FB2025_02 , released April 17, 2025
Gene: Dmel\net
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General Information
Symbol
Dmel\net
Species
D. melanogaster
Name
net
Annotation Symbol
CG11450
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002931
Gene Model Status
Current
Stock Availability
46 publicly available
Gene Summary
net (net) encodes a basic helix-loop-helix protein that probably acts as a transcriptional repressor. During wing vein formation it is expressed in all interveins territories and antagonises the activity of the product of Egfr. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
21B1-21B1
Sequence location
Recombination map
2-0.5
RefSeq locus
NT_033779 REGION:82421..87387
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (10 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor activity
inferred from sequence or structural similarity with FLYBASE:HLH54F; FB:FBgn0022740
(Peyrefitte et al., 2001)
inferred from biological aspect of ancestor with PANTHER:PTN000446010
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR032660
(InterPro Project Members, 2004-)
enables E-box binding
inferred from biological aspect of ancestor with PANTHER:PTN000446010
(GO Reference Genome Project, 2011-)
enables protein dimerization activity
inferred from electronic annotation with InterPro:IPR011598, InterPro:IPR036638
(InterPro Project Members, 2004-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in imaginal disc-derived wing vein specification
inferred from mutant phenotype
(Brentrup et al., 2000)
involved_in negative regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Brentrup et al., 2000)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in anatomical structure morphogenesis
inferred from biological aspect of ancestor with PANTHER:PTN008568918
(GO Reference Genome Project, 2011-)
involved_in imaginal disc-derived wing vein morphogenesis
traceable author statement
(de Celis, 2003)
involved_in positive regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000446010
(GO Reference Genome Project, 2011-)
involved_in regulation of DNA-templated transcription
inferred from sequence or structural similarity with FLYBASE:HLH54F; FB:FBgn0022740
(Peyrefitte et al., 2001)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000446010
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
net (net) encodes a basic helix-loop-helix protein that probably acts as a transcriptional repressor. During wing vein formation it is expressed in all interveins territories and antagonises the activity of the product of Egfr. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS -
Basic helix-loop-helix (bHLH) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60 amino acid region comprising a basic DNA binding domain followed by a HLH motif formed from two amphipathic α-helices connected by a loop. bHLH transcription factors form homo- and hetero-dimeric complexes, which bind to a E box consensus sequence. (Adapted from PMID:15186484).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
net: net
thumb
net: net
Edith M. Wallace, unpublished.
Wing veins form plexus-like net; first posterior cell between L3 and L4 widens toward tip; branch missing from posterior crossvein; all veins fused at base of wing, like bi. According to Waddington [1940, J. Genet. 41: 75-139 (fig.)], spaces form between epithelial layers owing to inadequate contraction during pupal period; spaces later fuse and form extra veins (Diaz-Benjumea, Gonzalez-Gaitan, and Garcia-Bellido, 1989, Genome 31: 612-19). RK1.
Summary (Interactive Fly)

bHLH transcription factor - promotes vein production in the wing

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\net for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VPI8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.45

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
net-RB
FBtr0290323
NM_080081
2225
360
net-RC
FBtr0330637
NM_001272860
1662
360
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
net-PB
FBpp0288762
39.1
360
9.82
Q9VPI8
NP_524820
AAF51562
net-PC
FBpp0303487
39.1
360
9.82
Q9VPI8
NP_001259789
AGB92326
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

360 aa isoforms: net-PB, net-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\net using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.33

Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\net in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 32 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of net
Transgenic constructs containing regulatory region of net
Aberrations (Deficiencies and Duplications) ( 30 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die during embryonic stage, with Scer\GAL4rho.PL
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
some die during embryonic stage, with Scer\GAL4rho.PL
visible
visible, with Scer\GAL4Bx-MS1096
visible (with Df(2L)ED5878)
visible (with Df(2L)Exel6001)
visible (with Df(2L)net18)
visible (with Df(2L)net62)
visible (with Df(2L)PM4)
visible (with Df(2L)PM44)
visible (with Df(2L)PMA)
visible (with Df(2L)PMD)
visible (with netB27)
visible (with netunspecified)
visible (with netZ14)
visible | dominant
visible | recessive
visible | semidominant
wild-type
Phenotype manifest in
Allele
first posterior wing cell
first posterior wing cell (with Df(2L)net62)
marginal wing cell
marginal wing cell | distal (with Df(2L)net62)
posterior wing cell
second posterior wing cell
submarginal wing cell
submarginal wing cell | distal (with Df(2L)net62)
third posterior wing cell
third posterior wing cell (with Df(2L)net62)
third posterior wing cell (with netB27)
third posterior wing cell (with netunspecified)
third posterior wing cell (with netZ14)
wing
wing, with Scer\GAL4Bx-MS1096
wing (with Df(2L)net62)
wing vein
wing vein, with Scer\GAL4Bx-MS1096
wing vein, with Scer\GAL4nub-AC-62
wing vein (with Df(2L)ED5878)
wing vein (with Df(2L)Exel6001)
wing vein (with Df(2L)net62)
wing vein (with Df(2L)PM4)
wing vein (with Df(2L)PM44)
wing vein (with Df(2L)PMA)
wing vein (with Df(2L)PMD)
wing vein (with net2-45)
wing vein (with netCh86)
wing vein | adult stage | ectopic
wing vein | ectopic
wing vein | ectopic (with Df(2L)net18)
wing vein | ectopic (with Df(2L)net62)
wing vein | ectopic (with net2-45)
wing vein | ectopic (with netB27)
wing vein | ectopic (with netCh86)
wing vein | ectopic (with netunspecified)
wing vein | ectopic (with netZ14)
wing vein L3, with Scer\GAL4Bx-MS1096
wing vein L4, with Scer\GAL4Bx-MS1096
wing vein L5, with Scer\GAL4Bx-MS1096
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (54)
Hsap\ATOH8
8 of 14
Yes
Yes
Hsap\BHLHA15
2 of 14
No
No
1  
Hsap\BHLHE22
2 of 14
No
No
2  
Hsap\BHLHE23
2 of 14
No
No
1  
Hsap\NEUROD1
2 of 14
No
No
Hsap\NEUROD2
2 of 14
No
No
Hsap\NEUROD4
2 of 14
No
Yes
Hsap\NEUROD6
2 of 14
No
No
Hsap\NEUROG1
2 of 14
No
No
2  
Hsap\NEUROG2
2 of 14
No
No
Hsap\NEUROG3
2 of 14
No
No
1  
Hsap\OLIG1
2 of 14
No
No
Hsap\OLIG2
2 of 14
No
No
Hsap\OLIG3
2 of 14
No
No
Hsap\ASCL1
1 of 14
No
No
2  
Hsap\ASCL2
1 of 14
No
No
Hsap\ASCL3
