FlyBase Allele Report: Dmel\cpa[69E]

General Information

Symbol

Dmel\cpa^69E

Species

D. melanogaster

Name

FlyBase ID

FBal0193693

Feature type

allele

Associated gene

Dmel\cpa

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Janody and Treisman, 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?180term.

(Janody and Treisman, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,058,738..21,058,738 [+]

Nucleotide change:

C21058738T

Amino acid change:

Q180term | cpa-PA

Reported amino acid change:

?180term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Janody and Treisman, 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of developmental disorder of mental health

CEA with cpa^107E

(Huang et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

cell lethal | partially | somatic clone

(Janody and Treisman, 2006)

lethal

(Janody and Treisman, 2006)

lethal (with cpa^43D)

(Janody and Treisman, 2006)

lethal - all die during first instar larval stage (with cpa^107E)

(Huang et al., 2020)

lethal - all die during first instar larval stage | recessive

(Huang et al., 2020)

Phenotype Manifest In

egg chamber | germline clone

(Gates et al., 2009)

eye (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^UAS.Tag:HA

(Janody and Treisman, 2006)

filopodium | increased number

(Gonçalves-Pimentel et al., 2011)

follicle cell | somatic clone

(Gates et al., 2009)

macrochaeta (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^UAS.Tag:HA

(Janody and Treisman, 2006)

oocyte | germline clone

(Gates et al., 2009)

oocyte nucleus | germline clone

(Gates et al., 2009)

wing (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^UAS.Tag:HA

(Janody and Treisman, 2006)

wing disc | somatic clone

(Janody and Treisman, 2006)

Detailed Description

Statement

Reference

Cultured primary neurons derived from mutant embryos show a significant increase in the number of filopodia compared to control neurons.

(Gonçalves-Pimentel et al., 2011)

Early development of egg chambers in females containing homozygous germline clones is relatively normal; mutant germ cells form 16-cell cysts, are encapsulated by follicle cells relatively normally and initially grow in size at a rate relatively similar to wild type. However, as they reach stage 6, mutant egg chambers become morphologically abnormal, taking on a spindle shape. The egg chambers cease increasing in size at this point and ultimately degenerate. Overall actin levels are relatively normal through stage 6/7 in the mutant egg chambers and ring canals appear to form normally.

73% of egg chambers in females containing homozygous germline clones do not contain a properly differentiate oocyte, instead having 16 nuclei that are all larger than that expected for the oocyte (32% of these egg chambers have 16 nuclei equal in size, while 68% have one nucleus that is smaller than the others, but not as small as a normal oocyte nucleus). The oocyte is not precisely positioned at the posterior in 61% of the egg chambers.

Homozygous follicle cell clones accumulate excess actin on their apical and lateral surface in early stage egg chambers. By stages 6-8, some mutant cells begin to lose their monolayer organisation, especially in clones near the posterior end of the egg chamber.

(Gates et al., 2009)

cpa^69E mutant cells within wing blade primordium clones accumulate actin filaments around the entire cell cortex. cpa^69E clones become extruded from this area of the wing disc. cpa^69E cells within notum primordium clones accumulate actin filaments near the apical cell membrane and are not extruded from the disc.

Adults expressing cpa^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL in a cpa^43D/cpa^69E background sometimes show wing, eye or bristle defects.

(Janody and Treisman, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible by Hsap\CAPZA2^K256E.UAS/Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible by Scer\GAL4^αTub84B.PL/Hsap\CAPZA2^R259L.UAS

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible | partially by Hsap\CAPZA2^K256E.UAS/Scer\GAL4^da.G32

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^da.G32/Hsap\CAPZA2^R259L.UAS

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible by Hsap\CAPZA2^UAS.cBa/Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible | partially by Hsap\CAPZA2^UAS.cBa/Scer\GAL4^da.G32

(Huang et al., 2020)

cpa^69E has increased mortality during development phenotype, suppressible by Hsap\CAPZA2^UAS.cBa/Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

Enhancer of

Statement

Reference

cpa^69E/cpa[+] is an enhancer of abnormal cell migration phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Other

Statement

Reference

Csk^GD9345, cpa^69E has lethal phenotype

(Rudrapatna et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

cpa^69E/cpa^107E is an enhancer of bristle of mesothoracic tergum phenotype of Hsap\CAPZA2^K256E.UAS, Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^69E/cpa[+] is an enhancer of bristle of mesothoracic tergum phenotype of Hsap\CAPZA2^K256E.UAS, Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^69E/cpa[+] is an enhancer of wing disc phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Other

Statement

Reference

Hsap\CAPZA2^R259L.UAS, Scer\GAL4^αTub84B.PL, cpa^69E has bristle of mesothoracic tergum phenotype

(Huang et al., 2020)

Hsap\CAPZA2^K256E.UAS, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has filamentous actin | P-stage phenotype

(Huang et al., 2020)

Hsap\CAPZA2^R259L.UAS, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has bristle of mesothoracic tergum phenotype

(Huang et al., 2020)

Hsap\CAPZA2^R259L.UAS, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has filamentous actin | P-stage phenotype

(Huang et al., 2020)

Hsap\CAPZA2^UAS.cBa, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has bristle of mesothoracic tergum phenotype

(Huang et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

A cpa^69E heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CAPZA2^UAS.cBa under the control of Scer\GAL4^αTub84B.PL in cpa^107E/cpa^69E background leads to mild defects in notum bristle, such as mild bending or split bristle, when compared to controls.

Expression of Hsap\CAPZA2^R259L.UAS under the control of Scer\GAL4^αTub84B.PL in cpa^107E/cpa^69E background leads to severe notum bristle defects (bending, branching, shortening, missing bristles as well as multiple defects of single bristle) in adults and disrupted F-actin organization in notum bristles at pupal stage when compared to controls.

Expression of Hsap\CAPZA2^K256E.UAS under the control of Scer\GAL4^αTub84B.PL in cpa^107E/cpa^69E background leads to disrupted F-actin organization in notum bristles at pupal stage when compared to controls.

Expression of Hsap\CAPZA2^R259L.UAS under the control of Scer\GAL4^αTub84B.PL in cpa^69E/+ background leads to defects in notum bristles when compared to controls.

(Huang et al., 2020)

Complementation and Rescue Data

Fails to complement

cpa^KG02261

(Janody and Treisman, 2006)

Partially rescued by

cpa^69E/cpa^43D is partially rescued by cpa^UAS.Tag:HA/Scer\GAL4^αTub84B.PL

(Janody and Treisman, 2006)

Comments

Ubiquitous expression of cpa^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL rescues the lethality of cpa^43D/cpa^69E mutants, although some of the rescued adults exhibit wing, eye or bristle defects.

(Janody and Treisman, 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cpa^69E

(Guo et al., 2024, Huang et al., 2020, Rudrapatna et al., 2014, Fernández et al., 2011, Gonçalves-Pimentel et al., 2011, Gates et al., 2009, Johnson et al., 2008, Janody and Treisman, 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

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