FlyBase Allele Report: Dmel\cpa[107E]

General Information

Symbol

Dmel\cpa^107E

Species

D. melanogaster

Name

FlyBase ID

FBal0193694

Feature type

allele

Associated gene

Dmel\cpa

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Janody and Treisman, 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?162term.

(Janody and Treisman, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,058,684..21,058,684 [+]

Nucleotide change:

C21058684T

Amino acid change:

Q162term | cpa-PA

Reported amino acid change:

?162term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Janody and Treisman, 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of developmental disorder of mental health

CEA with cpa^69E

(Huang et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

cell lethal | partially | somatic clone

(Janody and Treisman, 2006)

lethal

(Janody and Treisman, 2006)

lethal (with cpa^43D)

(Janody and Treisman, 2006)

lethal - all die during first instar larval stage (with cpa^69E)

(Huang et al., 2020)

lethal - all die during first instar larval stage | recessive

(Huang et al., 2020)

Phenotype Manifest In

eye (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^UAS.Tag:HA

(Janody and Treisman, 2006)

macrochaeta (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^UAS.Tag:HA

(Janody and Treisman, 2006)

wing (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^UAS.Tag:HA

(Janody and Treisman, 2006)

wing disc | somatic clone

(Fernández et al., 2011, Janody and Treisman, 2006)

Detailed Description

Statement

Reference

Homozygous clones in the wing disc are rounder than their wild-type twin spots.

(Fernández et al., 2011)

cpa^107E mutant clones induced at the first or second larval instar could not be recovered in the wing blade primordium, although mutant clones could develop in the remainder of the wing disc.

Adults expressing cpa^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL in a cpa^43D/cpa^107E background sometimes show wing, eye or bristle defects.

(Janody and Treisman, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible by Hsap\CAPZA2^K256E.UAS/Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible by Scer\GAL4^αTub84B.PL/Hsap\CAPZA2^R259L.UAS

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible | partially by Hsap\CAPZA2^K256E.UAS/Scer\GAL4^da.G32

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^da.G32/Hsap\CAPZA2^R259L.UAS

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible by Hsap\CAPZA2^UAS.cBa/Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^107E has increased mortality during development phenotype, suppressible by Hsap\CAPZA2^UAS.cBa/Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^69E/cpa^107E has increased mortality during development phenotype, suppressible | partially by Hsap\CAPZA2^UAS.cBa/Scer\GAL4^da.G32

(Huang et al., 2020)

Enhancer of

Statement

Reference

cpa^107E/cpa[+] is an enhancer of abnormal cell migration phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Other

Statement

Reference

Csk^GD9345, cpa^107E has lethal phenotype

(Rudrapatna et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

cpa^107E has wing disc | somatic clone phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Diap1^UAS.cHa

(Janody and Treisman, 2006)

Enhancer of

Statement

Reference

cpa^69E/cpa^107E is an enhancer of bristle of mesothoracic tergum phenotype of Hsap\CAPZA2^K256E.UAS, Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^107E/cpa[+] is an enhancer of bristle of mesothoracic tergum phenotype of Hsap\CAPZA2^K256E.UAS, Scer\GAL4^αTub84B.PL

(Huang et al., 2020)

cpa^107E/cpa[+] is an enhancer of wing disc phenotype of Csk^GD9345, Scer\GAL4^ptc-559.1

(Rudrapatna et al., 2014)

Other

Statement

Reference

Hsap\CAPZA2^R259L.UAS, Scer\GAL4^αTub84B.PL, cpa^107E has bristle of mesothoracic tergum phenotype

(Huang et al., 2020)

Hsap\CAPZA2^K256E.UAS, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has filamentous actin | P-stage phenotype

(Huang et al., 2020)

Hsap\CAPZA2^R259L.UAS, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has bristle of mesothoracic tergum phenotype

(Huang et al., 2020)

Hsap\CAPZA2^R259L.UAS, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has filamentous actin | P-stage phenotype

(Huang et al., 2020)

Hsap\CAPZA2^UAS.cBa, Scer\GAL4^αTub84B.PL, cpa^69E/cpa^107E has bristle of mesothoracic tergum phenotype

(Huang et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

A cpa^107E heterozygous mutant background enhances the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

Expression of th^Scer\UAS.cHa under the control of Scer\GAL4^αTub84B.PL enhances the survival of cpa^107E clones in the wing blade primordium.

(Janody and Treisman, 2006)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CAPZA2^UAS.cBa under the control of Scer\GAL4^αTub84B.PL in cpa^107E/cpa^69E background leads to mild defects in notum bristle, such as mild bending or split bristle, when compared to controls.

Expression of Hsap\CAPZA2^R259L.UAS under the control of Scer\GAL4^αTub84B.PL in cpa^107E/cpa^69E background leads to severe notum bristle defects (bending, branching, shortening, missing bristles as well as multiple defects of single bristle) in adults and disrupted F-actin organization in notum bristles at pupal stage when compared to controls.

Expression of Hsap\CAPZA2^K256E.UAS under the control of Scer\GAL4^αTub84B.PL in cpa^107E/cpa^69E background leads to disrupted F-actin organization in notum bristles at pupal stage when compared to controls.

Expression of Hsap\CAPZA2^R259L.UAS under the control of Scer\GAL4^αTub84B.PL in cpa^107E/+ background leads to defects in notum bristles when compared to controls.

(Huang et al., 2020)

Complementation and Rescue Data

Fails to complement

cpa^KG02261

(Janody and Treisman, 2006)

Partially rescued by

cpa^107E is partially rescued by cpa^UAS.Tag:HA/Scer\GAL4^αTub84B.PL

(Janody and Treisman, 2006)

cpa^43D/cpa^107E is partially rescued by cpa^UAS.Tag:HA/Scer\GAL4^αTub84B.PL

(Janody and Treisman, 2006)

Comments

Expression of cpa^{Scer\UAS.T:Ivir\HA1} in the wing disc, under the control of Scer\GAL4^αTub84B.PL, prevents the extrusion of cpa^107E clones from the wing blade primordium.

Ubiquitous expression of cpa^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^αTub84B.PL rescues the lethality of cpa^43D/cpa^107E mutants, although some of the rescued adults exhibit wing, eye or bristle defects.

(Janody and Treisman, 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cpa^1074E

(Johnson et al., 2008)

cpa^107E

(Huang et al., 2020, Rudrapatna et al., 2014, Fernández et al., 2011, Gates et al., 2009, Janody and Treisman, 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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