NET
fused to lexA DNA-binding domain
SNR1
fused to B42 activation domain
construct lacking HLH domain
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast lexA-BD/B42-AD. Snr1 and full length net do not interact in this assay.