1 of 14
No
No
Hsap\ASCL4
1 of 14
No
No
1  
Hsap\ASCL5
1 of 14
No
No
Hsap\ATOH1
1 of 14
No
No
2  
Hsap\ATOH7
1 of 14
No
No
Hsap\BHLHA9
1 of 14
No
No
Hsap\FERD3L
1 of 14
No
No
1  
Hsap\FIGLA
1 of 14
No
No
Hsap\HAND1
1 of 14
No
No
Hsap\HAND2
1 of 14
No
No
1  
Hsap\ID1
1 of 14
No
No
Hsap\LYL1
1 of 14
No
No
Hsap\MESP1
1 of 14
No
No
Hsap\MESP2
1 of 14
No
No
Hsap\MSC
1 of 14
No
No
Hsap\MSGN1
1 of 14
No
No
Hsap\MYC
1 of 14
No
No
6  
Hsap\MYCL
1 of 14
No
No
Hsap\MYCN
1 of 14
No
No
Hsap\MYF5
1 of 14
No
No
Hsap\MYF6
1 of 14
No
No
1  
Hsap\MYOD1
1 of 14
No
No
Hsap\MYOG
1 of 14
No
No
1  
Hsap\NHLH1
1 of 14
No
No
Hsap\NHLH2
1 of 14
No
No
1  
Hsap\PTF1A
1 of 14
No
No
Hsap\SCX
1 of 14
No
No
Hsap\TAL1
1 of 14
No
No
1  
Hsap\TAL2
1 of 14
No
No
1  
Hsap\TCF3
1 of 14
No
No
Hsap\TCF12
1 of 14
No
No
1  
Hsap\TCF15
1 of 14
No
No
Hsap\TCF21
1 of 14
No
No
Hsap\TCF23
1 of 14
No
No
Hsap\TCF24
1 of 14
No
No
Hsap\TFAP4
1 of 14
No
No
Hsap\TWIST1
1 of 14
No
No
1  
Hsap\TWIST2
1 of 14
No
No
1  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (47)
Rnor\Atoh8
9 of 14
Yes
Yes
Rnor\Bhlha15
2 of 14
No
No
Rnor\Neurod1
2 of 14
No
No
Rnor\Neurod4
2 of 14
No
No
Rnor\Neurod6
2 of 14
No
No
Rnor\Neurog1
2 of 14
No
No
Rnor\Neurog2
2 of 14
No
No
Rnor\Neurog3
2 of 14
No
No
Rnor\Olig1
2 of 14
No
No
Rnor\Olig2
2 of 14
No
No
Rnor\Olig3
2 of 14
No
No
Rnor\Ascl1
1 of 14
No
No
Rnor\Ascl2
1 of 14
No
No
Rnor\Ascl4
1 of 14
No
No
Rnor\Ascl5
1 of 14
No
No
Rnor\Atoh1
1 of 14
No
No
Rnor\Atoh7
1 of 14
No
No
Rnor\Bhlha9
1 of 14
No
No
Rnor\Bhlhe22
1 of 14
No
No
Rnor\Bhlhe23
1 of 14
No
No
Rnor\Ferd3l
1 of 14
No
No
Rnor\Figla
1 of 14
No
No
Rnor\Hand1
1 of 14
No
No
Rnor\Hand2
1 of 14
No
No
Rnor\Lyl1
1 of 14
No
No
Rnor\Mesp1
1 of 14
No
No
Rnor\Msc
1 of 14
No
No
Rnor\Msgn1
1 of 14
No
No
Rnor\Mycs
1 of 14
No
No
Rnor\Myf5
1 of 14
No
No
Rnor\Myf6
1 of 14
No
No
Rnor\Myod1
1 of 14
No
No
Rnor\Myog
1 of 14
No
No
Rnor\Neurod2
1 of 14
No
Yes
Rnor\Nhlh1
1 of 14
No
No
Rnor\Ptf1a
1 of 14
No
No
Rnor\Scx
1 of 14
No
No
Rnor\Tal1
1 of 14
No
No
Rnor\Tal2
1 of 14
No
No
Rnor\Tcf12
1 of 14
No
No
Rnor\Tcf15
1 of 14
No
No
Rnor\Tcf21
1 of 14
No
No
Rnor\Tcf23
1 of 14
No
No
Rnor\Tcf24
1 of 14
No
No
Rnor\Tfap4
1 of 14
No
No
Rnor\Twist1
1 of 14
No
No
Rnor\Twist2
1 of 14
No
No
Mus musculus (laboratory mouse) (49)
Mmus\Atoh8
8 of 14
Yes
Yes
Mmus\Atoh1
2 of 14
No
No
6  
Mmus\Bhlha15
2 of 14
No
No
Mmus\Neurod1
2 of 14
No
No
Mmus\Neurod2
2 of 14
No
No
Mmus\Neurod4
2 of 14
No
No
1  
Mmus\Neurod6
2 of 14
No
No
Mmus\Neurog1
2 of 14
No
No
3  
Mmus\Neurog2
2 of 14
No
No
1  
Mmus\Neurog3
2 of 14
No
No
Mmus\Olig1
2 of 14
No
No
Mmus\Olig2
2 of 14
No
No
Mmus\Olig3
2 of 14
No
No
Mmus\Ascl1
1 of 14
No
No
Mmus\Ascl3
1 of 14
No
No
Mmus\Ascl4
1 of 14
No
No
Mmus\Ascl5
