FB2026_03 , released September 17, 2026
FB2026_03 , released September 17, 2026
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Term molecular sieving ID (Ontology) MI:0071 (Molecular Interactions)
Definition In sizing columns (gel filtration), the elution position of a protein or of a complex depends on its Stokes radius. Molecules with a radius that is smaller than the bead size are retained and retarded by the interaction with the matrix. The observation that two proteins, loaded on a sieving column, elute in a fraction(s) corresponding to a MW that is larger than the MW of either protein may be taken as an indication that the two proteins interact. Furthermore this technique provides a conceptually simple method for evaluating the affinity of the interaction.[ PubMed:7708014 ]
Also Known As "Gel Filtration" ; "Size Exclusion Chromatography" ; "Sizing column"
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 molecular sieving (all annotations which use CV term, excluding "NOT" statements)
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experimental interaction detection
 |__biophysical________________
biochemical                    |
 |__chromatography technology__|
                               molecular sieving  528 rec.
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Is a biophysical
chromatography technology
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  • "Gel Filtration" EXACT PSI-MI-alternate
    "Size Exclusion Chromatography" EXACT PSI-MI-alternate
    "Sizing column" EXACT PSI-MI-alternate
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