FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000115773
Interaction Network
Interactions Browser links
E(z) network
Asx network
Reported Interactions
FBrf0236724-2.PD.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(z)

bait

GST tag

E(z) 
Asx 

Asx

prey

[35]S label

Asx 
Asx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(z) 
SET domain
sufficient binding region
aa 626-740
Asx 
interaction domain
sufficient binding region
aa 1-354aa 1200-1501

coordinates relative to Asx-PA

coordinates relative to Asx-PA

Asx 
interaction domain
necessary binding region
aa 1-199aa 1200-1380

coordinates relative to Asx-PA

coordinates relative to Asx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0236724-5.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Asx 

Asx

bait

GST tag

Asx 
E(z) 

E(z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Asx 
interaction domain
sufficient binding region
aa 1200-1501

coordinates relative to Asx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

FBrf0236724-6.PLA
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Asx 

Asx

neutral component
Asx 

Asx

neutral component
Asx 

Asx

neutral component
E(z) 

E(z)

neutral component
E(z) 

E(z)

neutral component
E(z) 

E(z)

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; proteins produced from endogenous gene.

The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)