FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CycG, Asx
FlyBase ID
FBig0000105571
Interaction Network
Interactions Browser links
CycG network
Asx network
Reported Interactions
FBrf0228498-1.Y2H
Reference
Dupont et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Asx 

ASX

bait

fused to LexA DNA-binding domain

Asx 
CycG 

CyclinG

prey

fused to GAL4 activation domain

CycG 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Asx 
C-terminal region
sufficient binding region
aa 1139-1668

coordinates relative to Asx-PA

CycG 
N-terminal region
sufficient binding region
aa 1-275

coordinates relative to CycG-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Dupont et al., 2015)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Dupont et al., 2015)
FBrf0228498-2.coIP.WB
Reference
Dupont et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Asx 

ASX

bait

Myc tag

Asx 
CycG 

CycG

prey

FLAG tag

CycG 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Asx 
C-terminal region
sufficient binding region
aa 1139-1668

coordinates relative to Asx-PA

CycG 
C-terminal region
sufficient binding region
aa 131-566

coordinates relative to CycG-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Asx interacts independently with both the N- and C-terminal regions of CycG.

(Dupont et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dupont et al., 2015)
FBrf0228498-3.coIP.WB
Reference
Dupont et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
CycG 

CycG

bait

FLAG tag

CycG 
Asx 

ASX

prey

Myc tag

Asx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Asx 
C-terminal region
sufficient binding region
aa 1139-1668

coordinates relative to Asx-PA

CycG 
N-terminal region
sufficient binding region
aa 1-130

coordinates relative to CycG-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dupont et al., 2015)

Asx interacts independently with both the N- and C-terminal regions of CycG.

(Dupont et al., 2015)
FBrf0228498-4.coIP.WB
Reference
Dupont et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycG 

CycG

bait

Myc tag

Asx 

ASX

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Dupont et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)