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Reference
Citation
Kahsai, L., Millburn, G.H., Cook, K.R. (2016). Phenotypes Associated with Second Chromosome P Element Insertions in Drosophila melanogaster.  G3 (Bethesda) 6(8): 2665--2670.
FlyBase ID
FBrf0233125
Publication Type
Research paper
Abstract

In Drosophila melanogaster, P element transposition has been a productive means of insertional mutagenesis. Thousands of genes have been tagged with natural and engineered P element constructs. Nevertheless, chromosomes carrying P element insertions tend to have high levels of background mutations from P elements inserting and excising during transposition. Consequently, the phenotypes seen when P element-bearing chromosomes are homozygous are often not attributable to the P insertions themselves. In this study, 178 strains in the Bloomington Drosophila Stock Center collection with P insertions on the second chromosome were complementation tested against molecularly defined chromosomal deletions and previously characterized single-gene mutations to determine if recessive lethality or sterility is associated with the P insertions rather than background mutations. This information should prove valuable to geneticists using these strains for experimental studies of gene function.

PubMed ID
PubMed Central ID
PMC4978919 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    G3 (Bethesda)
    Title
    G3 : genes - genomes - genetics
    ISBN/ISSN
    2160-1836
    Data From Reference
    Aberrations (220)
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    Alleles (322)
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    Genes (272)
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    Insertions (178)