1 of 14
No
No
Mmus\Atoh7
1 of 14
No
No
4  
Mmus\Bhlha9
1 of 14
No
No
Mmus\Bhlhe22
1 of 14
No
No
Mmus\Bhlhe23
1 of 14
No
No
Mmus\Ferd3l
1 of 14
No
No
Mmus\Figla
1 of 14
No
No
Mmus\Hand1
1 of 14
No
No
Mmus\Hand2
1 of 14
No
No
Mmus\Lyl1
1 of 14
No
No
Mmus\Mesp1
1 of 14
No
No
Mmus\Mesp2
1 of 14
No
No
Mmus\Msc
1 of 14
No
No
Mmus\Msgn1
1 of 14
No
No
Mmus\Myf5
1 of 14
No
No
Mmus\Myf6
1 of 14
No
No
Mmus\Myod1
1 of 14
No
No
Mmus\Myog
1 of 14
No
No
Mmus\Nhlh1
1 of 14
No
No
Mmus\Ptf1a
1 of 14
No
No
Mmus\Scx
1 of 14
No
No
Mmus\Srebf2
1 of 14
No
No
Mmus\Tal1
1 of 14
No
No
Mmus\Tal2
1 of 14
No
No
Mmus\Tcf3
1 of 14
No
No
Mmus\Tcf12
1 of 14
No
No
Mmus\Tcf15
1 of 14
No
No
Mmus\Tcf21
1 of 14
No
No
Mmus\Tcf23
1 of 14
No
No
Mmus\Tcf24
1 of 14
No
No
Mmus\Tfap4
1 of 14
No
No
Mmus\Twist1
1 of 14
No
No
Mmus\Twist2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (32)
Xtro\atoh8
7 of 13
Yes
Yes
Xtro\ascl1
1 of 13
No
No
Xtro\ascl2
1 of 13
No
No
Xtro\atoh1
1 of 13
No
No
Xtro\atoh7
1 of 13
No
No
Xtro\bhlha15
1 of 13
No
No
Xtro\bhlhe22
1 of 13
No
No
Xtro\figla
1 of 13
No
No
Xtro\hand2
1 of 13
No
No
Xtro\LOC100489188
1 of 13
No
No
Xtro\LOC100493338
1 of 13
No
No
Xtro\LOC101732842
1 of 13
No
No
Xtro\mespa
1 of 13
No
No
Xtro\mespb
1 of 13
No
No
Xtro\msgn1
1 of 13
No
No
Xtro\myf6
1 of 13
No
No
Xtro\neurod1
1 of 13
No
Yes
Xtro\neurod4
1 of 13
No
Yes
Xtro\neurod6
1 of 13
No
Yes
Xtro\neurog1
1 of 13
No
No
Xtro\neurog2
1 of 13
No
No
Xtro\neurog3
1 of 13
No
No
Xtro\olig3
1 of 13
No
No
Xtro\olig4
1 of 13
No
No
Xtro\pnpo
1 of 13
No
No
Xtro\ptf1a
1 of 13
No
No
Xtro\tal1
1 of 13
No
No
Xtro\tcf21
1 of 13
No
No
Xtro\tcf24
1 of 13
No
No
Xtro\tfap4
1 of 13
No
No
Xtro\twist1
1 of 13
No
No
Xtro\ube2e2
1 of 13
No
No
Danio rerio (Zebrafish) (52)
Drer\atoh8
9 of 14
Yes
Yes
Drer\bhlha15
2 of 14
No
No
Drer\bhlhe23
2 of 14
No
No
Drer\neurod1
2 of 14
No
No
Drer\neurod2
2 of 14
No
No
Drer\neurod4
2 of 14
No
No
Drer\neurod6a
2 of 14
No
No
Drer\neurod6b
2 of 14
No
No
Drer\neurog1
2 of 14
No
No
Drer\neurog3
2 of 14
No
No
Drer\olig1
2 of 14
No
No
Drer\olig2
2 of 14
No
No
Drer\olig4
2 of 14
No
No
Drer\si:ch211-196c10.13
2 of 14
No
No
Drer\ascl1a
1 of 14
No
No
Drer\ascl1b
1 of 14
No
No
Drer\atoh1a
1 of 14
No
No
Drer\atoh1b
1 of 14
No
No
Drer\atoh1c
1 of 14
No
No
Drer\atoh7
1 of 14
No
No
Drer\bhlha9
1 of 14
No
No
Drer\bhlhe22
1 of 14
No
No
Drer\ferd3l
1 of 14
No
No
Drer\figla
1 of 14
No
No
Drer\hand2
1 of 14
No
No
Drer\LOC110440056
1 of 14
No
No
Drer\lyl1
1 of 14
No
No
Drer\mespaa
1 of 14
No
No
Drer\mespab
1 of 14
No
No
Drer\mespba
1 of 14
No
No
Drer\mespbb
1 of 14
No
No
Drer\msc
1 of 14
No
No
Drer\msgn1
1 of 14
No
No
Drer\myf5
1 of 14
No
No
Drer\myf6
1 of 14
No
No
Drer\myod1
1 of 14
No
No
Drer\nhlh2
1 of 14
No
No
