Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
7.0 (northern blot)
None of the polypeptides share 100% sequence identity.
1668 (aa); 182 (kD predicted)
Contains two Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which may be required for an association with nuclear receptors.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Asx using the Feature Mapper tool.
Asx transcript is present at high levels in 0-1.5 hr embryos. Levels decline at 1.5-3 hours, then rise again. Levels are low in larvae, and increase in pupae and adults. Three transcripts are detected in adult males. Asx transcript is abundant in nurse cells, but absent from stage S10 oocytes. In the blastoderm stage embryo, expression is detected in a broad band in the anterior, and a narrower band in the posterior of the embryo. The transcript is ubiquitous during the rest of embryogenesis, though higher levels are detected in the neurectoderm and later in the central nervous system.
Asx protein is first detected in the cellular blastoderm embryo, in both the nucleus and cytoplasm. The protein may be at slightly higher levels in the anterior of the embryo. Later in embryogenesis, the protein is ubiquitous but non-uniform, with higher levels detected in the neurectoderm, and later in the central nervous system. Asx protein localizes to about 90 sites in polytene chromosomes.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Asx in GBrowse 2
Located on 2R.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Asx CG8787
Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 1 allele of xen has been isolated.
In an effort to subdivide the Pc-group genes functionally, the phenotypes of adult flies heterozygous for every pairwise combination of Pc-group mutation were examined. Asx, Pc, Pcl, Psc, Scm and Sce have similar functions in some imaginal tissues. Genetic interactions have been demonstrated between esc, Asx, E(Pc), Pcl, E(z) and sxc. Most duplications of Pc-group genes neither exhibit anterior transformations nor suppress the extra sex comb phenotype of Pc-group mutations, suggesting that not all Pc-group genes behave as predicted by the mass action model.
Mosaic and expression pattern analysis reveals that the Pc-group genes do not act only in a common complex or pathway: they must have some independent functions.
The bithorax complex genes are regulated by the Pc-group of genes, acting via 'Pc-group response elements' (PREs), that can work even when removed from the normal bithorax complex context. The Pc-group products apparently provide stable memory or imprinting of boundaries which are specified by gap and pair-rule regulators.
Mutations of genes in the Polycomb group (esc, E(z), Pc, ph-p, ph-d, Scm, Pcl, Sce, Asx, Psc, pho and Antp) cause abnormal segmental development due to the ectopic expression of abd-A and Abd-B. Embryos lacking both maternal and zygotic Asx product were generated to determine abd-A and Abd-B expression patterns.
Embryonic and adult phenotypes suggest that Asx is required zygotically for determination of segment number and polarity. A construct carrying an eve promoter fused to a Ecol\lacZ gene was used to demonstrate that 40% of the mutant Asx embryos exhibit some ectopic expression of eve. This suggests Asx is required for the normal expression of eve.
Pole cell transplantation techniques demonstrate that Asx is maternally expressed and is required for normal the bithorax complex expression during embryogenesis.
A member of the Polycomb group of genes. Asx function is required for the regulation of the bithorax complex during embryonic development. In its absence abd-A and Abd-B are ectopically expressed, leading to the conclusion that Asx is a negative regulator of the bithorax complex.
Asx/+ males have extra sex-comb teeth on meso- and metathoracic legs. Homozygote embryonic lethal. Abdominal denticles in head and thoracic segments; abdominal segments 1-7 transformed into more posterior segments. In double mutant combinations with Pcl, Psc, or Scm shows strong posterior transformation of all segments and failure of head involution. The presence of the bithorax complex+ required for expression of phenotype.