FlyBase Insertion Report: Dmel\P{lacW}Ctu1[k00116]

General Information

Symbol

Dmel\P{lacW}Ctu1^k00116

Species

D. melanogaster

Name

FlyBase ID

FBti0006944

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k00116

Affected gene(s)

Ctu1, Ecol\lacZ, l(2)45Ai

Viability / fertility

Associated allele(s)

Ctu1^k00116, Ecol\lacZ^{l(2)45Ai-k00116}, l(2)45Ai^k00116

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2R ( 45A3 )

Sequence Location

2R:9,097,423..9,097,423 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:9,097,423..9,097,423 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

45A3 (inferred by FlyBase from sequence location)

Cytological location (reported)

45A4-45A8 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025679

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ctu1

(Kahsai et al., 2016)

Ecol\lacZ

(BDGP Project Members, 1994-1999)

l(2)45Ai

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ctu1^k00116

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^{l(2)45Ai-k00116}

Phenotypic Class Phenotype Manifest in Detailed Description

l(2)45Ai^k00116

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k00116

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

10468

y¹ w^67c23; P{lacW}Ctu1^k00116, l(2)45Ai^k00116/CyO

Kyoto

111117

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Ctu1^k00116 /CyO y⁺

External Crossreferences and Linkouts ( 0 )

Comments

Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion. The insertion maps within the CG8078 transcription unit. However, since the insertion lies within a gene-rich region, the lethality can not yet be conclusively mapped to a single gene from these results. Thus l(2)45Ai^k00116 (representing the lethality of the chromosome) has been kept as a separate allele in FlyBase for now.

(Kahsai et al., 2016)

Location 2R:4609236-4609237 confirmed by FlyBase alignment of dbGSS accession AQ025679 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{lacW}CG8078^k00116

P{lacW}Ctu1^k00116

P{lacW}l(2)45Ai^k00116

(FlyBase, 2005, FlyBase, 1992-)

P{lacW}l(2)k00116^k00116

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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