Drer\olig3
1 of 14
No
No
Drer\ptf1a
1 of 14
No
No
Drer\scxa
1 of 14
No
No
Drer\scxb
1 of 14
No
No
Drer\si:ch211-246m6.4
1 of 14
No
No
Drer\tal1
1 of 14
No
No
Drer\tal2
1 of 14
No
No
Drer\tcf15
1 of 14
No
No
Drer\tcf21
1 of 14
No
No
Drer\tcf23
1 of 14
No
No
Drer\tfap4
1 of 14
No
No
Drer\twist1a
1 of 14
No
No
Drer\twist1b
1 of 14
No
No
Drer\twist2
1 of 14
No
No
Drer\twist3
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (22)
Cele\cnd-1
2 of 14
Yes
Yes
Cele\hlh-4
2 of 14
Yes
No
Cele\hlh-16
2 of 14
Yes
No
Cele\hlh-17
2 of 14
Yes
No
Cele\hlh-31
2 of 14
Yes
No
Cele\hlh-32
2 of 14
Yes
No
Cele\ngn-1
2 of 14
Yes
No
Cele\hlh-1
1 of 14
No
No
Cele\hlh-3
1 of 14
No
No
Cele\hlh-6
1 of 14
No
No
Cele\hlh-8
1 of 14
No
No
Cele\hlh-10
1 of 14
No
No
Cele\hlh-11
1 of 14
No
No
Cele\hlh-12
1 of 14
No
No
Cele\hlh-13
1 of 14
No
No
Cele\hlh-14
1 of 14
No
No
Cele\hlh-15
1 of 14
No
No
Cele\hlh-19
1 of 14
No
No
Cele\hlh-30
1 of 14
No
No
Cele\hnd-1
1 of 14
No
No
Cele\lin-32
1 of 14
No
No
Cele\Y7A9D.1
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (25)
Agam\AgaP_AGAP009983
11 of 12
Yes
Yes
Agam\AgaP_AGAP005930
2 of 12
No
No
Agam\AgaP_AGAP007241
2 of 12
No
No
Agam\AgaP_AGAP009227
2 of 12
No
No
Agam\AgaP_AGAP013335
2 of 12
No
No
Agam\AgaP_AGAP000076
1 of 12
No
No
Agam\AgaP_AGAP000876
1 of 12
No
No
Agam\AgaP_AGAP000878
1 of 12
No
No
Agam\AgaP_AGAP001448
1 of 12
No
No
Agam\AgaP_AGAP001740
1 of 12
No
No
Agam\AgaP_AGAP001741
1 of 12
No
No
Agam\AgaP_AGAP003739
1 of 12
No
No
Agam\AgaP_AGAP003756
1 of 12
No
No
Agam\AgaP_AGAP003876
1 of 12
No
No
Agam\AgaP_AGAP004299
1 of 12
No
No
Agam\AgaP_AGAP004386
1 of 12
No
No
Agam\AgaP_AGAP004907
1 of 12
No
No
Agam\AgaP_AGAP007124
1 of 12
No
No
Agam\AgaP_AGAP007696
1 of 12
No
No
Agam\AgaP_AGAP007822
1 of 12
No
No
Agam\AgaP_AGAP007824
1 of 12
No
No
Agam\AgaP_AGAP007944
1 of 12
No
No
Agam\AgaP_AGAP011985
1 of 12
No
No
Agam\AgaP_AGAP012215
1 of 12
No
Yes
Agam\AgaP_AGAP013406
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:net. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (26)
dimm
4 of 13
Oli
4 of 13
tap
4 of 13
amos
3 of 13
ato
3 of 13
cato
2 of 13
sage
2 of 13
tx
2 of 13
ac
1 of 13
ase
1 of 13
bigmax
1 of 13
crp
1 of 13
da
1 of 13
Fer1
1 of 13
Fer2
1 of 13
Fer3
1 of 13
Hand
1 of 13
HLH3B
1 of 13
HLH4C
1 of 13
HLH54F
1 of 13
l(1)sc
1 of 13
Mnt
1 of 13
nau
1 of 13
sc
1 of 13
soul
1 of 13
twi
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    netf04249
    exacerbates  childhood spinal muscular atrophy
    modeled by Smn73Ao
    (Chang et al., 2008)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ATOH8; atonal bHLH transcription factor 8
    8 of 14
    619820
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        net
