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General Information
Symbol
Dmel\captE593
Species
D. melanogaster
Name
FlyBase ID
FBal0117578
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G1153016A

Amino acid change:

W141term | capt-PA; W500term | capt-PB; W141term | capt-PC

Reported amino acid change:

W141term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W141term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous captE593 mutant flies exhibit increased indirect flight muscle degeneration compare to controls.

Homozygotes show lethality with a variable onset, from the embryonic to the second larval instar stage. Homozygous clones overlapping the morphogenetic furrow show premature photoreceptor differentiation. Many cells in posterior homozygous clones in the eye disc fail to differentiate as neurons and later appear to die, leaving scars in the adult eye. Eye discs in which all capt function has been removed (by inducing clones in a Minute background) show a severe disorganisation of the pattern of differentiation. Clones at the wing margin have wing margin bristles of abnormal shapes and sizes and the bristles are sometimes missing. Females containing homozygous germ line clones do not lay eggs. Mutant cells in the region of the morphogenetic furrow do not undergo the normal shape changes and instead retain large apical profiles. Apical constriction of cells at the lateral edges of mutant clones in the eye disc is seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

A captE593 heterozygous mutant background modifies the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.

capt Dys double heterozygous flies (captE593/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

captE593 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of captE593 enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of captE593 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

captE593 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of captScer\UAS.cBa under the control of Scer\GAL4da.G32 rescues captE593/captE636 animals to viability, although the rescued adults sometimes show wing or eye defects. Expression of captC.Scer\UAS under the control of Scer\GAL4da.G32 rescues captE593/captE636 animals to viability and restores normal photoreceptor differentiation.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)