FB2026_02 , released June 18, 2026

CV Term Report

Help Close All Open All

General Information

Term

pull down

ID (Ontology)

MI:0096 (Molecular Interactions)

Definition

A specific affinity chromatography method where a molecule of interest (bait) is bound to a column, often via an affinity tag expressed as a protein fusion (GST, HIS tag and others) or chemically linked to the bait molecule . The molecule may be expressed or synthesised and purified first, often in an heterologous system, bound to the matrix at high concentration and then challenged with a solution or cellular extract containing the candidate partner molecules. Alternatively, a multi-molecular complex may be adsorbed to the resin and the retained binding molecules subsequently identified.[ PubMed:14755292 ]

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

Choose a record type to view annotations for this term and its child terms. Direct annotations and full annotation statements below also provide HitList buttons.

Results list data from multiple species. Choose a record type above to open its HitList.

Direct annotations: records annotated with this exact term (child terms are NOT included)

There are no annotations using this term.

Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
pull down (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

chromatography technology__
affinity technology________|
                           affinity chromatography technology
                            |__pull down  3062 rec.
                                |__interactome parallel affinity capture

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	affinity chromatography technology
Part of
Synonyms & Secondary IDs
Synonyms
	"affinity capture" RELATED "pulldown" RELATED
Secondary IDs

External Crossreferences & Linkouts