        suppressible
        ast
        (Lewis, 1945)
        net
        enhanceable
        CycG
        (Nagel et al., 2016)
        net
        enhanceable
        Egfr
        (Diaz-Benjumea and Garcia-Bellido, 1990)
        net
        suppressible
        Egfr
        (Diaz-Benjumea and Garcia-Bellido, 1990)
        net
        suppressible
        emc
        (Baonza and Garcia-Bellido, 1999)
        net
        enhanceable
        emc
        (Baonza and Garcia-Bellido, 1999)
        net
        enhanceable
        HDAC1
        (Marenda et al., 2004)
        net
        enhanceable
        Mer
        (LaJeunesse et al., 2001)
        net
        enhanceable
        mor
        (Marenda et al., 2004)
        net
        enhanceable
        nmo
        (Verheyen et al., 2001)
        net
        enhanceable
        osa
        (Terriente-Félix and de Celis, 2009, Marenda et al., 2004)
        net|px
        suppressible
        osa|brm
        (Terriente-Félix and de Celis, 2009)
        net
        suppressible
        rho
        (Brentrup et al., 2000)
        net
        enhanceable
        ru
        (Nagel et al., 2016)
        net
        suppressible
        S
        (Lewis, 1945)
        net
        suppressible
        S|ast
        (Lewis, 1945)
        net
        enhanceable
        Snr1
        (Marenda et al., 2004)
        net
        enhanceable
        speck
        (Nagel et al., 2016)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Delta
        enhanceable
        net
        (Klein and Campos-Ortega, 1992)
        Egfr
        suppressible
        net
        (Diaz-Benjumea and Garcia-Bellido, 1990)
        Egfr
        enhanceable
        net
        (Diaz-Benjumea and Garcia-Bellido, 1990)
        H
        enhanceable
        net
        (Johannes and Preiss, 2002)
        Mer
        enhanceable
        net
        (LaJeunesse et al., 2001)
        Mer
        suppressible
        net
        (LaJeunesse et al., 2001)
        nmo
        enhanceable
        net
        (Verheyen et al., 2001)
        rho
        suppressible
        net
        (Brentrup et al., 2000)
        Snr1
        enhanceable
        net
        (Marenda et al., 2004)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map
        2-0.5
        Cytogenetic map
        21B1-21B1
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        21B1-21B1
        Limits computationally determined from genome sequence between P{lacW}spenk06805&P{lacW}l(2)k13604k13604 and P{lacW}U2af38k14504
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        21A1-21B3
        (Golubovsky et al., 1978)
        Experimentally Determined Recombination Data
        Location

