Isolation and characterization of Df(2L)BSC354
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC354 was isolated as a FLP recombinase-induced recombination event involving P{XP}d11570 and PBac{WH}f07568. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d11570/PBac{WH}f07568 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC354 from the segment of P{XP}d11570 to the left of its FRT site and the segment of PBac{WH}f07568 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC354 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 26D7;26E3. It failed to complement eyacli-IID and eya2.