer\GAL4ptc-559.1, captE593/capt[+] has wing disc phenotype
CskGD9345, Scer\GAL4ptc-559.1, captE636/capt[+] has wing disc phenotype
CskGD9345, Scer\GAL4ptc-559.1, captf00786/capt[+] has wing disc phenotype
Third instar larvae with co-expression of GluRIICdsRNA.Scer\UAS.cBa and CskGD9345 driven simultaneously by Scer\GAL4elav-C155, Scer\GAL4sca.PC and Scer\GAL4C57 show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen with expression of GluRIICdsRNA.Scer\UAS.cBa alone) at the NMJ; larvae with co-expression show diminished evoked muscle excitation compared to larvae with expression of GluRIICdsRNA.Scer\UAS.cBa alone.
Larvae with expression of CskGD9345 driven in muscles by Scer\GAL4C57 show significant decreases in mEPSP (decreased quantal size) and attenuation of compensatory homeostatic increases in quantal content (as seen in GluRIIASP16/GluRIIASP16 mutants alone) at the NMJ; when expression is driven neuronally by Scer\GAL4elav-C155 the significant increase in quantal content is similar to that seen in GluRIIASP16/GluRIIASP16 mutants.
A tsrN96A heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A zip1 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A zip2 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
An ena23 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
An ena210 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A tumDH15 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A bsk1 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A bsk2 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
Reducing the gene dosage of tsr, zip, ena, or tum strongly suppresses CskGD9345-mediated Mmp1 expression and caspase activation.
A dia5 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A cpa107E heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A cpa69E heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A cpbbnd1 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A cpbM143 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A SCARΔ37 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
An Arpc1R337st heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
An Arpc1Q25st heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
An Arpc1Q25sd heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A chic221 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A dock04723 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A captE593 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A captE636 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A captf00786 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A Mmp1k04809 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A Mmp2EY08942 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A shg2 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A shgR6 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
A shgR69 heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.