FB2026_03 , released September 17, 2026
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Term proximity ligation assay ID (Ontology) MI:0813 (Molecular Interactions)
Definition Upon binding of two proximity probes (usually antibodies conjugated to DNA) to the same target protein complex, the oligonucleotides on the proximity probes are brought close together. These antibody-conjugated oligonucleotides hybridize to two connector oligonucleotides that are ligated to form a circular DNA molecule. This newly formed DNA-molecule can be amplified by rolling circle amplification. The resulting single-stranded DNA-molecule collapses into a bundle, which is detectable through hybridization of fluorescently labeled complementary oligonucleotides.[ PubMed:15155907 ]
Also Known As"p elisa" ; "pELISA" ; "PLA"
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biochemical
 |__affinity technology__________________
experimental participant identification  |
 |__identification by antibody___________|
                                         proximity ligation assay  144 rec.
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  • "in situ proximity ligation assay" RELATED
    "p elisa" EXACT
    "pELISA" EXACT PSI-MI-alternate
    "PLA" EXACT PSI-MI-short
    "pLISA" RELATED
    "proximity ligation" RELATED
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