FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
CV Term Report
Help Close All Open All
General Information
Term proximity ligation assay ID (Ontology) MI:0813 (Molecular Interactions)
Definition Upon binding of two proximity probes (usually antibodies conjugated to DNA) to the same target protein complex, the oligonucleotides on the proximity probes are brought close together. These antibody-conjugated oligonucleotides hybridize to two connector oligonucleotides that are ligated to form a circular DNA molecule. This newly formed DNA-molecule can be amplified by rolling circle amplification. The resulting single-stranded DNA-molecule collapses into a bundle, which is detectable through hybridization of fluorescently labeled complementary oligonucleotides.[ PubMed:15155907 ]
Also Known As "p elisa" ; "pELISA" ; "PLA"
Comment
Links to External Ontologies
Annotations
Records annotated with this term OR any of its CHILD TERMS
    Results list data from multiple species. Click on a button above and use the 'Filter by species' options on the resulting HitList to retrieve species-specific data.
Records annotated with this exact term (annotations to child terms are NOT included)
No relevant records available
show Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
 Full annotation statements 
Relevant FlyBase reports
 Interaction Groups
 proximity ligation assay (all annotations which use CV term, excluding "NOT" statements)     143
Spanning Tree (Parents/Children)
Only view relationship: 
biochemical
 |__affinity technology__________________
experimental participant identification  |
 |__identification by antibody___________|
                                         proximity ligation assay  143 rec.
Spanning Tree View Settings
Parents/Children
View Depth
Show hierarchy levels: for parents, for children
hide Relationships
Is a affinity technology
identification by antibody
Part of
hide Synonyms & Secondary IDs
Synonyms
  • "in situ proximity ligation assay" RELATED
    "p elisa" EXACT
    "pELISA" EXACT PSI-MI-alternate
    "PLA" EXACT PSI-MI-short
    "pLISA" RELATED
    "proximity ligation" RELATED
Secondary IDs
hide External Crossreferences & Linkouts