FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

western blot

ID (Ontology)

MI:0113 (Molecular Interactions)

Definition

Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane.[ PubMed:14755292 ]

Also Known As

"Immuno blot"

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Direct annotations: records annotated with this exact term (child terms are NOT included)

Data Class	Field	Direct annotations
Insertions (FBti)	EXPRESSION_STAGE_TISSUE_POSITION

Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Genes	Interaction Groups	Insertions	Constructs
western blot (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

feature detection method
 |__experimental feature detection_______
experimental participant identification  |
 |__identification by antibody___________|
                                         western blot  7868 rec.
                                          |__anti tag western blot 3667 rec.
                                          |__monoclonal antibody western blot
                                          |__polyclonal antibody western blot

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	identification by antibody experimental feature detection
Part of
Synonyms & Secondary IDs
Synonyms
	"Immuno blot" EXACT PSI-MI-alternate
Secondary IDs

External Crossreferences & Linkouts