FlyBase Allele Report: Dmel\capt[E593]

General Information

Symbol

Dmel\capt^E593

Species

D. melanogaster

Name

FlyBase ID

FBal0117578

Feature type

allele

Associated gene

Dmel\capt

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Benlali et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: W141term.

(Benlali et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,153,016..1,153,016 [-]

Nucleotide change:

G1153016A

Amino acid change:

W141term | capt-PA; W500term | capt-PB; W141term | capt-PC

Reported amino acid change:

W141term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Benlali et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dys^RNAi.NH2.UAS

(Kucherenko et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | germline clone | recessive

(Benlali et al., 2000)

lethal (with Df(2L)Exel6004)

(Kahsai et al., 2016)

lethal | recessive

(Benlali et al., 2000)

visible | recessive | somatic clone

(Benlali et al., 2000)

Phenotype Manifest In

egg | germline clone

(Benlali et al., 2000)

eye | somatic clone

(Benlali et al., 2000)

eye disc | somatic clone

(Benlali et al., 2000)

eye disc morphogenetic furrow | somatic clone

(Benlali et al., 2000)

neuron & eye disc | posterior | somatic clone

(Benlali et al., 2000)

photoreceptor cell & eye disc | somatic clone

(Benlali et al., 2000)

wing margin bristle | somatic clone

(Benlali et al., 2000)

Detailed Description

Statement

Reference

Heterozygous capt^E593 mutant flies exhibit increased indirect flight muscle degeneration compare to controls.

(Kucherenko et al., 2011)

Homozygotes show lethality with a variable onset, from the embryonic to the second larval instar stage. Homozygous clones overlapping the morphogenetic furrow show premature photoreceptor differentiation. Many cells in posterior homozygous clones in the eye disc fail to differentiate as neurons and later appear to die, leaving scars in the adult eye. Eye discs in which all capt function has been removed (by inducing clones in a Minute background) show a severe disorganisation of the pattern of differentiation. Clones at the wing margin have wing margin bristles of abnormal shapes and sizes and the bristles are sometimes missing. Females containing homozygous germ line clones do not lay eggs. Mutant cells in the region of the morphogenetic furrow do not undergo the normal shape changes and instead retain large apical profiles. Apical constriction of cells at the lateral edges of mutant clones in the eye disc is seen.

(Benlali et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Csk^GD9345, Scer\GAL4^ptc-559.1, capt^E593/capt[+] has abnormal cell migration phenotype

(Rudrapatna et al., 2014)

Csk^GD9345, capt^E593 has lethal phenotype

(Rudrapatna et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

capt^E593/capt[+] is an enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

NOT Enhancer of

Statement

Reference

capt^E593/capt[+] is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Other

Statement

Reference

Csk^GD9345, Scer\GAL4^ptc-559.1, capt^E593/capt[+] has wing disc phenotype

(Rudrapatna et al., 2014)

Df(3R)Exel6184/+, capt^E593 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A capt^E593 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing Csk^GD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4^ptc-559.1.

(Rudrapatna et al., 2014)

capt Dys double heterozygous flies (capt^E593/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

capt^E593 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of capt^E593 enhances the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of capt^E593 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

capt^E593 Dg³²³ double heterozygous flies do not exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

capt^E636/capt^E593 is partially rescued by capt^C.UAS/Scer\GAL4^da.G32

(Benlali et al., 2000)

capt^E636/capt^E593 is partially rescued by Scer\GAL4^da.G32/capt^UAS.cBa

(Benlali et al., 2000)

Comments

Expression of capt^Scer\UAS.cBa under the control of Scer\GAL4^da.G32 rescues capt^E593/capt^E636 animals to viability, although the rescued adults sometimes show wing or eye defects. Expression of capt^C.Scer\UAS under the control of Scer\GAL4^da.G32 rescues capt^E593/capt^E636 animals to viability and restores normal photoreceptor differentiation.

(Benlali et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5942

w^*; capt^E593 P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

acu^E593

(Benlali et al., 2000)

capt^E593

(Rudrapatna et al., 2014, Kucherenko et al., 2011, Janody and Treisman, 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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