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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000119974
Interaction Network
Interactions Browser links
net network
Snr1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0174517-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
net 

NET

bait

fused to lexA DNA-binding domain

net 
Snr1 

SNR1

prey

fused to B42 activation domain

Snr1 
Snr1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
net 
N-terminal domain
sufficient binding region
aa 1-268

construct lacking HLH domain

Snr1 
C-terminal domain
sufficient binding region
aa 240-370
Snr1 
C-terminal domain
necessary binding region
aa 241-370
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast lexA-BD/B42-AD. Snr1 and full length net do not interact in this assay.

External Crossreferences and Linkouts ( 0 )
References (1)