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General Information
Symbol
Dmel\eyacli-IID
Species
D. melanogaster
Name
FlyBase ID
FBal0001705
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
eyacliIID, eyaclift1, eyaCli, eyacli2D18, eyaClift
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C6529428T

Reported nucleotide change:

C1395T

Amino acid change:

Q341term | eya-PA; Q335term | eya-PB; Q251term | eya-PC

Reported amino acid change:

Q335term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q335term.

Nucleotide substitution: C1395T.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

eyacli-IID/eya4 adults and majority of eyacli-IID/In(2LR)ETD2.2 adults are eyeless.

eya1/eya3 and eya1/eya4 adults exhibit significantly higher number of ommatidia in the eye than eya1/eyacli-IID adults.

eya2/eya4 but not eya2/eya3 adults exhibit significantly higher number of ommatidia in the eye than eya2/eyacli-IID adults.

eyacs/eyacli-IID adults exhibit greater number of ommatidia than eyacs/eyacs and eyacs/T(2;3;4)ETD4.3 adults while they exhibit fewer number of ommatidia than eyacs/eya3 and eyacs/eya4 adults.

Somatic clones of homozygous mutant eyacli-IID cells induced specifically in brain optic lobes show defects within the outer proliferation centre and form large neuroepithelial cell clusters of partial IPC-like identity.

The number of SE2 neurons is normal in eyacli-IID/Df(2L)eya10 mutant animals.

eyacli-IID/Df(2L)BSC6 embryos have abnormal lateral transverse, dorsal oblique, and ventral muscle morphology and often contain mono-nucleated muscles - indicative of a block in myoblast fusion. There are also duplications in some ventral muscle fibres. The most dramatic defect is a loss of somatic muscles. Though these defects are highly consistent, their expressivity varies among different abdominal segments.

Wild type photoreceptor clones exhibit normal targeting to eyacli-IID brain tissue in third instar larvae.

In eyacli-IID embryos, the salivary glands often stall at, or near, the turning point. When eyacli-IID salivary glands do migrate posteriorly, they are kinked and not fully elongated along the anterior-posterior axis. The somatic mesoderm of these mutants is disorganized and the morphology of the visceral mesoderm is abnormal.

Male germ cells do not proliferate in mutant embryos (in contrast to wild type, where germ cells do proliferate in XY embryos).

Tv axonal projections reach the midline, but fail to innervate the dorsal neurohemal organs in 81% of cases in eyacli-IID/Df(2L)eya10 embryos. 96% of dorsal ap-expressing (dAP) axons cross the midline in the mutant embryos (these axons do not cross the midline in wild-type embryos).

In stage 13 male eyacli-IID mutant embryos, male-specific somatic gonadal precursors are able to develop even though the somatic gonadal precursors are not maintained in these mutants. The germ cells disperse and the gonad does not coalesce.

eya1/eyacli-IID flies are eyeless or have eyes reduced to less than 1/4 normal size.

Mutant embryos have mild defects in the development of the tracheal dorsal trunk.

Bolwig's organ neurons are missing in mutant embryos.

Development of the visual system proceeds normally in mutant embryos until stage 11, when the posterior lip of the optic lobe would normally start to invaginate. Invagination of the optic lobe placode does not occur in mutant embryos.

eyacli-IID/eya1 flies may be eyeless, or may have eyes that are less than 1/4 normal size. eyacli-IID/eya3 flies are eyeless. eyacli-IID/eya3 females are fertile.

eyacli-IID/Df(2L)eya10 embryos fail to hatch. Less than 50% of eyacli-IID/eyaE3 animals survive to adulthood. Adults have eyes which are less than 1/2 normal size. eyacli-IID/eya4 flies are eyeless.

Fat body precursors do form in eyacli-IID embryos, but do not differentiate into the characteristic "ladder" structure found in wild-type stage 13 embryos.

Cells of homozygous clones in the eye disc overproliferate and fail to differentiate into neurons. The clones retain their epithelial organisation and give rise to abnormal folding of the disc. Cells in the clones subsequently die. Morphogenetic furrow initiation does not occur in clones of the eye disc that encompass the margins. Propagation of the morphogenetic furrow does not occur in homozygous clones in the eye disc. 9% of the R1, R6 and R7 cells are missing in eyes in which homozygous clones have been induced using Scer\FLP1GMR.PP.

Defect in head involution. No segmental movements. Shows abdominal transformations in combination with Pcl mutants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Phenotype Manifest In
NOT suppressed by
Statement
Reference
NOT Enhancer of
Statement
Reference

eyacli-IID is a non-enhancer of eye phenotype of pbRev3.HSPB

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The eyacli-IID Bolwig's organ phenotype is not rescued by expression of hhScer\UAS.cKa under the control of Scer\GAL4h-1J3.

The gonadal mesoderm and fat body defects seen in zfh175.26; eyacli-IID double mutant embryos are identical to that seen in zfh165.34/zfh175.26 single mutant embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by
Not rescued by
Comments

eyaGRΔIAM rescues the lethality of eyacli-IID/Df(2L)BSC354. The rescued adults have markedly smaller eyes than normal and lack ocelli. The eyes have a normal complement of rhabdomeres.

eyaGRΔPSE is generally unable to rescue the lethality of eyacli-IID/Df(2L)BSC354 animals, although rare adult escapers are seen.

One copy of eya+tGR rescues the lethality of eyacli-IID/Df(2L)BSC354 animals. The rescued adults are viable and fertile, both in males and females. The external and internal morphology of the eyes is indistinguishable from wild type. The electroretinogram of the rescued adults is normal. Photoreceptor axon projections in the brains of rescued adults appear normal.

One copy of eyaNQ.GR rescues the lethality of eyacli-IID/Df(2L)BSC354 animals. The rescued adults are viable and fertile, both in males and females. The rescued adults appear to move and fly normally. The external and internal morphology of the eyes is indistinguishable from wild type. The electroretinogram of the rescued adults is normal. Photoreceptor axon projections in the brains of rescued adults appear normal.

One copy of either eya+tGR, eyaSA.GR or eyaSDE.GR fully rescues the lethality of eyacli-IID/Df(2L)BSC354 animals. The rescued adults have eyes which are indistinguishable from wild type (both externally and in cross sections) and eye discs from late third instar larvae also appear normal. The electroretinogram of the rescued adults is normal. Photoreceptor axon projections in the brains of rescued adults and third instar larvae appear normal. Rescued females are fertile and rescued males produce wild-type numbers of progeny when mated to wild-type virgin females.

The lack of Bolwig's organ neurons seen in eyacli-IID embryos is partially rescued by eyaScer\UAS.cPa expressed under the control of Scer\GAL4h-1J3.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (18)
References (45)