        2-0.5

        (FlyBase, 2016)

        2-0.0

        (Comeron et al., 2012, Meyer, 1956)

        2-0

        (Golubovsky et al., 1978, Williams, 1956)

        2-

        (Kiil, 1946)

        2-0.3

        (Neel, 1942)
        Left of (cM)
        (Golubovsky et al., 1978)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (46)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (48)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              net exhibits a two tiered control in wing vein versus intervein fate by repressing rho transcription and interfering with Egfr signalling downstream of rho.

              (Brentrup et al., 2000)

              Egfr signalling and net exert mutually antagonising activities during the specification of wing vein versus intervein fate. net is required to maintain intervein development by restricting Egfr signalling to the net-free vein regions of the wing disc.

              (Brentrup et al., 2000)

              Extra vein mutations cause enhancement of rhohs.PSt ectopic vein phenotype and suppression of the rhove-1 loss of vein phenotype.

              (Sturtevant and Bier, 1995)

              Mutants display hyperplastic phenotype, showing tissue overgrowth in mitotic recombination clones.

              (Mechler, 1994)

              A screen that identified Dominant enhancers of the wing phenotype associated with Dl9P and DlFE32 identified 4 new net alleles.

              (Klein and Campos-Ortega, 1992)

              Mutations in net cause extra longitudinal or transverse veins with plexated vein phenotypes. Px, px, net, dsr and emc belong to the same synergistic group and act synergistically against kn, fu and shf.

              (Diaz-Benjumea and Garcia-Bellido, 1990)

              Px, net, dsr, px and emc loci belong to the plexus phenotypic group within the 'excess-of-vein' mutant class. Extra veins occur parallel to and between normal veins that connect to each other by crossveins, forming plexi. Plexi are distal. Proximal regions shift to the margin leaving a central area devoid of veins. Sensilla and chaetae appear shifted. Combinations of mutations produce superadditive phenotypes.

              (Diaz-Benjumea et al., 1989)
              Relationship to Other Genes
              Source for database merge of

              Source for merge of: net shout

              (FlyBase, 1996-)
              Additional comments

              Source for merge of net shout was sequence comparison ( date:010307 ).

              (FlyBase, 1996-)
              Nomenclature History
              Source for database identify of

              Source for identity of: shout CG11450

              (Moore et al., 2000)
              Nomenclature comments
              Etymology

              The expression pattern of CG11450 overlaps with that of the bHLH transcription factor twist (twi), so the gene was named "shout" after "Twist and Shout" by Lennon and McCartney (1963).

              (Moore et al., 2000)
              Synonyms and Secondary IDs (7)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 38 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Flygut - An atlas of the Drosophila adult midgut
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              References (136)