FlyBase Insertion Report: Dmel\P{PZ}wun[03497]

General Information

Symbol

Dmel\P{PZ}wun⁰³⁴⁹⁷

Species

D. melanogaster

Name

FlyBase ID

FBti0005239

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)03497

Affected gene(s)

Ecol\lacZ, wun

Viability / fertility

viable

Associated allele(s)

Ecol\lacZ⁰³⁴⁹⁷, wun⁰³⁴⁹⁷

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 45D4 )

Sequence Location

2R:9,409,655..9,409,655 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:9,409,655..9,409,655 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

45D4 (inferred by FlyBase from sequence location)

Cytological location (reported)

45D4-45D5 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025611

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

wun

(Kahsai et al., 2016, Misra, 2000.8.25)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰³⁴⁹⁷

Phenotypic Class Phenotype Manifest in Detailed Description

wun⁰³⁴⁹⁷

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval foregut

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

adult stage

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In embryo, expression in dorsal ridge appears as a row of stained cells; expression in Malpighian tubules, posterior spiracle and anal pad uncertain.

(Jackle and Janning, 1998.8.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)03497

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11333

cn¹ P{PZ}wun⁰³⁴⁹⁷/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Deficiency complementation analysis indicates that the lethality of the chromosome is separable from the P{PZ}wun⁰³⁴⁹⁷ insertion.

(Kahsai et al., 2016)

Location 2R:4921468-4921469 confirmed by FlyBase alignment of dbGSS accession AQ025611 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

The P{PZ}l(2)03497⁰³⁴⁹⁷ insertion maps 164bp upstream of the 5\' end of the CT4876 transcript of wun.

(Misra, 2000.8.25)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1333

(Meister and Braun, 1995.10)

P{PZ}l(2)03497⁰³⁴⁹⁷

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}wun⁰³⁴⁹⁷

P{ry^+t7.2=PZ}l(2)03497⁰³⁴⁹⁷

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000320

References (12)

Report Sections

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